How To: Calculate the half maximum activity point of kinetic data?

To calculate the point of half of the maximum activity of each kinetic graph where your kinetic activity is sigmoidal in nature:

1. Create a Kinetic Fit transform using the Four Parameter Fit method.
2. Create a Matrix Expression transform, under the Expression tab enter:
   
   #1:c

(#1 refers to the results from the first transform - the Kinetic Fit) and :c means the calculated c coefficient of the fit for each well.

The c coefficient of the 4 parameter fit equals the midpoint of the sigmoidal fit. If the time values were stored with your kinetic measurements then this will be the time midpoint between minimum and maximum activity of the sigmoid.  (If time measurements were not stored then this will be the cycle)

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