ࡱ>     !"#$%&/()*+,-.0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcdefghijklmnopqrstuvwxyz{|}~Root Entry`5*,AssayResults ) )VersionInfo ) )Version5;  !"#$%'()*+,-./0124578:<=DFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcefgiklmnopqrstuvwxyz{|}~ Slow Kinetics >Bq@l5@No creation nameNo template name `ResultVersions ) )Details1 ) )AssayProtocol&% ) )VersionInfo ) )Version DetailsTransformManager" ) )Template NTransforms&"MantaUsage ) )Data 3          ""##$$&&''(())**,,--..//`          ""##$$&&''(())**,,--..// D133 v 757973ED398 v 757973E D77 v 757973ED113 v 720281JD398 v 720281J D77 v 720281JD113 v 7579696D398 v 7579696  D77 v 7579696100&{5A33BF19-4D19-4E7F-A579-D829A02E5FEF}IntegralY@?1.00756100&{37C4F06C-6F78-4FE0-97CE-2F5D547EF44F} Opsonic Indexw/w(0,1)&{20AB0778-D200-426F-B351-76A214CD4F76}Pos Control1>2.0&{CAD713BB-4C8C-48AF-875F-E5581A8C2698}1.210%NegativePositive 0nmRLUd^@&{929D57B6-31A4-4CEE-A431-6EC45725308D}&{7AADD935-5746-415C-B9HLD )y)CLSIDs6Contents9hDetails(>00-9181761F866D}riSingle Point Luminometricdx? <MyResultsFolder>\<ProtocolName><ProtocolName> <ResultsFile>TahomaCalcDatay)y)MeanDetails?Template@DetailsA Integral2mʣt@`v/ m@$j@'1hp@4Zq@ʡEq@vi@0y)y)Readings y)П)TimeTemp$y)y)DetailsB0y)y)AdminDataCtWellsDataEMeanDatad;B@K7A`A@-D@K7C@ltD@~jt#K@Clm@mj@l5i@bX9g@&1p@OnNt@vd@V-L@T㥛Q@+P@ rPQ@V-O@x&1Tq@mʣt@MbXn@M`@=vd@bX9$o@ltC@-GH@A`"[K@KJ@uV.H@ktH@~Clg@X9Ȣk@KC@K7F@`Mbk@Zd;p@&Cn@ClCl@x/ah@ʡErr@Er@g@PbX9i@~jtB@~j4D@G@SO[@@5^IP@T㥛ĠP@tE@~jtJ@K7yH@vi@K7AD@1!y)y)AdminData#"h~WellsDatajMeanData Opsonic Index1>i@`a+~@^Q<@H R~@)y@s@mo@y?S?S5H#?op%?ZI?}Źb?͠2? p @f?@ǃ?^@?&@`;ma@SQ??*K?An£?|H$y?lg?&Wa?>i@T3@ea@z@=| @ҽ@0f?mކ?XAs@k \@`ٷ1{@}=@|@[@?@@@@@A@A@B@B@C@C@D@D@E@E@F@F@G@G@H@H@I@I@J@J@K@K@L@L@M@M@N@N@O@O@P@@P@P@P@Q@@Q@Q@Q@R@@R@R@R@S@@S@S@S@T@@T@T@T@U@@U@U@U@V@@V@V@V@W@@W@W@W@X@@X@X@X@Y@cn?Mb?B`"?(\?L7A`?~jt?/$?Zd;? r?$C?(\?MbX?+??l@Zd; @Zd;O @jt@S%@+N@#~j@x@^I +@}?5^@= ףp@X9v@Q@"~j@@jt@lq@jt@|?5^@-@ףp= W@y&1,@jt@Dl)@x&1@Mb@d;O@zG@x&@7A`P@S㥛 @ˡE @l @Dl@1Zd@`" @Cl@ rh@K7A` @ rh@bX9 @h|?5 @n @1Zd @X9v @x&1 @Q@L7A` @!rh@V-@~jt@RQ@V-@-@}?5^I@Zd;@S@S㥛@ r@$C@@S@v/@v/@%C?S㥛?MbX9?V-?㥛 ?J +?&1@K7?!rh?MbX?"~?Cl?S?p= ף?Pn? rh?(\?ʡE?ʡE?X9v?V-?^Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 287.357 <?Y@c@@@@@@ @"@$@&@(@*@,@.@0@1@2@3@4@5@6@7@8@9@:@;@<@=@>@?@@@@@A@A@B@B@C@C@D@D@E@E@F@F@G@G@H@H@I@I@J@J@K@K@L@L@M@M@N@N@O@O@P@@P@P@P@Q@@Q@Q@Q@R@@R@R@R@S@@S@S@S@T@@T@T@T@U@@U@U@U@V@@V@V@V@(^Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 232.412 <?Y@c@@@@@@ @"@$@&@(@*@,@.@0@1@2@3@4@5@6@7@8@9@:@;@<@=@>@?@@@@@A@A@B@B@C@C@D@D@E@E@F@F@G@G@H@H@I@I@J@J@K@K@L@L@M@M@N@N@O@O@P@@P@P@P@Q@@Q@Q@Q@R@@R@R@R@S@@S@S@S@T@@T@T@T@U@@U@U@U@V@@V@V@V@W@@W@W@W@X@@X@X@X@Y@cx&1?bX9?Zd;?ףp= ?Zd;?-?sh|??Zd;?7A`?v/?zG?X9v? rh?Q@/$@x&1@ʡE@x&1 @tV @~jt @Fx @Dl@`" @"~j@(\ @5^I  @ףp= @ʡE @333333 @x&1 @ rh @Pn@K7 @Zd;O @zG @l @L7A` @ + @M @I + @Zd;O @ @x&1 @Zd;O @+@;On @Cl@9v @Dl @jt@w/ @tV@V-@sh|? @/$@sh|?@n@x&1@$C@E@HzG@jt@^I +@jt@"~@Gz@L7A`@"~@|?5^@x&@ r@|?5^@}?5^I@X9v@?rh|@|?5^?/$@X9v?MbX9@/$?V-? rh?㥛 ?MbX9?K7?S?Cl?"~j?X9v?|?5^?)\(?Zd;O?-?Q?Zd;?bX9?ʡE?K7?^Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 215.362 <?Y@c@@@@@@ @"@$@&@(@*@,@.@0@1@2@3@4@5@6@7@8@9@:@;@<@=@>@?@@@@@A@A@B@B@C@C@D@D@E@E@F@F@G@G@H@H@I@I@J@J@K@K@L@L@M@M@N@N@O@O@P@@P@P@P@Q@@Q@Q@Q@R@@R@R@R@S@@S@S@S@T@@T@T@T@U@@U@U@U@V@@V@V@V@W@@W@W@W@X@@X@X@X@Y@cbX9ȶ?X9v?Zd;?Q?x&1?`"?rh|?E?K7A`?I +?{Gz? +??5^I ?? +?Fx@ʡE@ˡE@K7A@V- @7A`@Mb@V-@S%@= ףp=@S@(\@ʡE @{Gz@x&1 @sh|?@Zd;O @V- @Pn @333333 @Cl@V- @}?5^I @Dl @h|?5 @On @ffffff@On @x&1@^I +@Fx @Cl@Cl @bX9@K7@M@K7@V-@S@Dl@+@x@y&1@bX9@x&@(\@jt@ r@"~@B`"@@V-@K@V-?n@7A`?|?5^@ r@+?Q?~jt?sh|??S㥛?w/?I +?/$?`"? +?J +?M?uV?rh|?9v?~jt?/$?^I +?p= ף?Zd;?S㥛??y&1?'1Z?/$?7A`?^Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 262.507 <?Y@c@@@@@@ @"@$@&@(@*@,@.@0@1@2@3@4@5@6@7@8@9@:@;@<@=@>@?@W@@W@W@W@X@@X@X@X@Y@cn?x&?K7?(\?V-?L7A`?sh|??y&1?#~j?MbX9??5^I ?rh|? ףp= ?sh|??ʡE@;On@ʡE@-@B`"@oʡ @X9v @K7 @V-@zG @|?5^@q= ף@Fx@(\@uV@㥛 @tV@V- @-@RQ @d;O@B`"@Clg@MbX9@S㥛 @A`"@@V@-'@/$@ʡE@Zd;O @v/@v@|?5^@/$ @|?5^@?5^I@ +@/$@+ @Fx @K@Cl @Pn @q= ףp @+N@ r@`" @`" @)\(@V-@K @ rh @On @5^I  @}?5^I@rh| @Cl@@ + @Mb@/$@Pn@/$@Zd;@^I +@bX9@X9v@ʡE@|?5^@/$@x&1@!rh@n@X9v@RQ@#~j@S㥛?(\?"~@;On?x&?|?5^?^Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 206.972 <?Y@c@@@@@@ @"@$@&@(@*@,@.@0@1@2@3@4@5@6@7@8@9@:@;@<@=@>@?@@@@@A@A@B@B@C@C@D@D@E@E@F@F@G@G@H@H@I@I@J@J@K@K@L@L@M@M@N@N@O@O@P@@P@P@P@Q@@Q@Q@Q@R@@R@R@R@S@@S@S@S@T@@T@T@T@U@@U@U@U@V@@V@V@V@W@@W@W@W@X@@X@X@X@Y@c'1Z?x&?B`"?{Gz?Zd;?`"?㥛 ?Zd;O? rh?%C?ffffff?h|?5?L7A`?(\?J +?1Zd@On@ʡE@B`"@K7@Zd;O@l@#~j @- @Q @|?5^ @ @d;O @HzG @ʡE @S @y&1@V-@Fx@Q @v/ @uV @J + @Mb @!rh @Pn @zG@x&1 @1Zd @ r @n@/$ @$C@v/@tV@NbX9@~jt @K7@J + @l@S㥛@d;O@On@m@;On@Q@#~j@?5^I @V-@jt@Q@V-@uV@|?5^@Mb?I +@"~j?V-?}?5^I?q= ףp?-? rh?|?5^?$C?^I +?/$?uV?S?^I +?w/?~jt?jt?v?K7?/$?5^I ?(\?ʡE?K7A`?p= ף?X9v?w/?Dl?7A`?]Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 36.467  <?Y@c@@@@@@ @"@$@&@(@*@,@.@0@1@2@3@4@5@6@7@8@9@:@;@<@=@>@?@@@@@A@A@B@B@C@C@D@D@E@E@F@F@G@G@H@H@I@I@J@J@K@K@L@L@M@M@N@N@O@O@P@@P@P@P@Q@@Q@Q@Q@R@@R@R@R@S@@S@S@S@T@@T@T@T@U@@U@U@U@V@@V@V@V@W@@W@W@W@X@@X@X@X@Y@cMbX?x&?Zd;?zG?-?V-?5^I ?X9v?Pn?V-?\(\?x&1?rh|?= ףp=?bX9?x&1?L7A`?;On?-?jt?&1?/$?sh|??-?(\?Dl?\(\?Zd;?Q?Fx?%C?&1?S㥛?K7?Gz?E?!rh?9v?"~j?M?-?= ףp=?`"?jt?MbX9?tV?m?uV?L7A`?㥛 ?x&1?㥛 ??5^I ?NbX9?;On?bX9?"~?#~j?x&1?-??|?5^?/$?l? +?)\(?`"?v?w/?L7A`?n?1Zd?x&?x?{Gz?/$?d;O?;On?L7A`?x&1?7A`?Zd;?y&1?ʡE?`"?'1Z?K7A?jt?x&1?S㥛?n?)\(?v/?x&1?(\?/$?MbX?MbX?A`"?]Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 35.542  <?Y@c@@@@@@ @"@$@&@(@*@,@.@0@1@2@3@4@5@6@7@8@9@:@;@<@=@>@?@@@@@A@A@B@B@C@C@D@D@E@E@F@F@G@G@H@H@I@I@J@J@K@K@L@L@M@M@N@N@O@O@P@@P@P@P@Q@@Q@Q@Q@R@@R@R@R@S@@S@S@S@T@@T@T@T@U@@U@U@U@V@@V@V@V@W@@W@W@W@X@@X@X@X@Y@cPn?h|?5?Pn?)\(?Zd;?K7?|?5^?ʡE?x&1?ʡE?\(\?K7?T㥛 ?Gz?l?Zd;?&1?Mb?EԸ?V-?ʡE?E?V-?tV?Q?Dl?p= ף?1Zd?333333?oʡ?|?5^?ʡE?5^I ?#~j?Q?1Zd??5^I ?^I +?1Zd? +?T㥛 ?Gz?#~j?d;O?%C?#~j?'1Z?Zd;?X9v?/$?L7A`?S㥛?S㥛?%C?'1Z?Zd;O? +?"~j?'1Z?"~j?Q?S㥛?M?h|?5?bX9?333333?I +?+?bX9?7A`?!rh?9v?m?ʡE??w/?x&1?tV?K7A?x&1?V-?ˡE?L7A`?ʡE?uV?bX9?'1Z?}?5^I?MbX9?"~j?jt?Q?S㥛?7A`?(\?X9v?M?sh|??jt?]Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 41.187 <?Y@c@@@@@@ @"@$@&@(@*@,@.@0@1@2@3@4@5@6@7@8@9@:@;@<@=@>@?@@@@@A@A@B@B@C@C@D@D@E@E@F@F@G@G@H@H@I@I@J@J@K@K@L@L@M@M@N@N@O@O@P@@P@P@P@Q@@Q@Q@Q@R@@R@R@R@S@@S@S@S@T@@T@T@T@U@@U@U@U@V@@V@V@V@W@@W@W@W@X@@X@X@X@Y@cx&1?9v?Zd;?Q?-?K7?J +?oʡ?#~j?!rh?q= ףp?K7?Cl?\(\?X9v?A`"?ʡE?ʡE?-?K7A?&1?m?B`"?tV?zG?rh|?\(\?-?zG?`"?%C?K7A`?^I +?#~j?Q?ˡE?S?9v?jt?M?V-? ףp= ?V-?`"?x&1?K7?Pn?d;O?Zd;?!rh?#~j?/$?MbX9?w/?jt?x&1?K7A?㥛 ?x&1?S?Q?MbX9?x&1? r?I +?ffffff?MbX?v?x&1?S?n?;On?x&?ˡE?ffffff?'1Z?V-?tV?T㥛 ?7A`?S㥛?/$?V-?L7A`? rh?Zd;O?jt?7A`?ʡE?V-?L7A`??bX9?K7A?Q?Cl?Mb?Mb?K7?]Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 39.637 <?Y@c@@@@@@ @"@$@&@(@*@,@.@0@1@2@3@4@5@6@7@8@9@:@;@<@=@>@?@@@@@A@A@B@B@C@C@D@D@E@E@F@F@G@G@H@H@I@I@J@J@K@K@L@L@M@M@N@N@O@O@P@@P@P@P@Q@@Q@Q@Q@R@@R@R@R@S@@S@S@S@T@@T@T@T@U@@U@U@U@V@@V@V@V@W@@W@W@W@X@@X@X@X@Y@cn?h|?5?x&1? ףp= ?n?n?5^I ?x&?d;O?!rh?Q?n?$C?Gz?Mb?n?X9v?v/?V-?7A`?1Zd?n?K?7A`?(\?+?zG?&1?p= ף?d;O?5^I ?;On? r?ʡE?Q?Zd;O?T㥛 ?%C?Zd;?Zd;O?V-? ףp= ?V-?}?5^I?/$?tV?Fx?n?ʡE?^I +?&1?h|?5?w/?Cl?V-?v? +?~jt?n?S㥛?RQ?w/?&1?MbX9?㥛 ?Gz? +?m?'1Z? +?Cl?X9v?X9v?K7A`? ףp= ?Zd;?A`"?`"?}?5^I?L7A`?-?x&1?x?jt?S㥛?ʡE?tV?S㥛?ʡE?V-?K7??+?V-?HzG? +?NbX9?bX9?jt?]Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 41.282 <?Y@c@@@@@@ @"@$@&@(@*@,@.@0@1@2@3@4@5@6@7@8@9@:@;@<@=@>@?@@@@@A@A@B@B@C@C@D@D@E@E@F@F@G@G@H@H@I@I@J@J@K@K@L@L@M@M@N@N@O@O@P@@P@P@P@Q@@Q@Q@Q@R@@R@R@R@S@@S@S@S@T@@T@T@T@U@@U@U@U@V@@V@V@V@W@@W@W@W@X@@X@X@X@Y@cPn?MbX9?Zd;?p= ף?-?V-?tV?n?Zd;?|?5^?\(\?x&1?/$?= ףp=?Mb?X9v?Zd;?1Zd?EԸ?|?5^?&1?v?`"? r?\(\?rh|?ffffff?L7A`?(\?~jt?9v?~jt? r?A`"?p= ף?Pn?+?'1Z?Zd;O?$C?"~?Gz?K7A?tV?Mb? r?ˡE?Zd;?x&1?|?5^?&1?}?5^I?S?x&?ʡE?M?h|?5?㥛 ?d;O?jt?(\?S㥛?ʡE?l?I +??l?+?x&1?S?&1?X9v?On?y&1? ףp= ?Zd;O?-?`"?S?K7A?|?5^?E?V-?L7A`?}?5^I?x&1?`"?}?5^I?Fx?-?Zd;?Q?MbX9?ʡE?(\?v/?MbX9? +?jt?]Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 54.277 <?Y@c@@@@@@ @"@$@&@(@*@,@.@0@1@2@3@4@5@6@7@8@9@:@;@<@=@>@?@@@@@A@A@B@B@C@C@D@D@E@E@F@F@G@G@H@H@I@I@J@J@K@K@L@L@M@M@N@N@O@O@P@@P@P@P@Q@@Q@Q@Q@R@@R@R@R@S@@S@S@S@T@@T@T@T@U@@U@U@U@V@@V@V@V@W@@W@W@W@X@@X@X@X@Y@cx&1?Mb?~jt?Gz?L7A`?B`"?^I +?Fx?V-?S㥛??`"?Fx?Gz?On?7A`?~jt?1Zd? rh?J +?~jt?n?sh|??!rh?HzG?/$?q= ףp?K7?333333?`"?X9v?K7A`?|?5^?-?(\?tV?w/?bX9?x?M?~jt? ףp= ?A`"?sh|??x&?tV?uV?V-?7A`?Cl?#~j?Mb?X9v?E?jt?x&?|?5^?+?V-?5^I ?Q?bX9?&1?/$?Dl?Gz?bX9?%C?x&1?S?n?1Zd?E?`"?Q?x&?~jt?7A`?|?5^?K7A`??5^I ?x&1?K7?A`"?|?5^?ʡE?-?5^I ?E?}?5^I?|?5^?p= ף?NbX9?K7?{Gz?/$?Dl?Cl?uV?^Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 238.872 <?Y@c@@@@@@ @"@$@&@(@*@,@.@0@1@2@3@4@5@6@7@8@9@:@;@<@=@>@?@@@@@A@A@B@B@C@C@D@D@E@E@F@F@G@G@H@H@I@I@J@J@K@K@L@L@M@M@N@N@O@O@P@@P@P@P@Q@@Q@Q@Q@R@@R@R@R@S@@S@S@S@T@@T@T@T@U@@U@U@U@V@@V@V@V@W@@W@W@W@X@@X@X@X@Y@c'1Z?Mb?d;O?zG?-?Zd;?S㥛?d;O?5^I ?NbX9?p= ף?X9v?Mb@Q@M@Zd; @tV@K7A@?5^I@+w@`"@;O@sh|?@|?5^:@(\u@MbX9@fffff@V-@ ףp= @-F@NbX94@-@V-@n @@MbX9 @- @S㥛 @%C @h|?5@oʡ @\(\@E @;On @+ @oʡ @MbX @MbX @v @A`" @Cl@V-@#~j@㥛 @bX9@ r@ˡE@x&@$C@x&@Gz@ʡE?5^I @jt@K7@q= ףp@#~j@ʡE?V-?v?uV?7A`?K?S?q= ףp?B`"? +? rh?X9v?rh|?Cl?K7?5^I ?+?M?#~j?S㥛?Mb?Fx?^I +?"~j?\(\?NbX9?7A`?HzG?X9v?E?/$?jt?^Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 213.342 <?Y@c@@@@@@ @"@$@&@(@*@,@.@0@1@2@3@4@5@6@7@8@9@:@;@<@=@>@?@@@@@A@A@B@B@C@C@D@D@E@E@F@F@G@G@H@H@I@I@J@J@K@K@L@L@M@M@N@N@O@O@P@@P@P@P@Q@@Q@Q@Q@R@@R@R@R@S@@S@S@S@T@@T@T@T@U@@U@U@U@V@@V@V@V@W@@W@W@W@X@@X@X@X@Y@cMbX?X9v?'1Z?333333?V-?V-?S?ˡE?#~j?l?333333?Cl?S㥛?HzG?$C@'1Z@&1@;On@#~j @tV@oʡ@Mb @V- @B`"@zG@ rh@p= ף@;On @ ףp= @-'@NbX94@B`"@ +@V-@R@ʡ@sh|@MbX@Zd;O @mR@Zd;O@HzG @x&1 @ˡE @?5^I @Zd; @MbX @/$@/$ @V- @$C @#~j@jt@I +@l@ r@x@'1Z@X9v@x&1@?ʡE?J +@jt@ʡE?Gz?x?x?L7A`?MbX?-?K7A`?~jt?J +?333333?`"?S?+?v?S㥛?/$?-?h|?5?rh|?$C?Zd;O?7A`?㥛 ?Fx? +?V-?Q?%C?V-?Q?9v?/$?Dl?L7A`?^Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 201.677 <?Y@c@@@@@@ @"@$@&@(@*@,@.@0@1@2@3@4@5@6@7@8@9@:@;@<@=@>@?@@@@@A@A@B@B@C@C@D@D@E@E@F@F@G@G@H@H@I@I@J@J@K@K@L@L@M@M@N@N@O@O@P@@P@P@P@Q@@Q@Q@Q@R@@R@R@R@S@@S@S@S@T@@T@T@T@U@@U@U@U@V@@V@V@V@W@@W@W@W@X@@X@X@X@Y@cPn?I +?x&1?(\?7A`?L7A`?Mb?K7?MbX?x?ffffff?oʡ@v/@(\@/$ @Zd;O@oʡ@jt@K7A @uV@ʡE@bX9 @uV @K7A @Q@ +@)\(@x&@= ףp=@n@+@Fx@tV@n@\(\ @/$ @~jt @h|?5@Cl @S㥛 @x&@333333 @ʡE @'1Z @sh|? @x&1@NbX9 @%C@m@uV@w/@K7A@K7A`@ rh@/$@sh|?@v/?Cl?T㥛 ?/$?@;On?-?Zd;O?&1?\(\?Zd;?x&?V-?v?jt?V-?V-?5^I ??~jt?|?5^?+?l?S?v?#~j?^I +?I +?/$?n?㥛 ?Mb?1Zd? rh?5^I ?(\?9v?~jt?)\(?/$?n?/$?"~?^Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 188.397 <?Y@c@@@@@@ @"@$@&@(@*@,@.@0@1@2@3@4@5@6@7@8@9@:@;@<@=@>@?@@@@@A@A@B@B@C@C@D@D@E@E@F@F@G@G@H@H@I@I@J@J@K@K@L@L@M@M@N@N@O@O@P@@P@P@P@Q@@Q@Q@Q@R@@R@R@R@S@@S@S@S@T@@T@T@T@U@@U@U@U@V@@V@V@V@W@@W@W@W@X@@X@X@X@Y@cB`"?9v?n??7A`?B`"?v?V-?M?ʡE?Q@'1Z@$C@HzG@ʡE@y&1@K@#~j@Sc@5^I @ʡE@n@@~jt @"~ @(\ @S㥛@ @x&1@(\@Zd;@h|?5@Pn@ʡE?Pn@Q@Dl@~jt@sh|?@Dl@㥛 @n @ףp= @1Zd@oʡ@+@y&1@/$@Dl@X9v@~jt@+?E?-@S@h|?5?5^I @M?M?S?MbX?Q?Pn?~jt?x&1?d;O??Pn?x&1?~jt?NbX9?-?-?uV?}?5^I?RQ?-??5^I ?5^I ? rh?#~j?S?1Zd?}?5^I?-?Mb?Zd;?`"? rh?Zd;?}?5^I?7A`?p= ף?/$?V-??Cl?X9v?v/?A`"?^Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 265.382 <?Y@c@@@@@@ @"@$@&@(@*@,@.@0@1@2@3@4@5@6@7@8@9@:@;@<@=@>@?@@@@@A@A@B@B@C@C@D@D@E@E@F@F@G@G@H@H@I@I@J@J@K@K@L@L@M@M@N@N@O@O@P@@P@P@P@Q@@Q@Q@Q@R@@R@R@R@S@@S@S@S@T@@T@T@T@U@@U@U@U@V@@V@V@V@W@@W@W@W@X@@X@X@X@Y@cV-?9v?A`"??~jt?#~j?/$?uV? +?;On?)\(?l?l@ ףp= @Mb @ˡE @y&1@Fx @Sc@ +Y@Zd;O @v @- @~jt @q= ףp @|?5^@{Gz @Pn@(\@d;O@!rh @;On@-@Zd;O@@9v @"~ @M@ʡEs@C@K7@Q@t@L7A`e@v@tV@'1@9v@Zd;O@V-@Zd;O@S@5^I @S @m @rh|@Zd;O @E @M @ʡE@@V- @S㥛@7A`@K@Q@V-@A`"@T㥛 @E@S@5^I @㥛 @$C@(\@sh|?@?5^I ?+?y&1@!rh?ʡE@K7? rh? r?X9v?K7?S?bX9?K7A`?Cl?I +?(\?x&1?㥛 ??Zd;O?On?x?^I +?^Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 324.902 <?Y@c@@@@@@ @"@$@&@(@*@,@.@0@1@2@3@4@5@6@7@8@9@:@;@<@=@>@?@@@@@A@A@B@B@C@C@D@D@E@E@F@F@G@G@H@H@I@I@J@J@K@K@L@L@M@M@N@N@O@O@P@@P@P@P@Q@@Q@Q@Q@R@@R@R@R@S@@S@S@S@T@@T@T@T@U@@U@U@U@V@@V@V@V@W@@W@W@W@X@@X@X@X@Y@cA`"?n?-?RQ?V-? rh?Cl?L7A`?NbX9?1Zd? ףp= @Zd;@X9v@ףp= W@oʡ@V-o@jt@tV@I +@ rh@jt@d;O@rh|@ +Y@Q@Dl@(\@"~j@ffffff@-F@M@V-@sh|?@K7A@(\B@1Z@㥛 0@X9@y&1,@/$@V-@ףp= @-'@#~j<@M@V-@On@Zd;_@Zd;@|?5^@'1Z@ rh@"~j@+ @o!@Mb@&1@oʡ @/$ @x&1 @Gz @jt @S@-@"~@Q@B`"@V-@|?5^ @Zd;O@+@?5^I @rh|@v@q= ףp@!rh@Dl@/$@n@w/@x&@#~j?^I +?$C@d;O@#~j? +?Zd;O@V-?%C?rh|?zG?n?5^I ?(\?Pn?On?x?|?5^?^Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 160.972$ <?Y@c@@@@@@ @"@$@&@(@*@,@.@0@1@2@3@4@5@6@7@8@9@:@;@<@=@>@?@@@@@A@A@B@B@C@C@D@D@E@E@F@F@G@G@H@H@I@I@J@J@K@K@L@L@M@M@N@N@O@O@P@@P@P@P@Q@@Q@Q@Q@R@@R@R@R@S@@S@S@S@T@@T@T@T@U@@U@U@U@V@@V@V@V@W@@W@W@W@X@@X@X@X@Y@c?5^I ?-?X9v??X9v?%C?Zd;O?T㥛 ?NbX9?E?Gz?9v?5^I ?(\?J +?X9v?Cl?X9v?&1?y&1?/$?S?ʡE?Zd;O?333333?#~j?(\?M?RQ@x&1@-?Cl?~jt@Cl?(\@^I +?'1Z?K7A`?S?~jt?Cl?p= ף?Cl?/$?K7A?w/? rh?S㥛?+?1Zd?sh|??Zd;O?n?K? rh?K7A?MbX9?bX9?S㥛?NbX9?Q?x&?-?ʡE?Zd;?RQ@7A`@E?-?Cl?jt?K7A`?~jt?X9v@{Gz?~jt?S㥛?+?NbX9?^I +?X9v?7A`?I +?S㥛?l?A`"?Mb@/$?-?MbX? rh?@Zd;O?sh|??Q?Zd;?E?ʡE?}?5^I?]Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 57.517% <?Y@c@@@@@@ @"@$@&@(@*@,@.@0@1@2@3@4@5@6@7@8@9@:@;@<@=@>@?@@@@@A@A@B@B@C@C@D@D@E@E@F@F@G@G@H@H@I@I@J@J@K@K@L@L@M@M@N@N@O@O@P@@P@P@P@Q@@Q@Q@Q@R@@R@R@R@S@@S@S@S@T@@T@T@T@U@@U@U@U@V@@V@V@V@W@@W@W@W@X@@X@X@X@Y@cK7?Fx?uV?(\?-?7A`?sh|??ˡE?sh|?? rh?\(\?V-?m?Q?x&?`"?L7A`?;On?J +? +?;On?Zd;?`"?|?5^?Q?9v??ʡE?zG?V-?/$? rh?h|?5?#~j?)\(?ʡE?+?ʡE?K7A`?ˡE?J +?ףp= ?!rh?+?On? rh?jt?tV?d;O?Mb?#~j?Mb?v/?E?K7?y&1?S㥛?|?5^?V-?J +? ףp= ?bX9?Zd;?Zd;O?/$??/$?MbX9?x? r?Zd;O?n?Fx?-?)\(?ʡE?K7?V-?㥛 ?L7A`?rh|?ˡE?"~?V-?!rh?V-?jt?|?5^?x&1?|?5^?~jt??9v?"~? ףp= ?/$?v/?Cl?~jt?]Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 68.262& <?Y@c@@@@@@ @"@$@&@(@*@,@.@0@1@2@3@4@5@6@7@8@9@:@;@<@=@>@?@@@@@A@A@B@B@C@C@D@D@E@E@F@F@G@G@H@H@I@I@J@J@K@K@L@L@M@M@N@N@O@O@P@@P@P@P@Q@@Q@Q@Q@R@@R@R@R@S@@S@S@S@T@@T@T@T@U@@U@U@U@V@@V@V@V@W@@W@W@W@X@@X@X@X@Y@cx&1?Mb?~jt?Q?V-?I +?5^I ?n?`"?rh|?q= ףp?-?E??9v?#~j?K7?ʡE?5^I ?5^I ?L7A`?Pn?^I +?K7?p= ף?9v?333333?~jt?(\?7A`?NbX9?K7A`?5^I ??5^I ?ffffff?A`"?bX9?x&1?V-?ʡE?S㥛?p= ף?V-?h|?5?Zd;O?}?5^I?V-?V-?"~j?Cl?tV?9v?Mb?Zd;?`"?n?㥛 ?|?5^?V-? rh?)\(?Mb?oʡ?/$?$C?(\?bX9?$C?Pn?/$?ʡE?d;O?Zd;O?V-?ףp= ?y&1?J +?5^I ?%C?}?5^I?X9v?d;O?K7A`?-?S?E?|?5^?/$?-?Cl?7A`?(\?ʡE?S㥛?p= ף?y&1?Zd;O?n??5^I ?]Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 64.497' <?Y@c@@@@@@ @"@$@&@(@*@,@.@0@1@2@3@4@5@6@7@8@9@:@;@<@=@>@?@@@@@A@A@B@B@C@C@D@D@E@E@F@F@G@G@H@H@I@I@J@J@K@K@L@L@M@M@N@N@O@O@P@@P@P@P@Q@@Q@Q@Q@R@@R@R@R@S@@S@S@S@T@@T@T@T@U@@U@U@U@V@@V@V@V@W@@W@W@W@X@@X@X@X@Y@cZd;?Cl?7A`?ףp= ?`"?V-?S?jt?K7?tV?Q?K7?Cl?Gz?On?K7?ʡE?L7A`? +?S?Zd;?1Zd?S? +?\(\?/$?333333?uV?Q?uV?Mb?K? +?uV?(\?uV?+?d;O?jt?d;O? rh??!rh? +?x&1?S?V-?`"?"~?Cl?~jt?9v?9v?x&1?7A`?x&?J +? rh?K7?㥛 ?ףp= ?w/?jt?X9v?$C?HzG?v/?M?n??5^I ?oʡ?ˡE?Fx?"~?= ףp=?n??5^I ?-?|?5^?"~j?+?tV?K?`"?^I +?d;O?7A`? r?ʡE?/$?V-?Q?x&1?B`"??X9v?x&1?x&1?h|?5?]Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 69.257( <?Y@c@@@@@@ @"@$@&@(@*@,@.@0@1@2@3@4@5@6@7@8@9@:@;@<@=@>@?@@@@@A@A@B@B@C@C@D@D@E@E@F@F@G@G@H@H@I@I@J@J@K@K@L@L@M@M@N@N@O@O@P@@P@P@P@Q@@Q@Q@Q@R@@R@R@R@S@@S@S@S@T@@T@T@T@U@@U@U@U@V@@V@V@V@W@@W@W@W@X@@X@X@X@Y@cn?X9v?x??7A`?h|?5?S?E?-?sh|??Q?x&1?y&1?p= ף?On?Zd;?K7?&1?V-?S?K7?/$?V-?T㥛 ?\(\?h|?5?ffffff?~jt?{Gz?V-?M?"~? r?tV?\(\?x?MbX9?Zd;O?K7?x&1?S㥛?(\?/$?rh|?d;O??5^I ?`"?~jt?"~j?l?V-?X9v?9v?y&1?tV?oʡ?+?S㥛?B`"? +?Q?Pn?V-?/$?$C?ffffff?/$?Zd;O?1Zd?/$?d;O?K7?oʡ?-??Pn?T㥛 ?~jt?$C?T㥛 ? rh?n?K7?B`"?MbX9?Fx?S㥛? +?x&1? rh?|?5^? ףp= ?ʡE?T㥛 ?Q?'1Z?l?x&1?S㥛?]Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 63.767) <?Y@c@@@@@@ @"@$@&@(@*@,@.@0@1@2@3@4@5@6@7@8@9@:@;@<@=@>@?@@@@@A@A@B@B@C@C@D@D@E@E@F@F@G@G@H@H@I@I@J@J@K@K@L@L@M@M@N@N@O@O@P@@P@P@P@Q@@Q@Q@Q@R@@R@R@R@S@@S@S@S@T@@T@T@T@U@@U@U@U@V@@V@V@V@W@@W@W@W@X@@X@X@X@Y@cx&1?On?-?333333?K7A`?K7A`?sh|??X9v?uV?Cl?q= ףp?uV?1Zd?zG?ˡE?V-?~jt?ʡE? rh?!rh?Zd;?Zd;O?^I +?MbX9?HzG?NbX9?(\?7A`?zG?L7A`?Mb?"~?/$?J +?HzG?`"?+?'1Z?'1Z?x?|?5^??S㥛?}?5^I?ʡE?J +?L7A`?V-?K7A?v?7A`?/$?+?y&1?`"?oʡ?|?5^?㥛 ?jt?I +?(\?9v?Zd;?/$?ʡE?(\?Cl?Dl?ˡE??5^I ?ʡE?x&1?Zd;O?`"?(\?Pn?V-?V-? rh?S㥛?/$?A`"?K7?"~?S?A`"?~jt?|?5^?jt?rh|?|?5^?Q?9v?T㥛 ?zG?Zd;O?Pn?/$?jt?^Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 277.2620 <?Y@c@@@@@@ @"@$@&@(@*@,@.@0@1@2@3@4@5@6@7@8@9@:@;@<@=@>@?@@@@@A@A@B@B@C@C@D@D@E@E@F@F@G@G@H@H@I@I@J@J@K@K@L@L@M@M@N@N@O@O@P@@P@P@P@Q@@Q@Q@Q@R@@R@R@R@S@@S@S@S@T@@T@T@T@U@@U@U@U@V@@V@V@V@W@@W@W@W@X@@X@X@X@Y@cB`"?l?-??V-?~jt?S?jt??5^I ?v?(\?MbX?S㥛?Q@w/@ʡE @ʡE@V-@ r@!rhm@V-@'1@|?5^@S㥛D@Q8@m@Hz@B`"@Q@"~@X9@tV@5^I @-@Hz@Zd@?5^I@Mb@'1@Mb@K7@RQ@~j@t@%C @K7@Zd;O @MbX9@Mb@K7 @Cl @K7 @- @S @$C @!rh@Pn@y&1@Mb@On@= ףp=@A`"@!rh@#~j@-@ffffff@7A`@V-@+@ˡE@"~?A`"?K?|?5^??7A`?S?rh|?$C?S?v?`"?|?5^? +?9v?n?jt?9v?ʡE? rh?uV?Q?NbX9?-?Q?NbX9?Dl?9v?uV?^Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 330.2371 <?Y@c@@@@@@ @"@$@&@(@*@,@.@0@1@2@3@4@5@6@7@8@9@:@;@<@=@>@?@@@@@A@A@B@B@C@C@D@D@E@E@F@F@G@G@H@H@I@I@J@J@K@K@L@L@M@M@N@N@O@O@P@@P@P@P@Q@@Q@Q@Q@R@@R@R@R@S@@S@S@S@T@@T@T@T@U@@U@U@U@V@@V@V@V@W@@W@W@W@X@@X@X@X@Y@cn?On?L7A`?Q?V-?S㥛?M?V-?㥛 ?v?Gz?/$?S?HzG?%C@&1@1Zd@Zd;O@~jt@"~@ʡE@/$@-@`"@Q @Mb@(\@K@p= ף@-F@v@~jt@S㥛@`"@\(@Zd@5^I @$@x&1@C@B`"@@V-2@A`Т@NbX94@-@Zd@1Z@;On@S@d;O@S㥛@ r@/$@y&1,@v@`"y@n@Cl@ʡE@HzG@L7A` @rh|@`"@~jt@ ףp= @- @jt @J + @Zd;O @㥛 @S @|?5^@l @= ףp=@S@/$@Mb@x&1@Cl@oʡ@sh|?@Mb@%C@$C?rh|@NbX9@;On@A`"?/$?㥛 ??d;O?J +?ffffff?ʡE?Fx?Fx?㥛 ?^Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 247.6672 <?Y@c@@@@@@ @"@$@&@(@*@,@.@0@1@2@3@4@5@6@7@8@9@:@;@<@=@>@?@@@@@A@A@B@B@C@C@D@D@E@E@F@F@G@G@H@H@I@I@J@J@K@K@L@L@M@M@N@N@O@O@P@@P@P@P@Q@@Q@Q@Q@R@@R@R@R@S@@S@S@S@T@@T@T@T@U@@U@U@U@V@@V@V@V@W@@W@W@W@X@@X@X@X@Y@c^I +?I +?v?= ףp=@;On@1Zd@-@bX9@ˡE@"~ @Q@On@X9v @(\ @n@ʡE@~jt@K7@J +@J +@uV@ET@A`"@K @HzG @+ @= ףp= @y&1@ @ˡE @rh|@-@S@V-@33333@'1@ +Y@NbX94@;O@9v@A`"@zG@#~j<@V-@Clg@;On@Cl@/$ @x@V-@Cl @K7 @~jt@h|?5@%C@sh|?@Zd;@n@^I +?v/?RQ?ʡE?K7?ʡE?E?Q?ʡE?Zd;?"~?Mb?oʡ?"~j?Zd;O?S?Q?tV?J +?㥛 ?S㥛?#~j?Cl?n?~jt?K??5^I ?x&1?jt?5^I ?&1? r?-??bX9?-?zG?v/? +?Mb?"~j?^Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 135.4473 <?Y@c@@@@@@ @"@$@&@(@*@,@.@0@1@2@3@4@5@6@7@8@9@:@;@<@=@>@?@@@@@A@A@B@B@C@C@D@D@E@E@F@F@G@G@H@H@I@I@J@J@K@K@L@L@M@M@N@N@O@O@P@@P@P@P@Q@@Q@Q@Q@R@@R@R@R@S@@S@S@S@T@@T@T@T@U@@U@U@U@V@@V@V@V@W@@W@W@W@X@@X@X@X@Y@cd;O?Mb?x?Q?-?V-? rh?E?^I +?Cl?Q?Mb? r?RQ@S?Zd;@ʡE@Zd;@`"@#~j@x&1@Cl@"~@B`"@@+@333333@Fx@(\?MbX?-?/$?Zd;O?Mb?@S㥛?-@S㥛@/$@?5^I @1Zd@)\(@oʡ@;On@rh|@x&1@S㥛?S㥛?^I +?ˡE?rh|?x&1?'1Z?V-?|?5^?jt?w/?Dl??5^I ?w/?Gz?y&1?Pn?ʡE?Cl?Q?x&?Zd;?-?S㥛?K7A?"~j?Zd;?~jt?Gz?d;O?~jt?~jt?S?V-?S?ʡE?`"?jt? +?Zd;O?~jt?S㥛?Fx?㥛 ?B`"?(\?v?L7A`?= ףp=?l?5^I ?Mb?uV?^Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 160.1174 <?Y@c@@@@@@ @"@$@&@(@*@,@.@0@1@2@3@4@5@6@7@8@9@:@;@<@=@>@?@@@@@A@A@B@B@C@C@D@D@E@E@F@F@G@G@H@H@I@I@J@J@K@K@L@L@M@M@N@N@O@O@P@@P@P@P@Q@@Q@Q@Q@R@@R@R@R@S@@S@S@S@T@@T@T@T@U@@U@U@U@V@@V@V@V@W@@W@W@W@X@@X@X@X@Y@cDl?x&?B`"?Q?A`"?V-?|?5^?~jt?K7?sh|??(\?h|?5?^I +?Gz?J +?n@MbX9?Dl?K7A@K7A`@E@v/@K7@V-@ףp= @S㥛@)\(?Cl?p= ף?/$?#~j?9v?d;O?MbX?HzG@%C@tV@|?5^@v/@I +@On @ @&1 @d;O @S㥛 @y&1@Cl@Mb@Mb@tV@X9v@K7A@B`"@|?5^@+?+@n@Dl?|?5^?NbX9?{Gz?K@V-?ˡE?oʡ??;On?Fx?jt?MbX?K7?K7A`?K7A`? rh?)\(?-? rh?^I +?Mb?5^I ?㥛 ?#~j?}?5^I? +?v?7A`? +?|?5^?n? rh?7A`?zG?/$?T㥛 ?{Gz?/$?m?bX9?~jt?^Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 249.1475 <?Y@c@@@@@@ @"@$@&@(@*@,@.@0@1@2@3@4@5@6@7@8@9@:@;@<@=@>@?@@@@@A@A@B@B@C@C@D@D@E@E@F@F@G@G@H@H@I@I@J@J@K@K@L@L@M@M@N@N@O@O@P@@P@P@P@Q@@Q@Q@Q@R@@R@R@R@S@@S@S@S@T@@T@T@T@U@@U@U@U@V@@V@V@V@W@@W@W@W@X@@X@X@X@Y@cx?X9v?d;O?Gz?V-?7A`?S㥛?V-?T㥛 ?Mb?)\(?X9v?|?5^?Q@MbX9@ʡE@1Zd @x& @uV @V- @Pn @/$ @K7A` @tV @ @S㥛 @@ʡE @Gz @ˡE @S㥛 @"~@^I +@Fx@(\ @d;O@㥛 0@|?5^@o!@|?5^ @V-@= ףp=@-'@-'@w/]@K7@1Z@Mb @/$ @?5^I @X9v @A`"@- @ rh @9v @+ @oʡ @;On@X9v@y&1@{Gz@K7@J +@tV@A`"@)\(@-@K7@^I +@Pn@I +@sh|?@5^I @$C@333333?rh|@㥛 ?|?5^?X9v?S㥛?bX9?uV? +?rh|?bX9?7A`?S㥛?M?K7A?/$?rh|?)\(?x?S?RQ?d;O?Zd;?E?7A`?]Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 39.032< <?Y@c@@@@@@ @"@$@&@(@*@,@.@0@1@2@3@4@5@6@7@8@9@:@;@<@=@>@?@@@@@A@A@B@B@C@C@D@D@E@E@F@F@G@G@H@H@I@I@J@J@K@K@L@L@M@M@N@N@O@O@P@@P@P@P@Q@@Q@Q@Q@R@@R@R@R@S@@S@S@S@T@@T@T@T@U@@U@U@U@V@@V@V@V@W@@W@W@W@X@@X@X@X@Y@cOn?ʡE?A`"?ףp= ?L7A`?V-?V-?Fx?d;O?jt?zG?'1Z?$C?(\?MbX9?n?K7?1Zd?|?5^?V-?K7?n?Mb?tV??T㥛 ?\(\?L7A`?zG?"~j?$C?K7A`?S㥛?"~?q= ףp?Zd;O?J +?MbX?y&1?X9v?#~j?(\?K7?"~j?'1Z?"~j?y&1?'1Z?MbX?h|?5?jt?Cl?T㥛 ?Mb?;On?ʡE?sh|??T㥛 ?x&1?`"?Q?I +?'1Z?!rh?w/?)\(?5^I ? +?v?|?5^?Cl?v/?Cl?jt?Q?x&1?E?Pn?|?5^?x&1?S?ʡE?K7A?'1Z?uV?ʡE?;On?!rh?ʡE?!rh?d;O?Q?Dl?-?)\(?Mb?J +?m?V-?]Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 48.562= <?Y@c@@@@@@ @"@$@&@(@*@,@.@0@1@2@3@4@5@6@7@8@9@:@;@<@=@>@?@@@@@A@A@B@B@C@C@D@D@E@E@F@F@G@G@H@H@I@I@J@J@K@K@L@L@M@M@N@N@O@O@P@@P@P@P@Q@@Q@Q@Q@R@@R@R@R@S@@S@S@S@T@@T@T@T@U@@U@U@U@V@@V@V@V@W@@W@W@W@X@@X@X@X@Y@cPn?9v?x&1?RQ?x&1?7A`?-?Zd;O?x&1?!rh?\(\?On?v?Q?X9v?x&1?ʡE?ʡE?jt?V-?ʡE?y&1?S㥛?K7?Q?l?Gz?K7A`?Q?#~j?/$?-? rh?"~j?\(\?Pn?+?'1Z?ˡE?x&1?tV?zG?-?#~j?w/?`"?ˡE?tV?ʡE?m?y&1?Mb?/$?X9v?`"?x?T㥛 ?S㥛?K7A?V-?(\?/$?x&1?Cl?MbX9?333333?w/?S?x&1?+?x&1?x&?&1?L7A`?RQ?y&1?#~j?#~j?S?"~?S㥛?Zd;?~jt?A`"?h|?5?n?jt?|?5^?x&?S㥛?`"?ףp= ?m?-??NbX9?Mb?l?B`"?]Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 54.712> <?Y@c@@@@@@ @"@$@&@(@*@,@.@0@1@2@3@4@5@6@7@8@9@:@;@<@=@>@?@@@@@A@A@B@B@C@C@D@D@E@E@F@F@G@G@H@H@I@I@J@J@K@K@L@L@M@M@N@N@O@O@P@@P@P@P@Q@@Q@Q@Q@R@@R@R@R@S@@S@S@S@T@@T@T@T@U@@U@U@U@V@@V@V@V@W@@W@W@W@X@@X@X@X@Y@cd;O?n?K7?Gz?`"?x&1? rh?X9v?L7A`?V-?zG?A`"?n?Gz?ʡE?On?K7?Zd;O?"~?tV?1Zd?Cl?K? r?ffffff?l?= ףp=?K?{Gz?-?9v?K7? r?tV? ףp= ?jt? +?/$?On?ˡE?+?HzG?7A`?!rh?Zd;?5^I ?`"?#~j?d;O?l?V-?bX9?Dl?x&1?L7A`?E?|?5^?I +?7A`?㥛 ?)\(?Cl?x?Cl?/$?)\(?Cl?/$?n?rh|?n?E?ʡE?V-?)\(?x?~jt?B`"?S㥛?S㥛?S㥛?&1?#~j?jt?5^I ?n?-? r?y&1?rh|?-?ףp= ?X9v?V-?Gz?bX9?l?Dl?h|?5?]Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 52.592? <?Y@c@@@@@@ @"@$@&@(@*@,@.@0@1@2@3@4@5@6@7@8@9@:@;@<@=@>@?@@@@@A@A@B@B@C@C@D@D@E@E@F@F@G@G@H@H@I@I@J@J@K@K@L@L@M@M@N@N@O@O@P@@P@P@P@Q@@Q@Q@Q@R@@R@R@R@S@@S@S@S@T@@T@T@T@U@@U@U@U@V@@V@V@V@W@@W@W@W@X@@X@X@X@Y@cbX9ȶ?Mb?x&1?Q?uV?x&1?rh|?x&?A`"?rh|??L7A`?Fx??9v?'1Z?L7A`?K7?-? rh?ʡE?Cl?K?5^I ?Q?/$?Q?ʡE? ףp= ?tV?9v?K?㥛 ?`"?Gz?Pn?/$?x&1?x?l?S㥛?(\?-?-?ˡE? rh?"~j?ʡE?K7A?MbX?K7A?h|?5?m?ʡE?~jt?/$?J +?S㥛?A`"?S㥛?Q?/$?E?bX9?Cl??X9v?bX9?x&?!rh?ˡE?x&1?Zd;?L7A`??Zd;O?~jt?jt?S㥛?K7?$C?x?B`"?K?S㥛?n?K7A`?rh|?On?|?5^?uV?Gz?MbX?sh|??Gz?w/?Cl?Cl?V-?]Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 48.362@ <?Y@c@@@@@@ @"@$@&@(@*@,@.@0@1@2@3@4@5@6@7@8@9@:@;@<@=@>@?@@@@@A@A@B@B@C@C@D@D@E@E@F@F@G@G@H@H@I@I@J@J@K@K@L@L@M@M@N@N@O@O@P@@P@P@P@Q@@Q@Q@Q@R@@R@R@R@S@@S@S@S@T@@T@T@T@U@@U@U@U@V@@V@V@V@W@@W@W@W@X@@X@X@X@Y@cx?bX9?x?HzG?x&1?K7? +?n? rh?|?5^?zG?uV?/$?\(\?9v?A`"?&1?Zd;?-?-?&1?/$?B`"?K7?(\?/$?zG?1Zd?(\?x?v/?K7?J +?~jt?zG?Zd;O?S?M?ʡE?Mb?-?Q?jt?-?l? r?uV?jt?K7?$C?V-?m?+?%C?ʡE?'1Z?!rh? rh?y&1?+? ףp= ?Mb?ʡE?$C?X9v?zG?v/?9v?Zd;?S?n?x&1?ˡE?V-?\(\?Zd;?V-?L7A`?rh|?L7A`?J +?ʡE?jt?`"?5^I ?d;O?tV?h|?5?x&?S?`"??X9v?~jt?ףp= ?NbX9?bX9?/$?#~j?]Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 49.532A <?Y@c@@@@@@ @"@$@&@(@*@,@.@0@1@2@3@4@5@6@7@8@9@:@;@<@=@>@?@@@@@A@A@B@B@C@C@D@D@E@E@F@F@G@G@H@H@I@I@J@J@K@K@L@L@M@M@N@N@O@O@P@@P@P@P@Q@@Q@Q@Q@R@@R@R@R@S@@S@S@S@T@@T@T@T@U@@U@U@U@V@@V@V@V@W@@W@W@W@X@@X@X@X@Y@cK7?X9v?A`"?(\?L7A`?uV?㥛 ?ˡE?d;O?!rh?Q?B`"?;On?p= ף?Mb?'1Z?7A`?Zd;?V-?V-?;On?/$?~jt?7A`?(\?rh|?(\?Zd;?p= ף?tV?Mb?A`"?Mb?"~?ffffff?y&1?I +?x?ˡE?x&1?"~j?333333?jt?jt?x?"~?`"?x?L7A`?/$?&1?$C?I +?m?'1Z?ˡE?h|?5? rh?B`"?jt?ffffff?S㥛?Zd;O?NbX9?v?p= ף?Cl?Dl?x?T㥛 ?On?n?Fx?#~j?= ףp=?&1?K7?-?+?uV?|?5^?oʡ?"~?-?J +?ˡE?A`"?S?Zd;?+?~jt?Q?Dl?jt?Q?v/?v?w/?A`"?^Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 189.232H <?Y@c@@@@@@ @"@$@&@(@*@,@.@0@1@2@3@4@5@6@7@8@9@:@;@<@=@>@?@@@@@A@A@B@B@C@C@D@D@E@E@F@F@G@G@H@H@I@I@J@J@K@K@L@L@M@M@N@N@O@O@P@@P@P@P@Q@@Q@Q@Q@R@@R@R@R@S@@S@S@S@T@@T@T@T@U@@U@U@U@V@@V@V@V@W@@W@W@W@X@@X@X@X@Y@cn?MbX9?V-?333333?Zd;?V-?rh|?Zd;O?uV?㥛 ?{Gz?L7A`?V-?Q?uV?bX9?$C?w/?Zd;O?y&1?;On@MbX9@7A`@~jt@@J +@{Gz@n@(\@Fx@S㥛@;On@A`"@n@HzG@l@K7A`@S@v/@T㥛 @x&1@ףp= @ʡE@ʡE@S㥛@Zd;O@MbX@Mb@MbX@tV@X9v@K7A`@tV@S@M@^I +@1Zd@Zd;O@NbX9@ˡE@ ףp= ?K7A`@T㥛 @uV@7A`@@A`"@V-@}?5^I@E@jt?|?5^@T㥛 @NbX9@ףp= ?~jt?+? rh?M?S?Cl?~jt?S?I +?X9v?-?+?/$?~jt?w/?T㥛 ?(\?Zd;?S㥛?Gz?n?Zd;O?ʡE?h|?5?^Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 221.087I <?Y@c@@@@@@ @"@$@&@(@*@,@.@0@1@2@3@4@5@6@7@8@9@:@;@<@=@>@?@@@@@A@A@B@B@C@C@D@D@E@E@F@F@G@G@H@H@I@I@J@J@K@K@L@L@M@M@N@N@O@O@P@@P@P@P@Q@@Q@Q@Q@R@@R@R@R@S@@S@S@S@T@@T@T@T@U@@U@U@U@V@@V@V@V@W@@W@W@W@X@@X@X@X@Y@cOn?X9v?K7?)\(?B`"?n?!rh?X9v?B`"?S?\(\?#~j?tV?ףp= ?A`"?Mb?$C?9v?n?uV@v?v@tV@L7A`@Q@I +@@&1 @Q @Zd; @S㥛 @n@- @x&1 @(\ @MbX9 @V- @ rh@Mb @S@n @Q@&1 @'1Z @S㥛 @x @Mb@/$ @l@V- @Cl @B`"@tV@S㥛@/$@}?5^I@ˡE@n@NbX9@x&1@(\@K7A`@5^I @#~j@K@(\@jt@tV@ +@n@|?5^@+@+@Mb@Q@|?5^@Cl@/$@Pn@$C@Pn@S㥛@}?5^I?}?5^I?Cl?~jt?S㥛?MbX9?jt?NbX9?}?5^I?q= ףp?n? +?ףp= ?x&1?Zd;?'1Z?S㥛?]Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 38.182T <?Y@c@@@@@@ @"@$@&@(@*@,@.@0@1@2@3@4@5@6@7@8@9@:@;@<@=@>@?@@@@@A@A@B@B@C@C@D@D@E@E@F@F@G@G@H@H@I@I@J@J@K@K@L@L@M@M@N@N@O@O@P@@P@P@P@Q@@Q@Q@Q@R@@R@R@R@S@@S@S@S@T@@T@T@T@U@@U@U@U@V@@V@V@V@W@@W@W@W@X@@X@X@X@Y@cx?X9v?x&1??Zd;?K7?-?n?d;O?S?Gz?V-?m?q= ףp?X9v?-?Zd;?/$?tV?-?ʡE?/$? rh?uV?\(\?/$?Q?ʡE?Q?oʡ?MbX?K7?S㥛?#~j?ffffff?On?J +?MbX?n?v?7A`?{Gz?jt?Zd;O?%C?"~j?d;O?Zd;?A`"?S㥛?V-?5^I ?S㥛?x&1?-?Mb?-??5^I ?Zd;?-?Gz?!rh?l?Cl? +?zG?M?w/?Zd;?7A`?X9v?Pn?x&1?jt?Q?MbX9?Zd;?jt?V-?jt?+?/$?ʡE?V-?sh|??x?-?#~j?Zd;O?V-?uV?Q?w/?x&1?Q?MbX?Cl?Mb?x&?]Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 44.197U <?Y@c@@@@@@ @"@$@&@(@*@,@.@0@1@2@3@4@5@6@7@8@9@:@;@<@=@>@?@@@@@A@A@B@B@C@C@D@D@E@E@F@F@G@G@H@H@I@I@J@J@K@K@L@L@M@M@N@N@O@O@P@@P@P@P@Q@@Q@Q@Q@R@@R@R@R@S@@S@S@S@T@@T@T@T@U@@U@U@U@V@@V@V@V@W@@W@W@W@X@@X@X@X@Y@cx?MbX9?`"?Gz?K7?K7? rh?ʡE?Zd;? rh?Q?Zd;?$C?q= ףp?Mb?B`"?ʡE?#~j?5^I ?S?Zd;?Cl?A`"? +?Gz?/$?(\?#~j?333333?n?$C?~jt?^I +?K7?q= ףp?Zd;O??5^I ?%C?ʡE?v/?"~? ףp= ?jt?V-?l?`"?K7A?ˡE?d;O?}?5^I?x&1? +?!rh?n?oʡ?Zd;?V-?V-?n?7A`?(\?MbX9?x&1? +?/$?Q?m?+?Zd;?7A`?On?Zd;O?Cl?L7A`??x&1?V-?7A`?#~j?B`"?-?ˡE?uV?V-?}?5^I?x?`"??5^I ?E?+?Zd;?= ףp=?NbX9?-?(\?/$?v?m?-?Y<Item Type="3" Title="Validation Expression">&lt;table border=1 cellspacing=0 cellpadding=0 style='border-collapse:collapse; border:none;mso-border-top-alt:solid black 1.5pt;mso-border-bottom-alt:solid black 1.5pt;mso-padding-alt:0pt 5.4pt 0pt 5.4pt'&gt;&lt;tr&gt;&lt;td&gt;Pos Control1&gt;2.0 - passed&lt;/td&gt;&lt;/tr&gt;&lt;/table&gt;</Item>PositivePositivePositivePositivePositivePositivePositivePositivePositiveNegativeNegativeNegativeNegativeNegativeNegativeNegativeNegativeNegativePositiveNegatived not evaluate: "w/w(0,1)" - The reference to well G3 is not allowed as that well has been flaggedUser&{A701F931-742E-4DA3-91F5-FCCA734D5DEA}@fCould not evaluate: "w/w(0,1)" - The reference to well G4 is not allowed as that well has been flaggedUser&{A701F931-742E-4DA3-91F5-FCCA734D5DEA}AfCould not evaluate: "w/w(0,1)" - The reference to well G5 is not allowed as that well has been flaggedUser&{A701F931-742E-4DA3-91F5-FCCA734D5DEA}BfCould not evaluate: "w/w(0,1)" - The reference to well G6 is not allowed as that well has been flaggedUser&{A701F931-742E-4DA3-91F5-FCCA734D5DEA}UDCould not evaluate: "w/w(0,1)" - Well offset out of microplate rangeUser&{A701F931-742E-4DA3-91F5-FCCA734D5DEA}VDCould not evaluate: "w/w(0,1)" - Well offset out of microplate rangeUser RawData*Ћ)Ћ)MeanDetailsTemplate+)Details,0-Ћ)Ћ)Readings./Ћ)P)TimeTemp4Ћ)Ћ)Details001Ћ)Ћ)AdminData32pWellsDataMeanData20nmRLU2 dV-?$@?Y@V-?$@`<table border="0" width=4(22 September 2004 16:17:14) Calculations, version 1Raw Data Group CalculationsPlate 10nmAnalysisTransform (1) IntS%@-g@= ףp=@-g~@S@-gn@(\@-g^@ʡE @-gN@{Gz@-g>@x&1 @-g.@sh|?@-g@ Zd;O @-g@!V- @-g@"Pn @-g@#333333 @3o@$Cl@3@%V- @3_@&}?5^I @3ױ@'Dl @3O@(h|?5 @3Dz@)On @3?@*ffffff@3@+On @3/@,x&1@3@-^I +@3@.Fx @3@/Cl@3@0Cl @3@1bX9@3@2K7@3w@3M@3@4K7@3g@5V-@3߸@6S@3W@7Dl@3Ϲ@8+@3G@9x@3@:y&1@37@;bX9@3@<x&@3'@=(\@3@>jt@3@? r@3@@"~@3@AB`"@3@B@3@CV-@3o@DK@3@EV-?l/@Fn@lk@G7A`?l@H|?5^@l@I r@l@J+?l[@KQ?l@L~jt?l@Msh|??l@NS㥛?lK@Ow/?l@PI +?l@Q/$?l@R`"?l;@S +?lw@TJ +?l@UM?l@VuV?l+@Wrh|?lg@X9v?l@Y~jt?l@Z/$?l@[^I +?lW@\p= ף?l@]Zd;?l@^S㥛?l @_?lG@`y&1?l@a'1Z?l@b/$?l@c7A`?l7@dd㥛 @Mb7@Mb?㥛 0@Mb?㥛 @On?y&1_@X9v?tn@Zd;?^I v@Q?^I w~@n?/$@B`"?/$@|?5^?/${@Zd;?/$;@  r?n@ $C?nݒ@ ףp= ?n@ $C?n@ S㥛?n}@= ףp=@n]@m@n=@Zd;O@n@1Zd @n@On @L7A@5^I @L7Aޡ@㥛 0@L7A΢@tV@L7Aྣ@x&@L7Aத@5^I @L7A@S@L7A@fffff@L7A~@Mb@L7An@(\@L7A^@-@L7AN@(\@L7A>@K7A@L7A.@NbX94@L7A@ ~jt@L7A@! rh@L7A@"V-@L7A@#Hz@ po@$m @ p@%sh|@ p_@&%C @ pױ@'xi@ pO@(㥛 @ pDz@)x& @ p?@*333333 @ p@+n @ p/@,K7@ p@-|?5^@ p@.n @ p@/$C @ p@09v @ p@1Mb@ p@2`dZd;@&1|7@h|?5?"~j@h|?5?Zd;@x&1?t_@bX9?^I n@Zd;?/$v@ףp= ?/$o~@Zd;?n@-?n@sh|??nw@Zd;?n7@ 7A`?L7A@ v/?L7Aے@ zG?L7A໔@ X9v?L7A@  rh?L7A{@Q@L7A[@/$@L7A;@x&1@L7A@ʡE@L7A@x&1 @ @tV @ ݡ@~jt @ ͢@Fx @ @Dl@ @`" @ @"~j@ @(\ @ }@5^I  @ m@ףp= @ ]@ʡE @ M@333333 @ =@x&1 @ -@ rh @ @ Pn@ @!K7 @ @"Zd;O @ @#zG @Mbn@$l @Mb@%L7A` @Mb^@& + @Mbֱ@'M @MbN@(I + @MbƲ@)Zd;O @Mb>@* @Mb@+x&1 @Mb.@,Zd;O @Mb@-+@Mb@.;On @Mb@/Cl@Mb@09v @Mb@1Dl @Mb@2jt@Mbv@3w/ @Mb@4tV@Mbf@5V-@Mb޸@6sh|? @MbV@7/$@Mbι@8sh|?@MbF@9n@Mb@:x&1@Mb6@;$C@Mb@<E@Mb&@=HzG@Mb@>jt@Mb@?^I +@Mb@@jt@Mb@A"~@Mb~@BGz@Mb@CL7A`@Mbn@D"~@Mb@E|?5^@&1|/@Fx&@&1|k@G r@&1|@H|?5^@&1|@I}?5^I@&1|@JX9v@&1|[@K?&1|@Lrh|@&1|@M|?5^?&1|@N/$@&1|K@OX9v?&1|@PMbX9@&1|@Q/$?&1|@RV-?&1|;@S rh?&1|w@T㥛 ?&1|@UMbX9?&1|@VK7?&1|+@WS?&1|g@XCl?&1|@Y"~j?&1|@ZX9v?&1|@[|?5^?&1|W@\)\(?&1|@]Zd;O?&1|@^-?&1| @_Q?&1|G@`Zd;?&1|@abX9?&1|@bʡE?&1|@cK7?&1|7@ddn@l7@bX9ȶ?{Gz@h|?5?n@bX9ȶ?E_@X9v?"~n@Zd;?h|?v@Q?h|?s~@x&1?v@`"?v@rh|?vy@E?v9@ K7A`?Zd;@ I +?Zd;ܒ@ {Gz?Zd;ϼ@  +?Zd;Ϝ@ ?5^I ?Zd;|@?Zd;\@ +?Zd;<@Fx@Zd;@ʡE@Zd;@ˡE@-g@K7A@-gޡ@V- @-g΢@7A`@-g@Mb@-g@V-@-g@- @ pw@3%C @ p@4B`" @ pg@5V-@ p߸@6sh|?@ pW@7Cl@ pϹ@8 r @ pG@9y&1@ p@:&1@ p7@;NbX9@ p@<x&1 @ p'@=333333 @ p@>K7A`@ p@?sh|?@ p@@K7@ p@AK7A`@ p@B@ p@C-@ po@D`"@ p@E +@Mb/@Fd;O@Mbk@G}?5^I@Mb@H5^I @Mb@I +@Mb@JDl@Mb[@KQ@Mb@L+@Mb@M|?5^?Mb@N/$@MbK@ObX9?Mb@PS㥛?Mb@Q/$?Mb@RV-?Mb;@S|?5^?Mbw@TJ +?Mb@UCl?Mb@V"~j?Mb+@WT㥛 ?Mbg@XDl?Mb@YV-?Mb@Z/$?Mb@[J +?MbW@\(\?Mb@]x&?Mb@^S㥛?Mb @_\(\?MbG@`l?Mb@ax&?Mb@bX9v?Mb@c#~j?Mb7@dd9v@u7@h|?5?= ףp@h|?5?9v@n?^I _@Mb?/$n@B`"?nv@(\?nz~@L7A`?L7A`@~jt?L7A`@/$?L7A`}@Zd;?L7A`=@  r? @ $C? ޒ@ (\? @ MbX? @ +? ~@? ^@l@ >@Zd; @ @Zd;O @ @jt@MbX@S%@MbXߡ@+N@MbXϢ@#~j@MbX@x@MbX@^I +@MbX@}?5^@MbX@= ףp@MbX@X9v@MbXo@Q@MbX_@"~j@MbXO@@MbX?@jt@MbX/@lq@MbX@ jt@MbX@!|?5^@MbX@"-@MbX@#ףp= W@&1o@$y&1,@&1@%jt@&1_@&Dl)@&1ױ@'x&1@&1O@(Mb@&1Dz@)d;O@&1?@*zG@&1@+x&@&1/@,7A`P@&1@-S㥛 @&1@.ˡE @&1@/l @&1@0Dl@&1@11Zd@&1@2`" @&1w@3Cl@&1@4 rh@&1g@5K7A` @&1߸@6 rh@&1W@7bX9 @&1Ϲ@8h|?5 @&1G@9n @&1@:1Zd @&17@;X9v @&1@<x&1 @&1'@=Q@&1@>L7A` @&1@?!rh@&1@@V-@&1@A~jt@&1@BRQ@&1@CV-@&1o@D-@&1@E}?5^I@u/@FZd;@uk@GS@u֧@HS㥛@u@I r@u@J$C@u[@K@u֗@LS@u@Mv/@u@Nv/@uK@O%C?uև@PS㥛?u@QMbX9?u@RV-?u;@S㥛 ?uw@TJ +?uֳ@U&1@u@VK7?u+@W!rh?ug@XMbX?u֣@Y"~?u@ZCl?u@[S?uW@\p= ף?u֓@]Pn?u@^ rh?u @_(\?uG@`ʡE?uփ@aʡE?uֿ@bX9v?u@cV-?u7@dd3Mb@7@MbX9?|?5^@MbX9?Mb@n?Dl_@x&?En@K7?"~v@(\?"~~~@V-?h|?@L7A`?h|?@sh|??h|?@y&1?h|??@ #~j?v@ MbX9?vߒ@ ?v@ 5^I ?v@ rh|?v@ ףp= ?v_@sh|??v?@ʡE@v@;On@v@ʡE@Zd;@-@Zd;ߡ@B`"@Zd;Ϣ@oʡ @Zd;Ͽ@X9v @Zd;ϯ@K7 @Zd;ϟ@V-@Zd;Ϗ@zG @Zd;@|?5^@Zd;o@q= ף@Zd;_@Fx@Zd;O@(\@Zd;?@uV@Zd;/@㥛 @Zd;@ tV@Zd;@!V- @Zd;@"-@Zd;@#RQ @-o@$d;O@-@%B`"@-_@&Clg@-ױ@'MbX9@-O@(S㥛 @-Dz@)A`"@-?@*@-緳@+V@-/@,-'@-秴@-/$@-@.ʡE@-痵@/Zd;O @-@0v/@-燶@1v@-@2|?5^@-w@3/$ @-@4|?5^@-g@5?5^I@-߸@6 +@-W@7/$@-Ϲ@8+ @-G@9Fx @-翺@:K@-7@;Cl @-篻@<Pn @-'@=q= ףp @-矼@>+N@-@? r@-珽@@`" @-@A`" @-@B)\(@-@CV-@-o@DK @-@E rh @/@FOn @k@G5^I  @@H}?5^I@@Irh| @@JCl@[@K@@L + @@MMb@@N/$@K@OPn@@P/$@@QZd;@@R^I +@;@SbX9@w@TX9v@@UʡE@@V|?5^@+@W/$@g@Xx&1@@Y!rh@@Zn@@[X9v@W@\RQ@@]#~j@@^S㥛? @_(\?G@`"~@@a;On?@bx&?@c|?5^?7@dd"@ @^I 8@J +?V-@J +?@ @'1Z?n`@x&?no@B`"?K7Aw@{Gz?K7A~@Zd;?㥛 @`"?㥛 @㥛 ?㥛 @Zd;O?㥛 A@  rh?Mb@ %C?Mb@ ffffff?Mb@ h|?5?Mb@ L7A`?Mb@(\?Mb`@J +?Mb@@1Zd@Mb @On@y&1H@ʡE@y&1H@B`"@y&1H@K7@y&1HТ@Zd;O@y&1H@l@y&1H@#~j @y&1H@- @y&1H@Q @y&1H@|?5^ @y&1Hp@ @y&1H`@d;O @y&1HP@HzG @y&1H@@ʡE @y&1H0@S @y&1H @ y&1@y&1H@!V-@y&1H@"Fx@y&1H@#Q @t$p@$v/ @t$@%uV @t$`@&J + @t$ر@'Mb @t$P@(!rh @t$Ȳ@)Pn @t$@@*zG@t$@+x&1 @t$0@,1Zd @t$@- r @t$ @.n@t$@//$ @t$@0$C@t$@1v/@t$@2tV@t$x@3NbX9@t$@4~jt @t$h@5K7@t$@6J + @t$X@7l@t$й@8S㥛@t$H@9d;O@t$@:On@t$8@;m@t$@<;On@t$(@=Q@t$@>#~j@t$@??5^I @t$@@V-@t$@Ajt@t$@BQ@t$@CV-@t$p@DuV@t$@E|?5^@^I 0@FMb?^I l@GI +@^I @H"~j?^I @IV-?^I  @J}?5^I?^I \@Kq= ףp?^I @L-?^I @M rh?^I @N|?5^?^I L@O$C?^I @P^I +?^I @Q/$?^I @RuV?^I <@SS?^I x@T^I +?^I @Uw/?^I @V~jt?^I ,@Wjt?^I h@Xv?^I @YK7?^I @Z/$?^I @[5^I ?^I X@\(\?^I @]ʡE?^I @^K7A`?^I  @_p= ף?^I H@`X9v?^I @aw/?^I @bDl?^I @c7A`?^I 8@dddZd;O!@uV8@V-?A`"?V-?Zd;O!@MbX?/$`@x&?/$o@Zd;?n w@zG?n~@-?L7A`@V-?L7A`ņ@5^I ?L7A`@X9v?L7A`E@ Pn? 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F@Zd;?MbX@/$?L7A`@tV?MbX@-?L7A`@ʡE?MbXӣ@/$?MbXä@ rh?MbX@uV?MbX@\(\?MbX@/$?MbX@Q?MbXs@ʡE?MbXc@Q?MbXS@oʡ?MbXC@MbX?MbX3@ K7?L7A`#@!S㥛?L7A`@"#~j?&1@#ffffff?&1y@$On?&1@%J +?&1i@&MbX?&1@'n?&1Y@(v?&1Ѳ@)7A`?&1I@*{Gz?&1@+jt?&19@,Zd;O?&1@-%C?&1)@."~j? @/d;O?&1@0Zd;? @1A`"? @2S㥛?&1@3V-?&1@45^I ?&1q@5S㥛?&1@6x&1?&1a@7-?&1ٹ@8Mb?&1Q@9-?&1ɺ@:?5^I ?&1A@;Zd;?&1@<-?&11@=Gz? @>!rh?&1!@?l?&1@@Cl?&1@A +?&1@BzG?&1@CM?&1y@Dw/?&1@EZd;?u4@F7A`?up@GX9v?u֬@HPn?u@Ix&1?u$@Jjt?Mb`@KQ?u֜@LMbX9?u@MZd;?u@Njt?uP@OV-?u֌@Pjt?u@Q+?u@R/$?u@@SʡE?u|@TV-?uָ@Ush|??u@Vx?u0@W-?ul@X#~j?Mbب@YZd;O?Mb@ZV-?u @[uV?u\@\Q?u֘@]w/?u@^x&1?u@_Q?uL@`MbX?uֈ@aCl?u@bMb?u@cx&?u<@dd[jt1@<@MbX9?+?ˡE?jt1@x?EMbX9?-!@?x&1?-白@@ +?-@A/$?-牾@BQ?-@Cm?-y@D+?-@EZd;?4@F7A`?p@GOn?@HZd;O?@ICl?$@JL7A`?u`@K?@Lx&1?@MV-?@N7A`?P@O#~j?@PB`"?@Q-?@RˡE?@@SuV?|@TV-?@U}?5^I?@Vx?0@W`"?l@X?5^I ?u@YE?u@Z+? @[Zd;?\@\= ףp=?@]NbX9?@^-?@_(\?L@`/$?@av?@bm?@c-?<@drc="\LOCALTEMPDIR\DAZC72.tmp"></td></tr></table><table border="0" width="0%"><tr><td><img src="\LOCALTEMPDIR\DAZC73.tmp"></td><td><img src="\LOCALTEMPDIR\DAZC74.tmp"></td></tr></table><table border="0" width="0%"><tr><td><img src="\LOCALTEMPDIR\DAZC75.tmp"></td><td><img src="\LOCALTEMPDIR\DAZC78.tmp"></td></tr></table><table border="0" width="0%"><tr><td><img src="\LOCALTEMPDIR\DAZC79.tmp"></td><td><img src="\LOCALTEMPDIR\DAZC7A.tmp"></td></tr></table>$<img src="\LOCALTEMPDIR\DAZC4A.tmp">$<img src="\LOCALTEMPDIR\DAZC4B.tmp">$<img src="\LOCALTEMPDIR\DAZC4C.tmp">$<img src="\LOCALTEMPDIR\DAZC4D.tmp">$<img src="\LOCALTEMPDIR\DAZC53.tmp">$<img src="\LOCALTEMPDIR\DAZC54.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC55.tmp">$<img src="\LOCALTEMPDIR\DAZC56.tmp">$<img src="\LOCALTEMPDIR\DAZC57.tmp">$<img src="\LOCALTEMPDIR\DAZC58.tmp">$<img src="\LOCALTEMPDIR\DAZC59.tmp">$<img src="\LOCALTEMPDIR\DAZC5A.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC5B.tmp">$<img src="\LOCALTEMPDIR\DAZC5C.tmp">$<img src="\LOCALTEMPDIR\DAZC5D.tmp">$<img src="\LOCALTEMPDIR\DAZC5E.tmp">$<img src="\LOCALTEMPDIR\DAZC5F.tmp">$<img src="\LOCALTEMPDIR\DAZC60.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">`<table border="0" width="0%"><tr><td><img src="\LOCALTEMPDIR\DAZC4A.tmp"></td><td><img src="\LOCALTEMPDIR\DAZC4B.tmp"></td></tr></table><table border="0" width="0%"><tr><td><img src="\LOCALTEMPDIR\DAZC4C.tmp"></td><td><img src="\LOCALTEMPDIR\DAZC4D.tmp"></td></tr></table><table border="0" width="0%"><tr><td><img src="\LOCALTEMPDIR\DAZC53.tmp"></td><td><img src="\LOCALTEMPDIR\DAZC54.tmp"></td></tr></table><table border="0" width="0%"><tr><td><img src="\LOCALTEMPDIR\DAZC5B.tmp"></td><td><img src="\LOCALTEMPDIR\DAZC5C.tmp"></td></tr></table><table border="0" width="0%"><tr><td><img src="\LOCALTEMPDIR\DAZC5D.tmp"></td><td><img src="\LOCALTEMPDIR\DAZC5E.tmp"></td></tr></table><table border="0" width="0%"><tr><td><img src="\LOCALTEMPDIR\DAZC5F.tmp"></td><td><img src="\LOCALTEMPDIR\DAZC60.tmp"></td></tr></table><table border="0" width="0%"><tr><td><img src="\LOCALTEMPDIR\DAZC69.tmp"></td><td><img src="\LOCALTEMPDIR\DAZC6A.tmp"></td></tr></table><table border="0" width="0%"><tr><td><img src="\LOCALTEMPDIR\DAZC6B.tmp"></td><td><img src="\LOCALTEMPDIR\DAZC6C.tmp"></td></tr></table><table border="0" width="0%"><tr><td><img src="\LOCALTEMPDIR\DAZC6D.tmp"></td><td><img src="\LOCALTEMPDIR\DAZC6E.tmp"></td></tr></table><table border="0" width="0%"><tr><td><img src="\LOCALTEMPDIR\DAZC55.tmp"></td><td><img src="\LOCALTEMPDIR\DAZC56.tmp"></td></tr></table><table border="0" width="0%"><tr><td><img src="\LOCALTEMPDIR\DAZC57.tmp"></td><td><img src="\LOCALTEMPDIR\DAZC58.tmp"></td></tr></table><table border="0" width="0%"><tr><td><img src="\LOCALTEMPDIR\DAZC59.tmp"></td><td><img src="\LOCALTEMPDIR\DAZC5A.tmp"></td></tr></table><table border="0" width="0%"><tr><td><img src="\LOCALTEMPDIR\DAZC61.tmp"></td><td><img src="\LOCALTEMPDIR\DAZC62.tmp"></td></tr></table><table border="0" width="0%"><tr><td><img src="\LOCALTEMPDIR\DAZC63.tmp"></td><td><img src="\LOCALTEMPDIR\DAZC66.tmp"></td></tr></table><table border="0" width="0%"><tr><td><img src="\LOCALTEMPDIR\DAZC67.tmp"></td><td><img src="\LOCALTEMPDIR\DAZC68.tmp"></td></tr></table><table border="0" width="0%"><tr><td><img src="\LOCALTEMPDIR\DAZC6F.tmp"></td><td><img src="\LOCALTEMPDIR\DAZC70.tmp"></td></tr></table><table border="0" width="0%"><tr><td><img src="\LOCALTEMPDIR\DAZC71.tmp"></td><td><img s$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC61.tmp">$<img src="\LOCALTEMPDIR\DAZC62.tmp">$<img src="\LOCALTEMPDIR\DAZC63.tmp">$<img src="\LOCALTEMPDIR\DAZC66.tmp">$<img src="\LOCALTEMPDIR\DAZC67.tmp">$<img src="\LOCALTEMPDIR\DAZC68.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC69.tmp">$<img src="\LOCALTEMPDIR\DAZC6A.tmp">$<img src="\LOCALTEMPDIR\DAZC6B.tmp">$<img src="\LOCALTEMPDIR\DAZC6C.tmp">$<img src="\LOCALTEMPDIR\DAZC6D.tmp">$<img src="\LOCALTEMPDIR\DAZC6E.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC6F.tmp">$<img src="\LOCALTEMPDIR\DAZC70.tmp">$<img src="\LOCALTEMPDIR\DAZC71.tmp">$<img src="\LOCALTEMPDIR\DAZC72.tmp">$<img src="\LOCALTEMPDIR\DAZC73.tmp">$<img src="\LOCALTEMPDIR\DAZC74.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC75.tmp">$<img src="\LOCALTEMPDIR\DAZC78.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC79.tmp">$<img src="\LOCALTEMPDIR\DAZC7A.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">$<img src="\LOCALTEMPDIR\DAZC49.tmp">DetailsAuditTrailWpezium method; Result = 68.5255ID4 (Standard5) Integral calculated by Trapezium method; Result = 64.7005ID5 (Standard6) Integral calculated by Trapezium method; Result = 69.4555ID6 (Standard6) Integral calculated by Trapezium method; Result = 64.0005ME1 (D113 v 7579696) Integral calculated by Trapezium method; Result = 277.51NE2 (D113 v 7579696) Integral calculated by Trapezium method; Result = 330.465LE3 (D398 v 7579696) Integral calculated by Trapezium method; Result = 248.5NE4 (D398 v 7579696) Integral calculated by Trapezium method; Result = 135.645ME5 (D77 v 7579696) Integral calculated by Trapezium method; Result = 160.276LE6 (D77 v 7579696) Integral calculated by Trapezium method; Result = 249.31IF1 (Standard7) Integral calculated by Trapezium method; Result = 39.1805IF2 (Standard7) Integral calculated by Trapezium method; Result = 48.6755IF3 (Standard8) Integral calculated by Trapezium method; Result = 54.8955IF4 (Standard8) Integral calculated by Trapezium method; Result = 52.7005IF5 (Standard9) Integral calculated by Trapezium method; Result = 48.5605IF6 (Standard9) Integral calculated by Trapezium method; Result = 49.7455KG1 (Pos Control1) Integral calculated by Trapezium method; Result = 189.43LG2 (Pos Control1) Integral calculated by Trapezium method; Result = 221.241LH1 (Neg Control1) Integral calculated by Trapezium method; Result = 38.3605LH2 (Neg Control1) Integral calculated by Trapezium method; Result = 44.4505Group CalculationsIntegralD133 v 757973E A1 = 232.565 A2 = 215.465Average = 448.031 / 2 = 224.015D398 v 757973E A3 = 262.646 A4 = 272.436Average = 535.081 / 2 = 267.541 D77 v 757973E A5 = 287.541 A6 = 207.101Average = 494.641 / 2 = 247.321D113 v 720281J C1 = 239.041 C2 = 213.441Average = 452.481 / 2 = 226.241D398 v 720281J C3 = 201.816 C4 = 188.5814(22 September 2004 16:17:14) Calculations, version 1Raw Data Group CalculationsPlate 10nmAnalysisTransform (1) IntegralDetailsModule-C:\Program Files\Dazdaq\Manta\KRTransform.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 680,024 bytes Created = 25 June 2004, 12:01:14 CalculationseIntegrated area by trapezium method. Using all unflagged data points, Axes = (Cycle Number, Reading).Input Matrices 0nm (RLU) Output MatricesIntegralDetailsPlate 1 Calculations)NA1 (D133 v 757973E) Integral calculated by Trapezium method; Result = 232.565NA2 (D133 v 757973E) Integral calculated by Trapezium method; Result = 215.465NA3 (D398 v 757973E) Integral calculated by Trapezium method; Result = 262.646NA4 (D398 v 757973E) Integral calculated by Trapezium method; Result = 272.436MA5 (D77 v 757973E) Integral calculated by Trapezium method; Result = 287.541MA6 (D77 v 757973E) Integral calculated by Trapezium method; Result = 207.101IB1 (Standard1) Integral calculated by Trapezium method; Result = 36.5455IB2 (Standard1) Integral calculated by Trapezium method; Result = 35.7005IB3 (Standard2) Integral calculated by Trapezium method; Result = 41.3855IB4 (Standard2) Integral calculated by Trapezium method; Result = 39.8005IB5 (Standard3) Integral calculated by Trapezium method; Result = 41.4255IB6 (Standard3) Integral calculated by Trapezium method; Result = 54.4355NC1 (D113 v 720281J) Integral calculated by Trapezium method; Result = 239.041NC2 (D113 v 720281J) Integral calculated by Trapezium method; Result = 213.441NC3 (D398 v 720281J) Integral calculated by Trapezium method; Result = 201.816NC4 (D398 v 720281J) Integral calculated by Trapezium method; Result = 188.581LC5 (D77 v 720281J) Integral calculated by Trapezium method; Result = 265.72MC6 (D77 v 720281J) Integral calculated by Trapezium method; Result = 325.116ID1 (Standard4) Integral calculated by Trapezium method; Result = 161.821ID2 (Standard4) Integral calculated by Trapezium method; Result = 57.7105ID3 (Standard5) Integral calculated by TraAverage = 390.396 / 2 = 195.198 D77 v 720281J C5 = 265.72 C6 = 325.116Average = 590.836 / 2 = 295.418D113 v 7579696 E1 = 277.51 E2 = 330.465Average = 607.976 / 2 = 303.988D398 v 7579696 E3 = 248.5 E4 = 135.645Average = 384.146 / 2 = 192.073 D77 v 7579696 E5 = 160.276 E6 = 249.31Average = 409.586 / 2 = 204.793 Standard1 B1 = 36.5455 B2 = 35.7005Average = 72.246 / 2 = 36.123 Standard2 B3 = 41.3855 B4 = 39.8005Average = 81.186 / 2 = 40.593 Standard3 B5 = 41.4255 B6 = 54.4355Average = 95.861 / 2 = 47.9305 Standard4 D1 = 161.821 D2 = 57.7105Average = 219.531 / 2 = 109.766 Standard5 D3 = 68.5255 D4 = 64.7005Average = 133.226 / 2 = 66.613 Standard6 D5 = 69.4555 D6 = 64.0005Average = 133.456 / 2 = 66.728 Standard7 F1 = 39.1805 F2 = 48.6755Average = 87.856 / 2 = 43.928 Standard8 F3 = 54.8955 F4 = 52.7005Average = 107.596 / 2 = 53.798 Standard9 F5 = 48.5605 F6 = 49.7455Average = 98.306 / 2 = 49.153 Pos Control1 G1 = 189.43 G2 = 221.241Average = 410.671 / 2 = 205.335 Neg Control1 H1 = 38.3605 H2 = 44.4505Average = 82.811 / 2 = 41.4055Transform (2) Matrix ExpressionDetailsModule,C:\Program Files\Dazdaq\Manta\Transforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 888,918 bytes Created = 25 June 2004, 12:01:04 Calculations "w/w(0,3)"Input Matrices Integral () Output Matrices Opsonic IndexNot CalculatedB1 (Standard1) B2 (Standard1) B3 (Standard2) B4 (Standard2) B5 (Standard3) B6 (Standard3) D1 (Standard4) D2 (Standard4) D3 (Standard5) D4 (Standard5) D5 (Standard6) D6 (Standard6) F1 (Standard7) F2 (Standard7) F3 (Standard8) F4 (Standard8) F5 (Standard9) F6 (Standard9) DetailsPlate 1 Calculations*A1 (D133 v 757973E) = w/w(0,3) = 1.43718*A2 (D133 v 757973E) = w/w(0,3) = 3.73356*A3 (D398 v 757973E) = w/w(0,3) = 3.83281*A4 (D398 v 757973E) = w/w(0,3) = 4.21072)A5 (D77 v 757973E) = w/w(0,3) = 4.13992)A6 (D77 v 757973E) = w/w(0,3) = 3.23592*C1 (D113 v 720281J) = w/w(0,3) = 6.10101*C2 (D113 v 720281J) = w/w(0,3) = 4.38497*C3 (D398 v 720281J) = w/w(0,3) = 3.67636*C4 (D398 v 720281J) = w/w(0,3) = 3.57834)C5 (D77 v 720281J) = w/w(0,3) = 5.47195)C6 (D77 v 720281J) = w/w(0,3) = 6.53558*E1 (D113 v 7579696) = w/w(0,3) = 7.23428*E2 (D113 v 7579696) = w/w(0,3) = 7.43446{E3 (D398 v 7579696) Could not evaluate: "w/w(0,3)" - The reference to well H3 is not allowed as that well has been flagged{E4 (D398 v 7579696) Could not evaluate: "w/w(0,3)" - The reference to well H4 is not allowed as that well has been flaggedzE5 (D77 v 7579696) Could not evaluate: "w/w(0,3)" - The reference to well H5 is not allowed as that well has been flaggedzE6 (D77 v 7579696) Could not evaluate: "w/w(0,3)" - The reference to well H6 is not allowed as that well has been flaggedWG1 (Pos Control1) Could not evaluate: "w/w(0,3)" - Well offset out of microplate rangeWG2 (Pos Control1) Could not evaluate: "w/w(0,3)" - Well offset out of microplate rangeWH1 (Neg Control1) Could not evaluate: "w/w(0,3)" - Well offset out of microplate rangeWH2 (Neg Control1) Could not evaluate: "w/w(0,3)" - Well offset out of microplate rangeGroup Calculations Opsonic IndexD133 v 757973E A1 = 1.43718 A2 = 3.73356Average = 5.17074 / 2 = 2.58537D398 v 757973E A3 = 3.83281 A4 = 4.21072Average = 8.04353 / 2 = 4.02177 D77 v 757973E A5 = 4.13992 A6 = 3.23592Average = 7.37584 / 2 = 3.68792D113 v 720281J C1 = 6.10101 C2 = 4.38497Average = 10.486 / 2 = 5.24299D398 v 720281J C3 = 3.67636 C4 = 3.57834Average = 7.2547 / 2 = 3.62735 D77 v 720281J C5 = 5.47195 C6 = 6.53558Average = 12.0075 / 2 = 6.00376D113 v 7579696 E1 = 7.23428 E2 = 7.43446Average = 14.6687 / 2 = 7.33437D398 v 7579696&E3 = NOT INCLUDED - calculation error&E4 = NOT INCLUDED - calculation error&All wells in this group were excluded. D77 v 7579696&E5 = NOT INCLUDED - calculation error&E6 = NOT INCLUDED - calculation error&All wells in this group were excluded. Standard1)B1 = NOT INCLUDED - no result calculated)B2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard2)B3 = NOT INCLUDED - no result calculated)B4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard3)B5 = NOT INCLUDED - no result calculated)B6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard4)D1 = NOT INCLUDED - no result calculated)D2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard5)D3 = NOT INCLUDED - no result calculated)D4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard6)D5 = NOT INCLUDED - no result calculated)D6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard7)F1 = NOT INCLUDED - no result calculated)F2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard8)F3 = NOT INCLUDED - no result calculated)F4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard9)F5 = NOT INCLUDED - no result calculated)F6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Pos Control1&G1 = NOT INCLUDED - calculation error&G2 = NOT INCLUDED - calculation error&All wells in this group were excluded. Neg Control1&H1 = NOT INCLUDED - calculation error&H2 = NOT INCLUDED - calculation error&All wells in this group were excluded.Transform (3) Auto Flag By WellDetailsModule,C:\Program Files\Dazdaq\Manta\Transforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 8      !"#$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcdefghijklmnopqrstuvwxyz{|}~88,918 bytes Created = 25 June 2004, 12:01:04 Calculationsx>1.2Input MatricesOpsonic Index () Not CalculatedB1 (Standard1) B2 (Standard1) B3 (Standard2) B4 (Standard2) B5 (Standard3) B6 (Standard3) D1 (Standard4) D2 (Standard4) D3 (Standard5) D4 (Standard5) D5 (Standard6) D6 (Standard6) F1 (Standard7) F2 (Standard7) F3 (Standard8) F4 (Standard8) F5 (Standard9) F6 (Standard9) G1 (Pos Control1) G2 (Pos Control1) H1 (Neg Control1) H2 (Neg Control1) DetailsPlate 1 CalculationsExpression = x>1.2BA1 (D133 v 757973E) x = 1.43718, Result = TRUE, Well AUTO-FLAGGEDBA2 (D133 v 757973E) x = 3.73356, Result = TRUE, Well AUTO-FLAGGEDBA3 (D398 v 757973E) x = 3.83281, Result = TRUE, Well AUTO-FLAGGEDBA4 (D398 v 757973E) x = 4.21072, Result = TRUE, Well AUTO-FLAGGEDAA5 (D77 v 757973E) x = 4.13992, Result = TRUE, Well AUTO-FLAGGEDAA6 (D77 v 757973E) x = 3.23592, Result = TRUE, Well AUTO-FLAGGEDBC1 (D113 v 720281J) x = 6.10101, Result = TRUE, Well AUTO-FLAGGEDBC2 (D113 v 720281J) x = 4.38497, Result = TRUE, Well AUTO-FLAGGEDBC3 (D398 v 720281J) x = 3.67636, Result = TRUE, Well AUTO-FLAGGEDBC4 (D398 v 720281J) x = 3.57834, Result = TRUE, Well AUTO-FLAGGEDAC5 (D77 v 720281J) x = 5.47195, Result = TRUE, Well AUTO-FLAGGEDAC6 (D77 v 720281J) x = 6.53558, Result = TRUE, Well AUTO-FLAGGEDBE1 (D113 v 7579696) x = 7.23428, Result = TRUE, Well AUTO-FLAGGEDBE2 (D113 v 7579696) x = 7.43446, Result = TRUE, Well AUTO-FLAGGED_E3 (D398 v 7579696) The data point E3 (D398 v 7579696) is already flagged (calculation error)_E4 (D398 v 7579696) The data point E4 (D398 v 7579696) is already flagged (calculation error)]E5 (D77 v 7579696) The data point E5 (D77 v 7579696) is already flagged (calculation error)]E6 (D77 v 7579696) The data point E6 (D77 v 7579696) is already flagged (calculation error)Transform (4) Matrix ExpressionDetailsModule,C:\Program Files\Dazdaq\Manta\Transforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 888,918 bytes Created = 25 June 2004, 12:01:04 Calculations"G1/G2"Input Matrices Integral () Output Matrices Control OINot Calculated&A1 (D133 v 757973E) A2 (D133 v 757973E) A3 (D398 v 757973E) A4 (D398 v 757973E) A5 (D77 v 757973E) A6 (D77 v 757973E) B1 (Standard1) B2 (Standard1) B3 (Standard2) B4 (Standard2) B5 (Standard3) B6 (Standard3) C1 (D113 v 720281J) C2 (D113 v 720281J) C3 (D398 v 720281J) C4 (D398 v 720281J) C5 (D77 v 720281J) C6 (D77 v 720281J) D1 (Standard4) D2 (Standard4) D3 (Standard5) D4 (Standard5) D5 (Standard6) D6 (Standard6) E1 (D113 v 7579696) E2 (D113 v 7579696) E3 (D398 v 7579696) E4 (D398 v 7579696) E5 (D77 v 7579696) E6 (D77 v 7579696) F1 (Standard7) F2 (Standard7) F3 (Standard8) F4 (Standard8) F5 (Standard9) F6 (Standard9) H1 (Neg Control1) H2 (Neg Control1) DetailsPlate 1 Calculations%G1 (Pos Control1) = G1/G2 = 0.85622%G2 (Pos Control1) = G1/G2 = 0.85622Group Calculations Control OID133 v 757973E)A1 = NOT INCLUDED - no result calculated)A2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D398 v 757973E)A3 = NOT INCLUDED - no result calculated)A4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. D77 v 757973E)A5 = NOT INCLUDED - no result calculated)A6 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D113 v 720281J)C1 = NOT INCLUDED - no result calculated)C2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D398 v 720281J)C3 = NOT INCLUDED - no result calculated)C4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. D77 v 720281J)C5 = NOT INCLUDED - no result calculated)C6 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D113 v 7579696)E1 = NOT INCLUDED - no result calculated)E2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D398 v 7579696)E3 = NOT INCLUDED - no result calculated)E4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. D77 v 7579696)E5 = NOT INCLUDED - no result calculated)E6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard1)B1 = NOT INCLUDED - no result calculated)B2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard2)B3 = NOT INCLUDED - no result calculated)B4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard3)B5 = NOT INCLUDED - no result calculated)B6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard4)D1 = NOT INCLUDED - no result calculated)D2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard5)D3 = NOT INCLUDED - no result calculated)D4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard6)D5 = NOT INCLUDED - no result calculated)D6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard7)F1 = NOT INCLUDED - no result calculated)F2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard8)F3 = NOT INCLUDED - no result calculated)F4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard9)F5 = NOT INCLUDED - no result calculated)F6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Pos Control1 G1 = 0.85622 G2 = 0.85622Average = 1.71244 / 2 = 0.85622 Neg Control1)H1 = NOT INCLUDED - no result calculated)H2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.#Transform (5) Validation ExpressionDetailsModule-C:\Program Files\Dazdaq\Manta\QTransforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 610,392 bytes Created = 25 June 2004, 12:01:40 CalculationsValidates: x>2.0Input MatricesControl OI () DetailsPlate 1 Calculations\Could not evaluate expression: "x>2.0" - x cannot be referenced as it has not been specified(22 September 2004 16:17:18) Saved As C:\Documents and Settings\LyP\Personal\My Assays\Results\Slow Kinetics\Slow Kinetics (1).ars(13 October 2004 09:57:00) Accessing C:\Documents and Settings\LyP\Personal\My Assays\Results\Slow Kinetics\Slow Kinetics (1).ars2(13 October 2004 09:58:07) Calculations, version 2Raw Data Group CalculationsPlate 10nmAnalysisTransform (1) IntegralDetailsModule-C:\Program Files\Dazdaq\Manta\KRTransform.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 680,024 bytes Created = 25 June 2004, 12:01:14 CalculationseIntegrated area by trapezium method. Using all unflagged data points, Axes = (Cycle Number, Reading).Input Matrices 0nm (RLU) Output MatricesIntegralDetailsPlate 1 Calculations)NA1 (D133 v 757973E) Integral calculated by Trapezium method; Result = 232.565NA2 (D133 v 757973E) Integral calculated by Trapezium method; Result = 215.465NA3 (D398 v 757973E) Integral calculated by Trapezium method; Result = 262.646NA4 (D398 v 757973E) Integral calculated by Trapezium method; Result = 272.435MA5 (D77 v 757973E) Integral calculated by Trapezium method; Result = 287.541MA6 (D77 v 757973E) Integral calculated by Trapezium method; Result = 207.101IB1 (Standard1) Integral calculated by Trapezium method; Result = 36.5455IB2 (Standard1) Integral calculated by Trapezium method; Result = 35.7005IB3 (Standard2) Integral calculated by Trapezium method; Result = 41.3855IB4 (Standard2) Integral calculated by Trapezium method; Result = 39.8005IB5 (Standard3) Integral calculated by Trapezium method; Result = 41.4255IB6 (Standard3) Integral calculated by Trapezium method; Result = 54.4355NC1 (D113 v 720281J) Integral calculated by Trapezium method; Result = 239.041MC2 (D113 v 720281J) Integral calculated by Trapezium method; Result = 213.44NC3 (D398 v 720281J) Integral calculated by Trapezium method; Result = 201.816NC4 (D398 v 720281J) Integral calculated by Trapezium method; Result = 188.581MC5 (D77 v 720281J) Integral calculated by Trapezium method; Result = 265.721MC6 (D77 v 720281J) Integral calculated by Trapezium method; Result = 325.116ID1 (Standard4) Integral calculated by Trapezium method; Result = 161.821ID2 (Standard4) Integral calculated by Trapezium method; Result = 57.7105ID3 (Standard5) Integral calculated by Trapezium method; Result = 68.5255ID4 (Standard5) Integral calculated by Trapezium method; Result = 64.7005ID5 (Standard6) Integral calculated by Trapezium method; Result = 69.4555ID6 (Standard6) Integral calculated by Trapezium method; Result = 64.0005ME1 (D113 v 7579696) Integral calculated by Trapezium method; Result = 277.51NE2 (D113 v 7579696) Integral calculated by Trapezium method; Result = 330.466LE3 (D398 v 7579696) Integral calculated by Trapezium method; Result = 248.5NE4 (D398 v 7579696) Integral calculated by Trapezium method; Result = 135.646ME5 (D77 v 7579696) Integral calculated by Trapezium method; Result = 160.275LE6 (D77 v 7579696) Integral calculated by Trapezium method; Result = 249.31IF1 (Standard7) Integral calculated by Trapezium method; Result = 39.1805IF2 (Standard7) Integral calculated by Trapezium method; Result = 48.6755IF3 (Standard8) Integral calculated by Trapezium method; Result = 54.8955IF4 (Standard8) Integral calculated by Trapezium method; Result = 52.7005IF5 (Standard9) Integral calculated by Trapezium method; Result = 48.5605IF6 (Standard9) Integral calculated by Trapezium method; Result = 49.7455KG1 (Pos Control1) Integral calculated by Trapezium method; Result = 189.43LG2 (Pos Control1) Integral calculated by Trapezium method; Result = 221.241LH1 (Neg Control1) Integral calculated by Trapezium method; Result = 38.3605LH2 (Neg Control1) Integral calculated by Trapezium method; Result = 44.4505Group CalculationsIntegralD133 v 757973E A1 = 232.565 A2 = 215.465Average = 448.031 / 2 = 224.015D398 v 757973E A3 = 262.646 A4 = 272.435Average = 535.081 / 2 = 267.541 D77 v 757973E A5 = 287.541 A6 = 207.101Average = 494.641 / 2 = 247.321D113 v 720281J C1 = 239.041 C2 = 213.44Average = 452.481 / 2 = 226.241D398 v 720281J C3 = 201.816 C4 = 188.581Average = 390.396 / 2 = 195.198 D77 v 720281J C5 = 265.721 C6 = 325.116Average = 590.836 / 2 = 295.418D113 v 7579696 E1 = 277.51 E2 = 330.466Average = 607.976 / 2 = 303.988D398 v 7579696 E3 = 248.5 E4 = 135.646Average = 384.146 / 2 = 192.073 D77 v 7579696 E5 = 160.275 E6 = 249.31Average = 409.586 / 2 = 204.793 Standard1 B1 = 36.5455 B2 = 35.7005Average = 72.246 / 2 = 36.123 Standard2 B3 = 41.3855 B4 = 39.8005Average = 81.186 / 2 = 40.593 Standard3 B5 = 41.4255 B6 = 54.4355Average = 95.861 / 2 = 47.9305 Standard4 D1 = 161.821 D2 = 57.7105Average = 219.531 / 2 = 109.766 Standard5 D3 = 68.5255 D4 = 64.7005Average = 133.226 / 2 = 66.613 Standard6 D5 = 69.4555 D6 = 64.0005Average = 133.456 / 2 = 66.728 Standard7 F1 = 39.1805 F2 = 48.6755Average = 87.856 / 2 = 43.928 Standard8 F3 = 54.8955 F4 = 52.7005Average = 107.596 / 2 = 53.798 Standard9 F5 = 48.5605 F6 = 49.7455Average = 98.306 / 2 = 49.153 Pos Control1 G1 = 189.43 G2 = 221.241Average = 410.671 / 2 = 205.336 Neg Control1 H1 = 38.3605 H2 = 44.4505Average = 82.811 / 2 = 41.4055Transform (2) Matrix ExpressionDetailsModule,C:\Program Files\Dazdaq\Manta\Transforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 888,918 bytes Created = 25 June 2004, 12:01:04 Calculations "w/w(0,1)"Input Matrices Integral () Output Matrices Opsonic IndexNot CalculatedB1 (Standard1) B2 (Standard1) B3 (Standard2) B4 (Standard2) B5 (Standard3) B6 (Standard3) D1 (Standard4) D2 (Standard4) D3 (Standard5) D4 (Standard5) D5 (Standard6) D6 (Standard6) F1 (Standard7) F2 (Standard7) F3 (Standard8) F4 (Standard8) F5 (Standard9) F6 (Standard9) DetailsPlate 1 Calculations*A1 (D133 v 757973E) = w/w(0,1) = 6.36372*A2 (D133 v 757973E) = w/w(0,1) = 6.03536*A3 (D398 v 757973E) = w/w(0,1) = 6.34632*A4 (D398 v 757973E) = w/w(0,1) = 6.84503)A5 (D77 v 757973E) = w/w(0,1) = 6.94115)A6 (D77 v 757973E) = w/w(0,1) = 3.80451)C1 (D113 v 720281J) = w/w(0,1) = 1.4772*C2 (D113 v 720281J) = w/w(0,1) = 3.69847*C3 (D398 v 720281J) = w/w(0,1) = 2.94512*C4 (D398 v 720281J) = w/w(0,1) = 2.91467)C5 (D77 v 720281J) = w/w(0,1) = 3.82577)C6 (D77 v 720281J) = w/w(0,1) = 5.07989*E1 (D113 v 7579696) = w/w(0,1) = 7.08287*E2 (D113 v 7579696) = w/w(0,1) = 6.78915*E3 (D398 v 7579696) = w/w(0,1) = 4.52679*E4 (D398 v 7579696) = w/w(0,1) = 2.57389)E5 (D77 v 7579696) = w/w(0,1) = 3.30053)E6 (D77 v 7579696) = w/w(0,1) = 5.01172(G1 (Pos Control1) = w/w(0,1) = 4.93817(G2 (Pos Control1) = w/w(0,1) = 4.97723WH1 (Neg Control1) Could not evaluate: "w/w(0,1)" - Well offset out of microplate rangeWH2 (Neg Control1) Could not evaluate: "w/w(0,1)" - Well offset out of microplate rangeGroup Calculations Opsonic IndexD133 v 757973E A1 = 6.36372 A2 = 6.03536Average = 12.3991 / 2 = 6.19954D398 v 757973E A3 = 6.34632 A4 = 6.84503Average = 13.1913 / 2 = 6.59567 D77 v 757973E A5 = 6.94115 A6 = 3.80451Average = 10.7457 / 2 = 5.37283D113 v 720281J C1 = 1.4772 C2 = 3.69847Average = 5.17566 / 2 = 2.58783D398 v 720281J C3 = 2.94512 C4 = 2.91467Average = 5.85978 / 2 = 2.92989 D77 v 720281J C5 = 3.82577 C6 = 5.07989Average = 8.90566 / 2 = 4.45283D113 v 7579696 E1 = 7.08287 E2 = 6.78915Average = 13.872 / 2 = 6.93601D398 v 7579696 E3 = 4.52679 E4 = 2.57389Average = 7.10069 / 2 = 3.55034 D77 v 7579696 E5 = 3.30053 E6 = 5.01172Average = 8.31225 / 2 = 4.15613 Standard1)B1 = NOT INCLUDED - no result calculated)B2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard2)B3 = NOT INCLUDED - no result calculated)B4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard3)B5 = NOT INCLUDED - no result calculated)B6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard4)D1 = NOT INCLUDED - no result calculated)D2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard5)D3 = NOT INCLUDED - no result calculated)D4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard6)D5 = NOT INCLUDED - no result calculated)D6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard7)F1 = NOT INCLUDED - no result calculated)F2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard8)F3 = NOT INCLUDED - no result calculated)F4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard9)F5 = NOT INCLUDED - no result calculated)F6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Pos Control1 G1 = 4.93817 G2 = 4.97723Average = 9.9154 / 2 = 4.9577 Neg Control1&H1 = NOT INCLUDED - calculation error&H2 = NOT INCLUDED - calculation error&All wells in this group were excluded.Transform (3) Auto Flag By WellDetailsModule,C:\Program Files\Dazdaq\Manta\Transforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 888,918 bytes Created = 25 June 2004, 12:01:04 Calculationsx>1.2Input MatricesOpsonic Index () Not CalculatedB1 (Standard1) B2 (Standard1) B3 (Standard2) B4 (Standard2) B5 (Standard3) B6 (Standard3) D1 (Standard4) D2 (Standard4) D3 (Standard5) D4 (Standard5) D5 (Standard6) D6 (Standard6) F1 (Standard7) F2 (Standard7) F3 (Standard8) F4 (Standard8) F5 (Standard9) F6 (Standard9) G1 (Pos Control1) G2 (Pos Control1) H1 (Neg Control1) H2 (Neg Control1) DetailsPlate 1 CalculationsExpression = x>1.2BA1 (D133 v 757973E) x = 6.36372, Result = TRUE, Well AUTO-FLAGGEDBA2 (D133 v 757973E) x = 6.03536, Result = TRUE, Well AUTO-FLAGGEDBA3 (D398 v 757973E) x = 6.34632, Result = TRUE, Well AUTO-FLAGGEDBA4 (D398 v 757973E) x = 6.84503, Result = TRUE, Well AUTO-FLAGGEDAA5 (D77 v 757973E) x = 6.94115, Result = TRUE, Well AUTO-FLAGGEDAA6 (D77 v 757973E) x = 3.80451, Result = TRUE, Well AUTO-FLAGGEDAC1 (D113 v 720281J) x = 1.4772, Result = TRUE, Well AUTO-FLAGGEDBC2 (D113 v 720281J) x = 3.69847, Result = TRUE, Well AUTO-FLAGGEDBC3 (D398 v 720281J) x = 2.94512, Result = TRUE, Well AUTO-FLAGGEDBC4 (D398 v 720281J) x = 2.91467, Result = TRUE, Well AUTO-FLAGGEDAC5 (D77 v 720281J) x = 3.82577, Result = TRUE, Well AUTO-FLAGGEDAC6 (D77 v 720281J) x = 5.07989, Result = TRUE, Well AUTO-FLAGGEDBE1 (D113 v 7579696) x = 7.08287, Result = TRUE, Well AUTO-FLAGGEDBE2 (D113 v 7579696) x = 6.78915, Result = TRUE, Well AUTO-FLAGGEDBE3 (D398 v 7579696) x = 4.52679, Result = TRUE, Well AUTO-FLAGGEDBE4 (D398 v 7579696) x = 2.57389, Result = TRUE, Well AUTO-FLAGGEDAE5 (D77 v 7579696) x = 3.30053, Result = TRUE, Well AUTO-FLAGGEDAE6 (D77 v 7579696) x = 5.01172, Result = TRUE, Well AUTO-FLAGGEDTransform (4) Matrix ExpressionDetailsModule,C:\Program Files\Dazdaq\Manta\Transforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 888,918 bytes Created = 25 June 2004, 12:01:04 Calculations"G1/G2"Input Matrices Integral () Output Matrices Control OINot Calculated&A1 (D133 v 757973E) A2 (D133 v 757973E) A3 (D398 v 757973E) A4 (D398 v 757973E) A5 (D77 v 757973E) A6 (D77 v 757973E) B1 (Standard1) B2 (Standard1) B3 (Standard2) B4 (Standard2) B5 (Standard3) B6 (Standard3) C1 (D113 v 720281J) C2 (D113 v 720281J) C3 (D398 v 720281J) C4 (D398 v 720281J) C5 (D77 v 720281J) C6 (D77 v 720281J) D1 (Standard4) D2 (Standard4) D3 (Standard5) D4 (Standard5) D5 (Standard6) D6 (Standard6) E1 (D113 v 7579696) E2 (D113 v 7579696) E3 (D398 v 7579696) E4 (D398 v 7579696) E5 (D77 v 7579696) E6 (D77 v 7579696) F1 (Standard7) F2 (Standard7) F3 (Standard8) F4 (Standard8) F5 (Standard9) F6 (Standard9) H1 (Neg Control1) H2 (Neg Control1) DetailsPlate 1 Calculations%G1 (Pos Control1) = G1/G2 = 0.85622%G2 (Pos Control1) = G1/G2 = 0.85622Group Calculations Control OID133 v 757973E)A1 = NOT INCLUDED - no result calculated)A2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D398 v 757973E)A3 = NOT INCLUDED - no result calculated)A4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. D77 v 757973E)A5 = NOT INCLUDED - no result calculated)A6 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D113 v 720281J)C1 = NOT INCLUDED - no result calculated)C2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D398 v 720281J)C3 = NOT INCLUDED - no result calculated)C4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. D77 v 720281J)C5 = NOT INCLUDED - no result calculated)C6 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D113 v 7579696)E1 = NOT INCLUDED - no result calculated)E2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D398 v 7579696)E3 = NOT INCLUDED - no result calculated)E4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. D77 v 7579696)E5 = NOT INCLUDED - no result calculated)E6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard1)B1 = NOT INCLUDED - no result calculated)B2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard2)B3 = NOT INCLUDED - no result calculated)B4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard3)B5 = NOT INCLUDED - no result calculated)B6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard4)D1 = NOT INCLUDED - no result calculated)D2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard5)D3 = NOT INCLUDED - no result calculated)D4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard6)D5 = NOT INCLUDED - no result calculated)D6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard7)F1 = NOT INCLUDED - no result calculated)F2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard8)F3 = NOT INCLUDED - no result calculated)F4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard9)F5 = NOT INCLUDED - no result calculated)F6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Pos Control1 G1 = 0.85622 G2 = 0.85622Average = 1.71244 / 2 = 0.85622 Neg Control1)H1 = NOT INCLUDED - no result calculated)H2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.#Transform (5) Validation ExpressionDetailsModule-C:\Program Files\Dazdaq\Manta\QTransforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 610,392 bytes Created = 25 June 2004, 12:01:40 CalculationsValidates: x>2.0Input MatricesControl OI () DetailsPlate 1 Calculations\Could not evaluate expression: "x>2.0" - x cannot be referenced as it has not been specified2(13 October 2004 09:59:58) Calculations, version 3Raw Data Group CalculationsPlate 10nmAnalysisTransform (1) IntegralDetailsModule-C:\Program Files\Dazdaq\Manta\KRTransform.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 680,024 bytes Created = 25 June 2004, 12:01:14 CalculationseIntegrated area by trapezium method. Using all unflagged data points, Axes = (Cycle Number, Reading).Input Matrices 0nm (RLU) Output MatricesIntegralDetailsPlate 1 Calculations)NA1 (D133 v 757973E) Integral calculated by Trapezium method; Result = 232.565NA2 (D133 v 757973E) Integral calculated by Trapezium method; Result = 215.465NA3 (D398 v 757973E) Integral calculated by Trapezium method; Result = 262.646NA4 (D398 v 757973E) Integral calculated by Trapezium method; Result = 272.436MA5 (D77 v 757973E) Integral calculated by Trapezium method; Result = 287.541MA6 (D77 v 757973E) Integral calculated by Trapezium method; Result = 207.101IB1 (Standard1) Integral calculated by Trapezium method; Result = 36.5455IB2 (Standard1) Integral calculated by Trapezium method; Result = 35.7005IB3 (Standard2) Integral calculated by Trapezium method; Result = 41.3855IB4 (Standard2) Integral calculated by Trapezium method; Result = 39.8005IB5 (Standard3) Integral calculated by Trapezium method; Result = 41.4255IB6 (Standard3) Integral calculated by Trapezium method; Result = 54.4355NC1 (D113 v 720281J) Integral calculated by Trapezium method; Result = 239.041NC2 (D113 v 720281J) Integral calculated by Trapezium method; Result = 213.441NC3 (D398 v 720281J) Integral calculated by Trapezium method; Result = 201.816NC4 (D398 v 720281J) Integral calculated by Trapezium method; Result = 188.581LC5 (D77 v 720281J) Integral calculated by Trapezium method; Result = 265.72MC6 (D77 v 720281J) Integral calculated by Trapezium method; Result = 325.116ID1 (Standard4) Integral calculated by Trapezium method; Result = 161.821ID2 (Standard4) Integral calculated by Trapezium method; Result = 57.7105ID3 (Standard5) Integral calculated by Trapezium method; Result = 68.5255ID4 (Standard5) Integral calculated by Trapezium method; Result = 64.7005ID5 (Standard6) Integral calculated by Trapezium method; Result = 69.4555ID6 (Standard6) Integral calculated by Trapezium method; Result = 64.0005ME1 (D113 v 7579696) Integral calculated by Trapezium method; Result = 277.51NE2 (D113 v 7579696) Integral calculated by Trapezium method; Result = 330.465LE3 (D398 v 7579696) Integral calculated by Trapezium method; Result = 248.5NE4 (D398 v 7579696) Integral calculated by Trapezium method; Result = 135.645ME5 (D77 v 7579696) Integral calculated by Trapezium method; Result = 160.276LE6 (D77 v 7579696) Integral calculated by Trapezium method; Result = 249.31IF1 (Standard7) Integral calculated by Trapezium method; Result = 39.1805IF2 (Standard7) Integral calculated by Trapezium method; Result = 48.6755IF3 (Standard8) Integral calculated by Trapezium method; Result = 54.8955IF4 (Standard8) Integral calculated by Trapezium method; Result = 52.7005IF5 (Standard9) Integral calculated by Trapezium method; Result = 48.5605IF6 (Standard9) Integral calculated by Trapezium method; Result = 49.7455KG1 (Pos Control1) Integral calculated by Trapezium method; Result = 189.43LG2 (Pos Control1) Integral calculated by Trapezium method; Result = 221.241LH1 (Neg Control1) Integral calculated by Trapezium method; Result = 38.3605LH2 (Neg Control1) Integral calculated by Trapezium method; Result = 44.4505Group CalculationsIntegralD133 v 757973E A1 = 232.565 A2 = 215.465Average = 448.031 / 2 = 224.015D398 v 757973E A3 = 262.646 A4 = 272.436Average = 535.081 / 2 = 267.541 D77 v 757973E A5 = 287.541 A6 = 207.101Average = 494.641 / 2 = 247.321D113 v 720281J C1 = 239.041 C2 = 213.441Average = 452.481 / 2 = 226.241D398 v 720281J C3 = 201.816 C4 = 188.581Average = 390.396 / 2 = 195.198 D77 v 720281J C5 = 265.72 C6 = 325.116Average = 590.836 / 2 = 295.418D113 v 7579696 E1 = 277.51 E2 = 330.465Average = 607.976 / 2 = 303.988D398 v 7579696 E3 = 248.5 E4 = 135.645Average = 384.146 / 2 = 192.073 D77 v 7579696 E5 = 160.276 E6 = 249.31Average = 409.586 / 2 = 204.793 Standard1 B1 = 36.5455 B2 = 35.7005Average = 72.246 / 2 = 36.123 Standard2 B3 = 41.3855 B4 = 39.8005Average = 81.186 / 2 = 40.593 Standard3 B5 = 41.4255 B6 = 54.4355Average = 95.861 / 2 = 47.9305 Standard4 D1 = 161.821 D2 = 57.7105Average = 219.531 / 2 = 109.766 Standard5 D3 = 68.5255 D4 = 64.7005Average = 133.226 / 2 = 66.613 Standard6 D5 = 69.4555 D6 = 64.0005Average = 133.456 / 2 = 66.728 Standard7 F1 = 39.1805 F2 = 48.6755Average = 87.856 / 2 = 43.928 Standard8 F3 = 54.8955 F4 = 52.7005Average = 107.596 / 2 = 53.798 Standard9 F5 = 48.5605 F6 = 49.7455Average = 98.306 / 2 = 49.153 Pos Control1 G1 = 189.43 G2 = 221.241Average = 410.671 / 2 = 205.335 Neg Control1 H1 = 38.3605 H2 = 44.4505Average = 82.811 / 2 = 41.4055Transform (2) Matrix ExpressionDetailsModule,C:\Program Files\Dazdaq\Manta\Transforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 888,918 bytes Created = 25 June 2004, 12:01:04 Calculations "w/w(0,1)"Input Matrices Integral () Output Matrices Opsonic IndexNot CalculatedB1 (Standard1) B2 (Standard1) B3 (Standard2) B4 (Standard2) B5 (Standard3) B6 (Standard3) D1 (Standard4) D2 (Standard4) D3 (Standard5) D4 (Standard5) D5 (Standard6) D6 (Standard6) F1 (Standard7) F2 (Standard7) F3 (Standard8) F4 (Standard8) F5 (Standard9) F6 (Standard9) DetailsPlate 1 Calculations*A1 (D133 v 757973E) = w/w(0,1) = 6.36372*A2 (D133 v 757973E) = w/w(0,1) = 6.03536*A3 (D398 v 757973E) = w/w(0,1) = 6.34632*A4 (D398 v 757973E) = w/w(0,1) = 6.84503)A5 (D77 v 757973E) = w/w(0,1) = 6.94115)A6 (D77 v 757973E) = w/w(0,1) = 3.80451)C1 (D113 v 720281J) = w/w(0,1) = 1.4772*C2 (D113 v 720281J) = w/w(0,1) = 3.69847*C3 (D398 v 720281J) = w/w(0,1) = 2.94512*C4 (D398 v 720281J) = w/w(0,1) = 2.91467)C5 (D77 v 720281J) = w/w(0,1) = 3.82577)C6 (D77 v 720281J) = w/w(0,1) = 5.07989*E1 (D113 v 7579696) = w/w(0,1) = 7.08287*E2 (D113 v 7579696) = w/w(0,1) = 6.78915*E3 (D398 v 7579696) = w/w(0,1) = 4.52679*E4 (D398 v 7579696) = w/w(0,1) = 2.57389)E5 (D77 v 7579696) = w/w(0,1) = 3.30053)E6 (D77 v 7579696) = w/w(0,1) = 5.01172(G1 (Pos Control1) = w/w(0,1) = 4.93817(G2 (Pos Control1) = w/w(0,1) = 4.97723WH1 (Neg Control1) Could not evaluate: "w/w(0,1)" - Well offset out of microplate rangeWH2 (Neg Control1) Could not evaluate: "w/w(0,1)" - Well offset out of microplate rangeGroup Calculations Opsonic IndexD133 v 757973E A1 = 6.36372 A2 = 6.03536Average = 12.3991 / 2 = 6.19954D398 v 757973E A3 = 6.34632 A4 = 6.84503Average = 13.1913 / 2 = 6.59567 D77 v 757973E A5 = 6.94115 A6 = 3.80451Average = 10.7457 / 2 = 5.37283D113 v 720281J C1 = 1.4772 C2 = 3.69847Average = 5.17566 / 2 = 2.58783D398 v 720281J C3 = 2.94512 C4 = 2.91467Average = 5.85978 / 2 = 2.92989 D77 v 720281J C5 = 3.82577 C6 = 5.07989Average = 8.90566 / 2 = 4.45283D113 v 7579696 E1 = 7.08287 E2 = 6.78915Average = 13.872 / 2 = 6.93601D398 v 7579696 E3 = 4.52679 E4 = 2.57389Average = 7.10069 / 2 = 3.55034 D77 v 7579696 E5 = 3.30053 E6 = 5.01172Average = 8.31225 / 2 = 4.15613 Standard1)B1 = NOT INCLUDED - no result calculated)B2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard2)B3 = NOT INCLUDED - no result calculated)B4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard3)B5 = NOT INCLUDED - no result calculated)B6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard4)D1 = NOT INCLUDED - no result calculated)D2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard5)D3 = NOT INCLUDED - no result calculated)D4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard6)D5 = NOT INCLUDED - no result calculated)D6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard7)F1 = NOT INCLUDED - no result calculated)F2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard8)F3 = NOT INCLUDED - no result calculated)F4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard9)F5 = NOT INCLUDED - no result calculated)F6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Pos Control1 G1 = 4.93817 G2 = 4.97723Average = 9.9154 / 2 = 4.9577 Neg Control1&H1 = NOT INCLUDED - calculation error&H2 = NOT INCLUDED - calculation error&All wells in this group were excluded.Transform (3) Auto Flag By WellDetailsModule,C:\Program Files\Dazdaq\Manta\Transforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 888,918 bytes Created = 25 June 2004, 12:01:04 Calculationsx>1.2Input MatricesOpsonic Index () Not CalculatedB1 (Standard1) B2 (Standard1) B3 (Standard2) B4 (Standard2) B5 (Standard3) B6 (Standard3) D1 (Standard4) D2 (Standard4) D3 (Standard5) D4 (Standard5) D5 (Standard6) D6 (Standard6) F1 (Standard7) F2 (Standard7) F3 (Standard8) F4 (Standard8) F5 (Standard9) F6 (Standard9) G1 (Pos Control1) G2 (Pos Control1) H1 (Neg Control1) H2 (Neg Control1) DetailsPlate 1 CalculationsExpression = x>1.2BA1 (D133 v 757973E) x = 6.36372, Result = TRUE, Well AUTO-FLAGGEDBA2 (D133 v 757973E) x = 6.03536, Result = TRUE, Well AUTO-FLAGGEDBA3 (D398 v 757973E) x = 6.34632, Result = TRUE, Well AUTO-FLAGGEDBA4 (D398 v 757973E) x = 6.84503, Result = TRUE, Well AUTO-FLAGGEDAA5 (D77 v 757973E) x = 6.94115, Result = TRUE, Well AUTO-FLAGGEDAA6 (D77 v 757973E) x = 3.80451, Result = TRUE, Well AUTO-FLAGGEDAC1 (D113 v 720281J) x = 1.4772, Result = TRUE, Well AUTO-FLAGGEDBC2 (D113 v 720281J) x = 3.69847, Result = TRUE, Well AUTO-FLAGGEDBC3 (D398 v 720281J) x = 2.94512, Result = TRUE, Well AUTO-FLAGGEDBC4 (D398 v 720281J) x = 2.91467, Result = TRUE, Well AUTO-FLAGGEDAC5 (D77 v 720281J) x = 3.82577, Result = TRUE, Well AUTO-FLAGGEDAC6 (D77 v 720281J) x = 5.07989, Result = TRUE, Well AUTO-FLAGGEDBE1 (D113 v 7579696) x = 7.08287, Result = TRUE, Well AUTO-FLAGGEDBE2 (D113 v 7579696) x = 6.78915, Result = TRUE, Well AUTO-FLAGGEDBE3 (D398 v 7579696) x = 4.52679, Result = TRUE, Well AUTO-FLAGGEDBE4 (D398 v 7579696) x = 2.57389, Result = TRUE, Well AUTO-FLAGGEDAE5 (D77 v 7579696) x = 3.30053, Result = TRUE, Well AUTO-FLAGGEDAE6 (D77 v 7579696) x = 5.01172, Result = TRUE, Well AUTO-FLAGGEDTransform (4) Matrix ExpressionDetailsModule,C:\Program Files\Dazdaq\Manta\Transforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 888,918 bytes Created = 25 June 2004, 12:01:04 Calculations "w/w(0,1)"Input Matrices Integral () Output Matrices Control OINot Calculated&A1 (D133 v 757973E) A2 (D133 v 757973E) A3 (D398 v 757973E) A4 (D398 v 757973E) A5 (D77 v 757973E) A6 (D77 v 757973E) B1 (Standard1) B2 (Standard1) B3 (Standard2) B4 (Standard2) B5 (Standard3) B6 (Standard3) C1 (D113 v 720281J) C2 (D113 v 720281J) C3 (D398 v 720281J) C4 (D398 v 720281J) C5 (D77 v 720281J) C6 (D77 v 720281J) D1 (Standard4) D2 (Standard4) D3 (Standard5) D4 (Standard5) D5 (Standard6) D6 (Standard6) E1 (D113 v 7579696) E2 (D113 v 7579696) E3 (D398 v 7579696) E4 (D398 v 7579696) E5 (D77 v 7579696) E6 (D77 v 7579696) F1 (Standard7) F2 (Standard7) F3 (Standard8) F4 (Standard8) F5 (Standard9) F6 (Standard9) H1 (Neg Control1) H2 (Neg Control1) DetailsPlate 1 Calculations(G1 (Pos Control1) = w/w(0,1) = 4.93817(G2 (Pos Control1) = w/w(0,1) = 4.97723Group Calculations Control OID133 v 757973E)A1 = NOT INCLUDED - no result calculated)A2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D398 v 757973E)A3 = NOT INCLUDED - no result calculated)A4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. D77 v 757973E)A5 = NOT INCLUDED - no result calculated)A6 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D113 v 720281J)C1 = NOT INCLUDED - no result calculated)C2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D398 v 720281J)C3 = NOT INCLUDED - no result calculated)C4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. D77 v 720281J)C5 = NOT INCLUDED - no result calculated)C6 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D113 v 7579696)E1 = NOT INCLUDED - no result calculated)E2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D398 v 7579696)E3 = NOT INCLUDED - no result calculated)E4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. D77 v 7579696)E5 = NOT INCLUDED - no result calculated)E6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard1)B1 = NOT INCLUDED - no result calculated)B2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard2)B3 = NOT INCLUDED - no result calculated)B4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard3)B5 = NOT INCLUDED - no result calculated)B6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard4)D1 = NOT INCLUDED - no result calculated)D2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard5)D3 = NOT INCLUDED - no result calculated)D4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard6)D5 = NOT INCLUDED - no result calculated)D6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard7)F1 = NOT INCLUDED - no result calculated)F2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard8)F3 = NOT INCLUDED - no result calculated)F4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard9)F5 = NOT INCLUDED - no result calculated)F6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Pos Control1 G1 = 4.93817 G2 = 4.97723Average = 9.9154 / 2 = 4.9577 Neg Control1)H1 = NOT INCLUDED - no result calculated)H2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.#Transform (5) Validation ExpressionDetailsModule-C:\Program Files\Dazdaq\Manta\QTransforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 610,392 bytes Created = 25 June 2004, 12:01:40 CalculationsValidates: x>2.0Input MatricesControl OI () DetailsPlate 1 Calculations\Could not evaluate expression: "x>2.0" - x cannot be referenced as it has not been specified2(13 October 2004 10:01:07) Calculations, version 4Raw Data Group CalculationsPlate 10nmAnalysisTransform (1) IntegralDetailsModule-C:\Program Files\Dazdaq\Manta\KRTransform.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 680,024 bytes Created = 25 June 2004, 12:01:14 CalculationseIntegrated area by trapezium method. Using all unflagged data points, Axes = (Cycle Number, Reading).Input Matrices 0nm (RLU) Output MatricesIntegralDetailsPlate 1 Calculations)NA1 (D133 v 757973E) Integral calculated by Trapezium method; Result = 232.565NA2 (D133 v 757973E) Integral calculated by Trapezium method; Result = 215.465NA3 (D398 v 757973E) Integral calculated by Trapezium method; Result = 262.646NA4 (D398 v 757973E) Integral calculated by Trapezium method; Result = 272.436MA5 (D77 v 757973E) Integral calculated by Trapezium method; Result = 287.541MA6 (D77 v 757973E) Integral calculated by Trapezium method; Result = 207.101IB1 (Standard1) Integral calculated by Trapezium method; Result = 36.5455IB2 (Standard1) Integral calculated by Trapezium method; Result = 35.7005IB3 (Standard2) Integral calculated by Trapezium method; Result = 41.3855IB4 (Standard2) Integral calculated by Trapezium method; Result = 39.8005IB5 (Standard3) Integral calculated by Trapezium method; Result = 41.4255IB6 (Standard3) Integral calculated by Trapezium method; Result = 54.4355NC1 (D113 v 720281J) Integral calculated by Trapezium method; Result = 239.041NC2 (D113 v 720281J) Integral calculated by Trapezium method; Result = 213.441NC3 (D398 v 720281J) Integral calculated by Trapezium method; Result = 201.816NC4 (D398 v 720281J) Integral calculated by Trapezium method; Result = 188.581LC5 (D77 v 720281J) Integral calculated by Trapezium method; Result = 265.72MC6 (D77 v 720281J) Integral calculated by Trapezium method; Result = 325.116ID1 (Standard4) Integral calculated by Trapezium method; Result = 161.821ID2 (Standard4) Integral calculated by Trapezium method; Result = 57.7105ID3 (Standard5) Integral calculated by Trapezium method; Result = 68.5255ID4 (Standard5) Integral calculated by Trapezium method; Result = 64.7005ID5 (Standard6) Integral calculated by Trapezium method; Result = 69.4555ID6 (Standard6) Integral calculated by Trapezium method; Result = 64.0005ME1 (D113 v 7579696) Integral calculated by Trapezium method; Result = 277.51NE2 (D113 v 7579696) Integral calculated by Trapezium method; Result = 330.465LE3 (D398 v 7579696) Integral calculated by Trapezium method; Result = 248.5NE4 (D398 v 7579696) Integral calculated by Trapezium method; Result = 135.645ME5 (D77 v 7579696) Integral calculated by Trapezium method; Result = 160.276LE6 (D77 v 7579696) Integral calculated by Trapezium method; Result = 249.31IF1 (Standard7) Integral calculated by Trapezium method; Result = 39.1805IF2 (Standard7) Integral calculated by Trapezium method; Result = 48.6755IF3 (Standard8) Integral calculated by Trapezium method; Result = 54.8955IF4 (Standard8) Integral calculated by Trapezium method; Result = 52.7005IF5 (Standard9) Integral calculated by Trapezium method; Result = 48.5605IF6 (Standard9) Integral calculated by Trapezium method; Result = 49.7455KG1 (Pos Control1) Integral calculated by Trapezium method; Result = 189.43LG2 (Pos Control1) Integral calculated by Trapezium method; Result = 221.241LH1 (Neg Control1) Integral calculated by Trapezium method; Result = 38.3605LH2 (Neg Control1) Integral calculated by Trapezium method; Result = 44.4505Group CalculationsIntegralD133 v 757973E A1 = 232.565 A2 = 215.465Average = 448.031 / 2 = 224.015D398 v 757973E A3 = 262.646 A4 = 272.436Average = 535.081 / 2 = 267.541 D77 v 757973E A5 = 287.541 A6 = 207.101Average = 494.641 / 2 = 247.321D113 v 720281J C1 = 239.041 C2 = 213.441Average = 452.481 / 2 = 226.241D398 v 720281J C3 = 201.816 C4 = 188.581Average = 390.396 / 2 = 195.198 D77 v 720281J C5 = 265.72 C6 = 325.116Average = 590.836 / 2 = 295.418D113 v 7579696 E1 = 277.51 E2 = 330.465Average = 607.976 / 2 = 303.988D398 v 7579696 E3 = 248.5 E4 = 135.645Average = 384.146 / 2 = 192.073 D77 v 7579696 E5 = 160.276 E6 = 249.31Average = 409.586 / 2 = 204.793 Standard1 B1 = 36.5455 B2 = 35.7005Average = 72.246 / 2 = 36.123 Standard2 B3 = 41.3855 B4 = 39.8005Average = 81.186 / 2 = 40.593 Standard3 B5 = 41.4255 B6 = 54.4355Average = 95.861 / 2 = 47.9305 Standard4 D1 = 161.821 D2 = 57.7105Average = 219.531 / 2 = 109.766 Standard5 D3 = 68.5255 D4 = 64.7005Average = 133.226 / 2 = 66.613 Standard6 D5 = 69.4555 D6 = 64.0005Average = 133.456 / 2 = 66.728 Standard7 F1 = 39.1805 F2 = 48.6755Average = 87.856 / 2 = 43.928 Standard8 F3 = 54.8955 F4 = 52.7005Average = 107.596 / 2 = 53.798 Standard9 F5 = 48.5605 F6 = 49.7455Average = 98.306 / 2 = 49.153 Pos Control1 G1 = 189.43 G2 = 221.241Average = 410.671 / 2 = 205.335 Neg Control1 H1 = 38.3605 H2 = 44.4505Average = 82.811 / 2 = 41.4055Transform (2) Matrix ExpressionDetailsModule,C:\Program Files\Dazdaq\Manta\Transforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 888,918 bytes Created = 25 June 2004, 12:01:04 Calculations "w/w(0,1)"Input Matrices Integral () Output Matrices Opsonic IndexNot CalculatedB1 (Standard1) B2 (Standard1) B3 (Standard2) B4 (Standard2) B5 (Standard3) B6 (Standard3) D1 (Standard4) D2 (Standard4) D3 (Standard5) D4 (Standard5) D5 (Standard6) D6 (Standard6) F1 (Standard7) F2 (Standard7) F3 (Standard8) F4 (Standard8) F5 (Standard9) F6 (Standard9) DetailsPlate 1 Calculations*A1 (D133 v 757973E) = w/w(0,1) = 6.36372*A2 (D133 v 757973E) = w/w(0,1) = 6.03536*A3 (D398 v 757973E) = w/w(0,1) = 6.34632*A4 (D398 v 757973E) = w/w(0,1) = 6.84503)A5 (D77 v 757973E) = w/w(0,1) = 6.94115)A6 (D77 v 757973E) = w/w(0,1) = 3.80451)C1 (D113 v 720281J) = w/w(0,1) = 1.4772*C2 (D113 v 720281J) = w/w(0,1) = 3.69847*C3 (D398 v 720281J) = w/w(0,1) = 2.94512*C4 (D398 v 720281J) = w/w(0,1) = 2.91467)C5 (D77 v 720281J) = w/w(0,1) = 3.82577)C6 (D77 v 720281J) = w/w(0,1) = 5.07989*E1 (D113 v 7579696) = w/w(0,1) = 7.08287*E2 (D113 v 7579696) = w/w(0,1) = 6.78915*E3 (D398 v 7579696) = w/w(0,1) = 4.52679*E4 (D398 v 7579696) = w/w(0,1) = 2.57389)E5 (D77 v 7579696) = w/w(0,1) = 3.30053)E6 (D77 v 7579696) = w/w(0,1) = 5.01172(G1 (Pos Control1) = w/w(0,1) = 4.93817(G2 (Pos Control1) = w/w(0,1) = 4.97723WH1 (Neg Control1) Could not evaluate: "w/w(0,1)" - Well offset out of microplate rangeWH2 (Neg Control1) Could not evaluate: "w/w(0,1)" - Well offset out of microplate rangeGroup Calculations Opsonic IndexD133 v 757973E A1 = 6.36372 A2 = 6.03536Average = 12.3991 / 2 = 6.19954D398 v 757973E A3 = 6.34632 A4 = 6.84503Average = 13.1913 / 2 = 6.59567 D77 v 757973E A5 = 6.94115 A6 = 3.80451Average = 10.7457 / 2 = 5.37283D113 v 720281J C1 = 1.4772 C2 = 3.69847Average = 5.17566 / 2 = 2.58783D398 v 720281J C3 = 2.94512 C4 = 2.91467Average = 5.85978 / 2 = 2.92989 D77 v 720281J C5 = 3.82577 C6 = 5.07989Average = 8.90566 / 2 = 4.45283D113 v 7579696 E1 = 7.08287 E2 = 6.78915Average = 13.872 / 2 = 6.93601D398 v 7579696 E3 = 4.52679 E4 = 2.57389Average = 7.10069 / 2 = 3.55034 D77 v 7579696 E5 = 3.30053 E6 = 5.01172Average = 8.31225 / 2 = 4.15613 Standard1)B1 = NOT INCLUDED - no result calculated)B2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard2)B3 = NOT INCLUDED - no result calculated)B4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard3)B5 = NOT INCLUDED - no result calculated)B6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard4)D1 = NOT INCLUDED - no result calculated)D2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard5)D3 = NOT INCLUDED - no result calculated)D4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard6)D5 = NOT INCLUDED - no result calculated)D6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard7)F1 = NOT INCLUDED - no result calculated)F2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard8)F3 = NOT INCLUDED - no result calculated)F4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard9)F5 = NOT INCLUDED - no result calculated)F6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Pos Control1 G1 = 4.93817 G2 = 4.97723Average = 9.9154 / 2 = 4.9577 Neg Control1&H1 = NOT INCLUDED - calculation error&H2 = NOT INCLUDED - calculation error&All wells in this group were excluded.Transform (3) Auto Flag By WellDetailsModule,C:\Program Files\Dazdaq\Manta\Transforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 888,918 bytes Created = 25 June 2004, 12:01:04 Calculationsx>1.2Input MatricesOpsonic Index () Not CalculatedB1 (Standard1) B2 (Standard1) B3 (Standard2) B4 (Standard2) B5 (Standard3) B6 (Standard3) D1 (Standard4) D2 (Standard4) D3 (Standard5) D4 (Standard5) D5 (Standard6) D6 (Standard6) F1 (Standard7) F2 (Standard7) F3 (Standard8) F4 (Standard8) F5 (Standard9) F6 (Standard9) G1 (Pos Control1) G2 (Pos Control1) H1 (Neg Control1) H2 (Neg Control1) DetailsPlate 1 CalculationsExpression = x>1.2BA1 (D133 v 757973E) x = 6.36372, Result = TRUE, Well AUTO-FLAGGEDBA2 (D133 v 757973E) x = 6.03536, Result = TRUE, Well AUTO-FLAGGEDBA3 (D398 v 757973E) x = 6.34632, Result = TRUE, Well AUTO-FLAGGEDBA4 (D398 v 757973E) x = 6.84503, Result = TRUE, Well AUTO-FLAGGEDAA5 (D77 v 757973E) x = 6.94115, Result = TRUE, Well AUTO-FLAGGEDAA6 (D77 v 757973E) x = 3.80451, Result = TRUE, Well AUTO-FLAGGEDAC1 (D113 v 720281J) x = 1.4772, Result = TRUE, Well AUTO-FLAGGEDBC2 (D113 v 720281J) x = 3.69847, Result = TRUE, Well AUTO-FLAGGEDBC3 (D398 v 720281J) x = 2.94512, Result = TRUE, Well AUTO-FLAGGEDBC4 (D398 v 720281J) x = 2.91467, Result = TRUE, Well AUTO-FLAGGEDAC5 (D77 v 720281J) x = 3.82577, Result = TRUE, Well AUTO-FLAGGEDAC6 (D77 v 720281J) x = 5.07989, Result = TRUE, Well AUTO-FLAGGEDBE1 (D113 v 7579696) x = 7.08287, Result = TRUE, Well AUTO-FLAGGEDBE2 (D113 v 7579696) x = 6.78915, Result = TRUE, Well AUTO-FLAGGEDBE3 (D398 v 7579696) x = 4.52679, Result = TRUE, Well AUTO-FLAGGEDBE4 (D398 v 7579696) x = 2.57389, Result = TRUE, Well AUTO-FLAGGEDAE5 (D77 v 7579696) x = 3.30053, Result = TRUE, Well AUTO-FLAGGEDAE6 (D77 v 7579696) x = 5.01172, Result = TRUE, Well AUTO-FLAGGEDTransform (4) Matrix ExpressionDetailsModule,C:\Program Files\Dazdaq\Manta\Transforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 888,918 bytes Created = 25 June 2004, 12:01:04 Calculations "w/w(0,1)"Input Matrices Integral () Output Matrices Control OINot Calculated&A1 (D133 v 757973E) A2 (D133 v 757973E) A3 (D398 v 757973E) A4 (D398 v 757973E) A5 (D77 v 757973E) A6 (D77 v 757973E) B1 (Standard1) B2 (Standard1) B3 (Standard2) B4 (Standard2) B5 (Standard3) B6 (Standard3) C1 (D113 v 720281J) C2 (D113 v 720281J) C3 (D398 v 720281J) C4 (D398 v 720281J) C5 (D77 v 720281J) C6 (D77 v 720281J) D1 (Standard4) D2 (Standard4) D3 (Standard5) D4 (Standard5) D5 (Standard6) D6 (Standard6) E1 (D113 v 7579696) E2 (D113 v 7579696) E3 (D398 v 7579696) E4 (D398 v 7579696) E5 (D77 v 7579696) E6 (D77 v 7579696) F1 (Standard7) F2 (Standard7) F3 (Standard8) F4 (Standard8) F5 (Standard9) F6 (Standard9) H1 (Neg Control1) H2 (Neg Control1) DetailsPlate 1 Calculations(G1 (Pos Control1) = w/w(0,1) = 4.93817(G2 (Pos Control1) = w/w(0,1) = 4.97723Group Calculations Control OID133 v 757973E)A1 = NOT INCLUDED - no result calculated)A2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D398 v 757973E)A3 = NOT INCLUDED - no result calculated)A4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. D77 v 757973E)A5 = NOT INCLUDED - no result calculated)A6 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D113 v 720281J)C1 = NOT INCLUDED - no result calculated)C2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D398 v 720281J)C3 = NOT INCLUDED - no result calculated)C4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. D77 v 720281J)C5 = NOT INCLUDED - no result calculated)C6 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D113 v 7579696)E1 = NOT INCLUDED - no result calculated)E2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D398 v 7579696)E3 = NOT INCLUDED - no result calculated)E4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. D77 v 7579696)E5 = NOT INCLUDED - no result calculated)E6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard1)B1 = NOT INCLUDED - no result calculated)B2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard2)B3 = NOT INCLUDED - no result calculated)B4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard3)B5 = NOT INCLUDED - no result calculated)B6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard4)D1 = NOT INCLUDED - no result calculated)D2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard5)D3 = NOT INCLUDED - no result calculated)D4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard6)D5 = NOT INCLUDED - no result calculated)D6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard7)F1 = NOT INCLUDED - no result calculated)F2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard8)F3 = NOT INCLUDED - no result calculated)F4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard9)F5 = NOT INCLUDED - no result calculated)F6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Pos Control1 G1 = 4.93817 G2 = 4.97723Average = 9.9154 / 2 = 4.9577 Neg Control1)H1 = NOT INCLUDED - no result calculated)H2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.#Transform (5) Validation ExpressionDetailsModule-C:\Program Files\Dazdaq\Manta\QTransforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 610,392 bytes Created = 25 June 2004, 12:01:40 CalculationsValidates: x>2.0Input MatricesControl OI () DetailsPlate 1 Calculations\Could not evaluate expression: "x>2.0" - x cannot be referenced as it has not been specified2(13 October 2004 10:02:05) Calculations, version 5Raw Data Group CalculationsPlate 10nmAnalysisTransform (1) IntegralDetailsModule-C:\Program Files\Dazdaq\Manta\KRTransform.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 680,024 bytes Created = 25 June 2004, 12:01:14 CalculationseIntegrated area by trapezium method. Using all unflagged data points, Axes = (Cycle Number, Reading).Input Matrices 0nm (RLU) Output MatricesIntegralDetailsPlate 1 Calculations)NA1 (D133 v 757973E) Integral calculated by Trapezium method; Result = 232.565NA2 (D133 v 757973E) Integral calculated by Trapezium method; Result = 215.465NA3 (D398 v 757973E) Integral calculated by Trapezium method; Result = 262.646NA4 (D398 v 757973E) Integral calculated by Trapezium method; Result = 272.435MA5 (D77 v 757973E) Integral calculated by Trapezium method; Result = 287.541MA6 (D77 v 757973E) Integral calculated by Trapezium method; Result = 207.101IB1 (Standard1) Integral calculated by Trapezium method; Result = 36.5455IB2 (Standard1) Integral calculated by Trapezium method; Result = 35.7005IB3 (Standard2) Integral calculated by Trapezium method; Result = 41.3855IB4 (Standard2) Integral calculated by Trapezium method; Result = 39.8005IB5 (Standard3) Integral calculated by Trapezium method; Result = 41.4255IB6 (Standard3) Integral calculated by Trapezium method; Result = 54.4355NC1 (D113 v 720281J) Integral calculated by Trapezium method; Result = 239.041MC2 (D113 v 720281J) Integral calculated by Trapezium method; Result = 213.44NC3 (D398 v 720281J) Integral calculated by Trapezium method; Result = 201.816NC4 (D398 v 720281J) Integral calculated by Trapezium method; Result = 188.581MC5 (D77 v 720281J) Integral calculated by Trapezium method; Result = 265.721MC6 (D77 v 720281J) Integral calculated by Trapezium method; Result = 325.116ID1 (Standard4) Integral calculated by Trapezium method; Result = 161.821ID2 (Standard4) Integral calculated by Trapezium method; Result = 57.7105ID3 (Standard5) Integral calculated by Trapezium method; Result = 68.5255ID4 (Standard5) Integral calculated by Trapezium method; Result = 64.7005ID5 (Standard6) Integral calculated by Trapezium method; Result = 69.4555ID6 (Standard6) Integral calculated by Trapezium method; Result = 64.0005ME1 (D113 v 7579696) Integral calculated by Trapezium method; Result = 277.51NE2 (D113 v 7579696) Integral calculated by Trapezium method; Result = 330.466LE3 (D398 v 7579696) Integral calculated by Trapezium method; Result = 248.5NE4 (D398 v 7579696) Integral calculated by Trapezium method; Result = 135.646ME5 (D77 v 7579696) Integral calculated by Trapezium method; Result = 160.275LE6 (D77 v 7579696) Integral calculated by Trapezium method; Result = 249.31IF1 (Standard7) Integral calculated by Trapezium method; Result = 39.1805IF2 (Standard7) Integral calculated by Trapezium method; Result = 48.6755IF3 (Standard8) Integral calculated by Trapezium method; Result = 54.8955IF4 (Standard8) Integral calculated by Trapezium method; Result = 52.7005IF5 (Standard9) Integral calculated by Trapezium method; Result = 48.5605IF6 (Standard9) Integral calculated by Trapezium method; Result = 49.7455KG1 (Pos Control1) Integral calculated by Trapezium method; Result = 189.43LG2 (Pos Control1) Integral calculated by Trapezium method; Result = 221.241LH1 (Neg Control1) Integral calculated by Trapezium method; Result = 38.3605LH2 (Neg Control1) Integral calculated by Trapezium method; Result = 44.4505Group CalculationsIntegralD133 v 757973E A1 = 232.565 A2 = 215.465Average = 448.031 / 2 = 224.015D398 v 757973E A3 = 262.646 A4 = 272.435Average = 535.081 / 2 = 267.541 D77 v 757973E A5 = 287.541 A6 = 207.101Average = 494.641 / 2 = 247.321D113 v 720281J C1 = 239.041 C2 = 213.44Average = 452.481 / 2 = 226.241D398 v 720281J C3 = 201.816 C4 = 188.581Average = 390.396 / 2 = 195.198 D77 v 720281J C5 = 265.721 C6 = 325.116Average = 590.836 / 2 = 295.418D113 v 7579696 E1 = 277.51 E2 = 330.466Average = 607.976 / 2 = 303.988D398 v 7579696 E3 = 248.5 E4 = 135.646Average = 384.146 / 2 = 192.073 D77 v 7579696 E5 = 160.275 E6 = 249.31Average = 409.586 / 2 = 204.793 Standard1 B1 = 36.5455 B2 = 35.7005Average = 72.246 / 2 = 36.123 Standard2 B3 = 41.3855 B4 = 39.8005Average = 81.186 / 2 = 40.593 Standard3 B5 = 41.4255 B6 = 54.4355Average = 95.861 / 2 = 47.9305 Standard4 D1 = 161.821 D2 = 57.7105Average = 219.531 / 2 = 109.766 Standard5 D3 = 68.5255 D4 = 64.7005Average = 133.226 / 2 = 66.613 Standard6 D5 = 69.4555 D6 = 64.0005Average = 133.456 / 2 = 66.728 Standard7 F1 = 39.1805 F2 = 48.6755Average = 87.856 / 2 = 43.928 Standard8 F3 = 54.8955 F4 = 52.7005Average = 107.      !"#$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcdefghijklmnopqrstuvwxyz{|}~596 / 2 = 53.798 Standard9 F5 = 48.5605 F6 = 49.7455Average = 98.306 / 2 = 49.153 Pos Control1 G1 = 189.43 G2 = 221.241Average = 410.671 / 2 = 205.336 Neg Control1 H1 = 38.3605 H2 = 44.4505Average = 82.811 / 2 = 41.4055Transform (2) Matrix ExpressionDetailsModule,C:\Program Files\Dazdaq\Manta\Transforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 888,918 bytes Created = 25 June 2004, 12:01:04 Calculations "w/w(0,1)"Input Matrices Integral () Output Matrices Opsonic IndexNot CalculatedB1 (Standard1) B2 (Standard1) B3 (Standard2) B4 (Standard2) B5 (Standard3) B6 (Standard3) D1 (Standard4) D2 (Standard4) D3 (Standard5) D4 (Standard5) D5 (Standard6) D6 (Standard6) F1 (Standard7) F2 (Standard7) F3 (Standard8) F4 (Standard8) F5 (Standard9) F6 (Standard9) DetailsPlate 1 Calculations*A1 (D133 v 757973E) = w/w(0,1) = 6.36372*A2 (D133 v 757973E) = w/w(0,1) = 6.03536*A3 (D398 v 757973E) = w/w(0,1) = 6.34632*A4 (D398 v 757973E) = w/w(0,1) = 6.84503)A5 (D77 v 757973E) = w/w(0,1) = 6.94115)A6 (D77 v 757973E) = w/w(0,1) = 3.80451)C1 (D113 v 720281J) = w/w(0,1) = 1.4772*C2 (D113 v 720281J) = w/w(0,1) = 3.69847*C3 (D398 v 720281J) = w/w(0,1) = 2.94512*C4 (D398 v 720281J) = w/w(0,1) = 2.91467)C5 (D77 v 720281J) = w/w(0,1) = 3.82577)C6 (D77 v 720281J) = w/w(0,1) = 5.07989*E1 (D113 v 7579696) = w/w(0,1) = 7.08287*E2 (D113 v 7579696) = w/w(0,1) = 6.78915*E3 (D398 v 7579696) = w/w(0,1) = 4.52679*E4 (D398 v 7579696) = w/w(0,1) = 2.57389)E5 (D77 v 7579696) = w/w(0,1) = 3.30053)E6 (D77 v 7579696) = w/w(0,1) = 5.01172(G1 (Pos Control1) = w/w(0,1) = 4.93817(G2 (Pos Control1) = w/w(0,1) = 4.97723WH1 (Neg Control1) Could not evaluate: "w/w(0,1)" - Well offset out of microplate rangeWH2 (Neg Control1) Could not evaluate: "w/w(0,1)" - Well offset out of microplate rangeGroup Calculations Opsonic IndexD133 v 757973E A1 = 6.36372 A2 = 6.03536Average = 12.3991 / 2 = 6.19954D398 v 757973E A3 = 6.34632 A4 = 6.84503Average = 13.1913 / 2 = 6.59567 D77 v 757973E A5 = 6.94115 A6 = 3.80451Average = 10.7457 / 2 = 5.37283D113 v 720281J C1 = 1.4772 C2 = 3.69847Average = 5.17566 / 2 = 2.58783D398 v 720281J C3 = 2.94512 C4 = 2.91467Average = 5.85978 / 2 = 2.92989 D77 v 720281J C5 = 3.82577 C6 = 5.07989Average = 8.90566 / 2 = 4.45283D113 v 7579696 E1 = 7.08287 E2 = 6.78915Average = 13.872 / 2 = 6.93601D398 v 7579696 E3 = 4.52679 E4 = 2.57389Average = 7.10069 / 2 = 3.55034 D77 v 7579696 E5 = 3.30053 E6 = 5.01172Average = 8.31225 / 2 = 4.15613 Standard1)B1 = NOT INCLUDED - no result calculated)B2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard2)B3 = NOT INCLUDED - no result calculated)B4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard3)B5 = NOT INCLUDED - no result calculated)B6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard4)D1 = NOT INCLUDED - no result calculated)D2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard5)D3 = NOT INCLUDED - no result calculated)D4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard6)D5 = NOT INCLUDED - no result calculated)D6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard7)F1 = NOT INCLUDED - no result calculated)F2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard8)F3 = NOT INCLUDED - no result calculated)F4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard9)F5 = NOT INCLUDED - no result calculated)F6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Pos Control1 G1 = 4.93817 G2 = 4.97723Average = 9.9154 / 2 = 4.9577 Neg Control1&H1 = NOT INCLUDED - calculation error&H2 = NOT INCLUDED - calculation error&All wells in this group were excluded.Transform (3) Auto Flag By WellDetailsModule,C:\Program Files\Dazdaq\Manta\Transforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 888,918 bytes Created = 25 June 2004, 12:01:04 Calculationsx>1.2Input MatricesOpsonic Index () Not CalculatedB1 (Standard1) B2 (Standard1) B3 (Standard2) B4 (Standard2) B5 (Standard3) B6 (Standard3) D1 (Standard4) D2 (Standard4) D3 (Standard5) D4 (Standard5) D5 (Standard6) D6 (Standard6) F1 (Standard7) F2 (Standard7) F3 (Standard8) F4 (Standard8) F5 (Standard9) F6 (Standard9) G1 (Pos Control1) G2 (Pos Control1) H1 (Neg Control1) H2 (Neg Control1) DetailsPlate 1 CalculationsExpression = x>1.2BA1 (D133 v 757973E) x = 6.36372, Result = TRUE, Well AUTO-FLAGGEDBA2 (D133 v 757973E) x = 6.03536, Result = TRUE, Well AUTO-FLAGGEDBA3 (D398 v 757973E) x = 6.34632, Result = TRUE, Well AUTO-FLAGGEDBA4 (D398 v 757973E) x = 6.84503, Result = TRUE, Well AUTO-FLAGGEDAA5 (D77 v 757973E) x = 6.94115, Result = TRUE, Well AUTO-FLAGGEDAA6 (D77 v 757973E) x = 3.80451, Result = TRUE, Well AUTO-FLAGGEDAC1 (D113 v 720281J) x = 1.4772, Result = TRUE, Well AUTO-FLAGGEDBC2 (D113 v 720281J) x = 3.69847, Result = TRUE, Well AUTO-FLAGGEDBC3 (D398 v 720281J) x = 2.94512, Result = TRUE, Well AUTO-FLAGGEDBC4 (D398 v 720281J) x = 2.91467, Result = TRUE, Well AUTO-FLAGGEDAC5 (D77 v 720281J) x = 3.82577, Result = TRUE, Well AUTO-FLAGGEDAC6 (D77 v 720281J) x = 5.07989, Result = TRUE, Well AUTO-FLAGGEDBE1 (D113 v 7579696) x = 7.08287, Result = TRUE, Well AUTO-FLAGGEDBE2 (D113 v 7579696) x = 6.78915, Result = TRUE, Well AUTO-FLAGGEDBE3 (D398 v 7579696) x = 4.52679, Result = TRUE, Well AUTO-FLAGGEDBE4 (D398 v 7579696) x = 2.57389, Result = TRUE, Well AUTO-FLAGGEDAE5 (D77 v 7579696) x = 3.30053, Result = TRUE, Well AUTO-FLAGGEDAE6 (D77 v 7579696) x = 5.01172, Result = TRUE, Well AUTO-FLAGGEDTransform (4) Matrix ExpressionDetailsModule,C:\Program Files\Dazdaq\Manta\Transforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 888,918 bytes Created = 25 June 2004, 12:01:04 Calculations "w/w(0,1)"Input Matrices Integral () Output Matrices Control OINot Calculated&A1 (D133 v 757973E) A2 (D133 v 757973E) A3 (D398 v 757973E) A4 (D398 v 757973E) A5 (D77 v 757973E) A6 (D77 v 757973E) B1 (Standard1) B2 (Standard1) B3 (Standard2) B4 (Standard2) B5 (Standard3) B6 (Standard3) C1 (D113 v 720281J) C2 (D113 v 720281J) C3 (D398 v 720281J) C4 (D398 v 720281J) C5 (D77 v 720281J) C6 (D77 v 720281J) D1 (Standard4) D2 (Standard4) D3 (Standard5) D4 (Standard5) D5 (Standard6) D6 (Standard6) E1 (D113 v 7579696) E2 (D113 v 7579696) E3 (D398 v 7579696) E4 (D398 v 7579696) E5 (D77 v 7579696) E6 (D77 v 7579696) F1 (Standard7) F2 (Standard7) F3 (Standard8) F4 (Standard8) F5 (Standard9) F6 (Standard9) H1 (Neg Control1) H2 (Neg Control1) DetailsPlate 1 Calculations(G1 (Pos Control1) = w/w(0,1) = 4.93817(G2 (Pos Control1) = w/w(0,1) = 4.97723Group Calculations Control OID133 v 757973E)A1 = NOT INCLUDED - no result calculated)A2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D398 v 757973E)A3 = NOT INCLUDED - no result calculated)A4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. D77 v 757973E)A5 = NOT INCLUDED - no result calculated)A6 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D113 v 720281J)C1 = NOT INCLUDED - no result calculated)C2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D398 v 720281J)C3 = NOT INCLUDED - no result calculated)C4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. D77 v 720281J)C5 = NOT INCLUDED - no result calculated)C6 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D113 v 7579696)E1 = NOT INCLUDED - no result calculated)E2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D398 v 7579696)E3 = NOT INCLUDED - no result calculated)E4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. D77 v 7579696)E5 = NOT INCLUDED - no result calculated)E6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard1)B1 = NOT INCLUDED - no result calculated)B2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard2)B3 = NOT INCLUDED - no result calculated)B4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard3)B5 = NOT INCLUDED - no result calculated)B6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard4)D1 = NOT INCLUDED - no result calculated)D2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard5)D3 = NOT INCLUDED - no result calculated)D4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard6)D5 = NOT INCLUDED - no result calculated)D6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard7)F1 = NOT INCLUDED - no result calculated)F2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard8)F3 = NOT INCLUDED - no result calculated)F4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard9)F5 = NOT INCLUDED - no result calculated)F6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Pos Control1 G1 = 4.93817 G2 = 4.97723Average = 9.9154 / 2 = 4.9577 Neg Control1)H1 = NOT INCLUDED - no result calculated)H2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.#Transform (5) Validation ExpressionDetailsModule-C:\Program Files\Dazdaq\Manta\QTransforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 610,392 bytes Created = 25 June 2004, 12:01:40 CalculationsValidates: x>2.0Input MatricesControl OI () Not CalculatedDetailsPlate 1 Calculations\Could not evaluate expression: "x>2.0" - x cannot be referenced as it has not been specified2(13 October 2004 10:02:58) Calculations, version 6Raw Data Group CalculationsPlate 10nmAnalysisTransform (1) IntegralDetailsModule-C:\Program Files\Dazdaq\Manta\KRTransform.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 680,024 bytes Created = 25 June 2004, 12:01:14 CalculationseIntegrated area by trapezium method. Using all unflagged data points, Axes = (Cycle Number, Reading).Input Matrices 0nm (RLU) Output MatricesIntegralDetailsPlate 1 Calculations)NA1 (D133 v 757973E) Integral calculated by Trapezium method; Result = 232.565NA2 (D133 v 757973E) Integral calculated by Trapezium method; Result = 215.465NA3 (D398 v 757973E) Integral calculated by Trapezium method; Result = 262.646NA4 (D398 v 757973E) Integral calculated by Trapezium method; Result = 272.436MA5 (D77 v 757973E) Integral calculated by Trapezium method; Result = 287.541MA6 (D77 v 757973E) Integral calculated by Trapezium method; Result = 207.101IB1 (Standard1) Integral calculated by Trapezium method; Result = 36.5455IB2 (Standard1) Integral calculated by Trapezium method; Result = 35.7005IB3 (Standard2) Integral calculated by Trapezium method; Result = 41.3855IB4 (Standard2) Integral calculated by Trapezium method; Result = 39.8005IB5 (Standard3) Integral calculated by Trapezium method; Result = 41.4255IB6 (Standard3) Integral calculated by Trapezium method; Result = 54.4355NC1 (D113 v 720281J) Integral calculated by Trapezium method; Result = 239.041NC2 (D113 v 720281J) Integral calculated by Trapezium method; Result = 213.441NC3 (D398 v 720281J) Integral calculated by Trapezium method; Result = 201.816NC4 (D398 v 720281J) Integral calculated by Trapezium method; Result = 188.581LC5 (D77 v 720281J) Integral calculated by Trapezium method; Result = 265.72MC6 (D77 v 720281J) Integral calculated by Trapezium method; Result = 325.116ID1 (Standard4) Integral calculated by Trapezium method; Result = 161.821ID2 (Standard4) Integral calculated by Trapezium method; Result = 57.7105ID3 (Standard5) Integral calculated by Trapezium method; Result = 68.5255ID4 (Standard5) Integral calculated by Trapezium method; Result = 64.7005ID5 (Standard6) Integral calculated by Trapezium method; Result = 69.4555ID6 (Standard6) Integral calculated by Trapezium method; Result = 64.0005ME1 (D113 v 7579696) Integral calculated by Trapezium method; Result = 277.51NE2 (D113 v 7579696) Integral calculated by Trapezium method; Result = 330.465LE3 (D398 v 7579696) Integral calculated by Trapezium method; Result = 248.5NE4 (D398 v 7579696) Integral calculated by Trapezium method; Result = 135.645ME5 (D77 v 7579696) Integral calculated by Trapezium method; Result = 160.276LE6 (D77 v 7579696) Integral calculated by Trapezium method; Result = 249.31IF1 (Standard7) Integral calculated by Trapezium method; Result = 39.1805IF2 (Standard7) Integral calculated by Trapezium method; Result = 48.6755IF3 (Standard8) Integral calculated by Trapezium method; Result = 54.8955IF4 (Standard8) Integral calculated by Trapezium method; Result = 52.7005IF5 (Standard9) Integral calculated by Trapezium method; Result = 48.5605IF6 (Standard9) Integral calculated by Trapezium method; Result = 49.7455KG1 (Pos Control1) Integral calculated by Trapezium method; Result = 189.43LG2 (Pos Control1) Integral calculated by Trapezium method; Result = 221.241LH1 (Neg Control1) Integral calculated by Trapezium method; Result = 38.3605LH2 (Neg Control1) Integral calculated by Trapezium method; Result = 44.4505Group CalculationsIntegralD133 v 757973E A1 = 232.565 A2 = 215.465Average = 448.031 / 2 = 224.015D398 v 757973E A3 = 262.646 A4 = 272.436Average = 535.081 / 2 = 267.541 D77 v 757973E A5 = 287.541 A6 = 207.101Average = 494.641 / 2 = 247.321D113 v 720281J C1 = 239.041 C2 = 213.441Average = 452.481 / 2 = 226.241D398 v 720281J C3 = 201.816 C4 = 188.581Average = 390.396 / 2 = 195.198 D77 v 720281J C5 = 265.72 C6 = 325.116Average = 590.836 / 2 = 295.418D113 v 7579696 E1 = 277.51 E2 = 330.465Average = 607.976 / 2 = 303.988D398 v 7579696 E3 = 248.5 E4 = 135.645Average = 384.146 / 2 = 192.073 D77 v 7579696 E5 = 160.276 E6 = 249.31Average = 409.586 / 2 = 204.793 Standard1 B1 = 36.5455 B2 = 35.7005Average = 72.246 / 2 = 36.123 Standard2 B3 = 41.3855 B4 = 39.8005Average = 81.186 / 2 = 40.593 Standard3 B5 = 41.4255 B6 = 54.4355Average = 95.861 / 2 = 47.9305 Standard4 D1 = 161.821 D2 = 57.7105Average = 219.531 / 2 = 109.766 Standard5 D3 = 68.5255 D4 = 64.7005Average = 133.226 / 2 = 66.613 Standard6 D5 = 69.4555 D6 = 64.0005Average = 133.456 / 2 = 66.728 Standard7 F1 = 39.1805 F2 = 48.6755Average = 87.856 / 2 = 43.928 Standard8 F3 = 54.8955 F4 = 52.7005Average = 107.596 / 2 = 53.798 Standard9 F5 = 48.5605 F6 = 49.7455Average = 98.306 / 2 = 49.153 Pos Control1 G1 = 189.43 G2 = 221.241Average = 410.671 / 2 = 205.335 Neg Control1 H1 = 38.3605 H2 = 44.4505Average = 82.811 / 2 = 41.4055Transform (2) Matrix ExpressionDetailsModule,C:\Program Files\Dazdaq\Manta\Transforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 888,918 bytes Created = 25 June 2004, 12:01:04 Calculations "w/w(0,1)"Input Matrices Integral () Output Matrices Opsonic IndexNot CalculatedB1 (Standard1) B2 (Standard1) B3 (Standard2) B4 (Standard2) B5 (Standard3) B6 (Standard3) D1 (Standard4) D2 (Standard4) D3 (Standard5) D4 (Standard5) D5 (Standard6) D6 (Standard6) F1 (Standard7) F2 (Standard7) F3 (Standard8) F4 (Standard8) F5 (Standard9) F6 (Standard9) DetailsPlate 1 Calculations*A1 (D133 v 757973E) = w/w(0,1) = 6.36372*A2 (D133 v 757973E) = w/w(0,1) = 6.03536*A3 (D398 v 757973E) = w/w(0,1) = 6.34632*A4 (D398 v 757973E) = w/w(0,1) = 6.84503)A5 (D77 v 757973E) = w/w(0,1) = 6.94115)A6 (D77 v 757973E) = w/w(0,1) = 3.80451)C1 (D113 v 720281J) = w/w(0,1) = 1.4772*C2 (D113 v 720281J) = w/w(0,1) = 3.69847*C3 (D398 v 720281J) = w/w(0,1) = 2.94512*C4 (D398 v 720281J) = w/w(0,1) = 2.91467)C5 (D77 v 720281J) = w/w(0,1) = 3.82577)C6 (D77 v 720281J) = w/w(0,1) = 5.07989*E1 (D113 v 7579696) = w/w(0,1) = 7.08287*E2 (D113 v 7579696) = w/w(0,1) = 6.78915*E3 (D398 v 7579696) = w/w(0,1) = 4.52679*E4 (D398 v 7579696) = w/w(0,1) = 2.57389)E5 (D77 v 7579696) = w/w(0,1) = 3.30053)E6 (D77 v 7579696) = w/w(0,1) = 5.01172(G1 (Pos Control1) = w/w(0,1) = 4.93817(G2 (Pos Control1) = w/w(0,1) = 4.97723WH1 (Neg Control1) Could not evaluate: "w/w(0,1)" - Well offset out of microplate rangeWH2 (Neg Control1) Could not evaluate: "w/w(0,1)" - Well offset out of microplate rangeGroup Calculations Opsonic IndexD133 v 757973E A1 = 6.36372 A2 = 6.03536Average = 12.3991 / 2 = 6.19954D398 v 757973E A3 = 6.34632 A4 = 6.84503Average = 13.1913 / 2 = 6.59567 D77 v 757973E A5 = 6.94115 A6 = 3.80451Average = 10.7457 / 2 = 5.37283D113 v 720281J C1 = 1.4772 C2 = 3.69847Average = 5.17566 / 2 = 2.58783D398 v 720281J C3 = 2.94512 C4 = 2.91467Average = 5.85978 / 2 = 2.92989 D77 v 720281J C5 = 3.82577 C6 = 5.07989Average = 8.90566 / 2 = 4.45283D113 v 7579696 E1 = 7.08287 E2 = 6.78915Average = 13.872 / 2 = 6.93601D398 v 7579696 E3 = 4.52679 E4 = 2.57389Average = 7.10069 / 2 = 3.55034 D77 v 7579696 E5 = 3.30053 E6 = 5.01172Average = 8.31225 / 2 = 4.15613 Standard1)B1 = NOT INCLUDED - no result calculated)B2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard2)B3 = NOT INCLUDED - no result calculated)B4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard3)B5 = NOT INCLUDED - no result calculated)B6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard4)D1 = NOT INCLUDED - no result calculated)D2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard5)D3 = NOT INCLUDED - no result calculated)D4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard6)D5 = NOT INCLUDED - no result calculated)D6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard7)F1 = NOT INCLUDED - no result calculated)F2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard8)F3 = NOT INCLUDED - no result calculated)F4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard9)F5 = NOT INCLUDED - no result calculated)F6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Pos Control1 G1 = 4.93817 G2 = 4.97723Average = 9.9154 / 2 = 4.9577 Neg Control1&H1 = NOT INCLUDED - calculation error&H2 = NOT INCLUDED - calculation error&All wells in this group were excluded.Transform (3) Auto Flag By WellDetailsModule,C:\Program Files\Dazdaq\Manta\Transforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 888,918 bytes Created = 25 June 2004, 12:01:04 Calculationsx>1.2Input MatricesOpsonic Index () Not CalculatedB1 (Standard1) B2 (Standard1) B3 (Standard2) B4 (Standard2) B5 (Standard3) B6 (Standard3) D1 (Standard4) D2 (Standard4) D3 (Standard5) D4 (Standard5) D5 (Standard6) D6 (Standard6) F1 (Standard7) F2 (Standard7) F3 (Standard8) F4 (Standard8) F5 (Standard9) F6 (Standard9) G1 (Pos Control1) G2 (Pos Control1) H1 (Neg Control1) H2 (Neg Control1) DetailsPlate 1 CalculationsExpression = x>1.2BA1 (D133 v 757973E) x = 6.36372, Result = TRUE, Well AUTO-FLAGGEDBA2 (D133 v 757973E) x = 6.03536, Result = TRUE, Well AUTO-FLAGGEDBA3 (D398 v 757973E) x = 6.34632, Result = TRUE, Well AUTO-FLAGGEDBA4 (D398 v 757973E) x = 6.84503, Result = TRUE, Well AUTO-FLAGGEDAA5 (D77 v 757973E) x = 6.94115, Result = TRUE, Well AUTO-FLAGGEDAA6 (D77 v 757973E) x = 3.80451, Result = TRUE, Well AUTO-FLAGGEDAC1 (D113 v 720281J) x = 1.4772, Result = TRUE, Well AUTO-FLAGGEDBC2 (D113 v 720281J) x = 3.69847, Result = TRUE, Well AUTO-FLAGGEDBC3 (D398 v 720281J) x = 2.94512, Result = TRUE, Well AUTO-FLAGGEDBC4 (D398 v 720281J) x = 2.91467, Result = TRUE, Well AUTO-FLAGGEDAC5 (D77 v 720281J) x = 3.82577, Result = TRUE, Well AUTO-FLAGGEDAC6 (D77 v 720281J) x = 5.07989, Result = TRUE, Well AUTO-FLAGGEDBE1 (D113 v 7579696) x = 7.08287, Result = TRUE, Well AUTO-FLAGGEDBE2 (D113 v 7579696) x = 6.78915, Result = TRUE, Well AUTO-FLAGGEDBE3 (D398 v 7579696) x = 4.52679, Result = TRUE, Well AUTO-FLAGGEDBE4 (D398 v 7579696) x = 2.57389, Result = TRUE, Well AUTO-FLAGGEDAE5 (D77 v 7579696) x = 3.30053, Result = TRUE, Well AUTO-FLAGGEDAE6 (D77 v 7579696) x = 5.01172, Result = TRUE, Well AUTO-FLAGGEDTransform (4) Matrix ExpressionDetailsModule,C:\Program Files\Dazdaq\Manta\Transforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 888,918 bytes Created = 25 June 2004, 12:01:04 Calculations "w/w(0,1)"Input Matrices Integral () Output Matrices Control OINot Calculated&A1 (D133 v 757973E) A2 (D133 v 757973E) A3 (D398 v 757973E) A4 (D398 v 757973E) A5 (D77 v 757973E) A6 (D77 v 757973E) B1 (Standard1) B2 (Standard1) B3 (Standard2) B4 (Standard2) B5 (Standard3) B6 (Standard3) C1 (D113 v 720281J) C2 (D113 v 720281J) C3 (D398 v 720281J) C4 (D398 v 720281J) C5 (D77 v 720281J) C6 (D77 v 720281J) D1 (Standard4) D2 (Standard4) D3 (Standard5) D4 (Standard5) D5 (Standard6) D6 (Standard6) E1 (D113 v 7579696) E2 (D113 v 7579696) E3 (D398 v 7579696) E4 (D398 v 7579696) E5 (D77 v 7579696) E6 (D77 v 7579696) F1 (Standard7) F2 (Standard7) F3 (Standard8) F4 (Standard8) F5 (Standard9) F6 (Standard9) H1 (Neg Control1) H2 (Neg Control1) DetailsPlate 1 Calculations(G1 (Pos Control1) = w/w(0,1) = 4.93817(G2 (Pos Control1) = w/w(0,1) = 4.97723Group Calculations Control OID133 v 757973E)A1 = NOT INCLUDED - no result calculated)A2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D398 v 757973E)A3 = NOT INCLUDED - no result calculated)A4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. D77 v 757973E)A5 = NOT INCLUDED - no result calculated)A6 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D113 v 720281J)C1 = NOT INCLUDED - no result calculated)C2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D398 v 720281J)C3 = NOT INCLUDED - no result calculated)C4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. D77 v 720281J)C5 = NOT INCLUDED - no result calculated)C6 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D113 v 7579696)E1 = NOT INCLUDED - no result calculated)E2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D398 v 7579696)E3 = NOT INCLUDED - no result calculated)E4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. D77 v 7579696)E5 = NOT INCLUDED - no result calculated)E6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard1)B1 = NOT INCLUDED - no result calculated)B2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard2)B3 = NOT INCLUDED - no result calculated)B4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard3)B5 = NOT INCLUDED - no result calculated)B6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard4)D1 = NOT INCLUDED - no result calculated)D2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard5)D3 = NOT INCLUDED - no result calculated)D4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard6)D5 = NOT INCLUDED - no result calculated)D6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard7)F1 = NOT INCLUDED - no result calculated)F2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard8)F3 = NOT INCLUDED - no result calculated)F4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard9)F5 = NOT INCLUDED - no result calculated)F6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Pos Control1 G1 = 4.93817 G2 = 4.97723Average = 9.9154 / 2 = 4.9577 Neg Control1)H1 = NOT INCLUDED - no result calculated)H2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.#Transform (5) Validation ExpressionDetailsModule-C:\Program Files\Dazdaq\Manta\QTransforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 610,392 bytes Created = 25 June 2004, 12:01:40 CalculationsValidates: x>2.0Input MatricesControl OI () Not CalculatedDetailsPlate 1 Calculations\Could not evaluate expression: "x>2.0" - x cannot be referenced as it has not been specified2(13 October 2004 10:05:25) Calculations, version 7Raw Data Group CalculationsPlate 10nmAnalysisTransform (1) IntegralDetailsModule-C:\Program Files\Dazdaq\Manta\KRTransform.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 680,024 bytes Created = 25 June 2004, 12:01:14 CalculationseIntegrated area by trapezium method. Using all unflagged data points, Axes = (Cycle Number, Reading).Input Matrices 0nm (RLU) Output MatricesIntegralDetailsPlate 1 Calculations)NA1 (D133 v 757973E) Integral calculated by Trapezium method; Result = 232.565NA2 (D133 v 757973E) Integral calculated by Trapezium method; Result = 215.465NA3 (D398 v 757973E) Integral calculated by Trapezium method; Result = 262.646NA4 (D398 v 757973E) Integral calculated by Trapezium method; Result = 272.436MA5 (D77 v 757973E) Integral calculated by Trapezium method; Result = 287.541MA6 (D77 v 757973E) Integral calculated by Trapezium method; Result = 207.101IB1 (Standard1) Integral calculated by Trapezium method; Result = 36.5455IB2 (Standard1) Integral calculated by Trapezium method; Result = 35.7005IB3 (Standard2) Integral calculated by Trapezium method; Result = 41.3855IB4 (Standard2) Integral calculated by Trapezium method; Result = 39.8005IB5 (Standard3) Integral calculated by Trapezium method; Result = 41.4255IB6 (Standard3) Integral calculated by Trapezium method; Result = 54.4355NC1 (D113 v 720281J) Integral calculated by Trapezium method; Result = 239.041NC2 (D113 v 720281J) Integral calculated by Trapezium method; Result = 213.441NC3 (D398 v 720281J) Integral calculated by Trapezium method; Result = 201.816NC4 (D398 v 720281J) Integral calculated by Trapezium method; Result = 188.581LC5 (D77 v 720281J) Integral calculated by Trapezium method; Result = 265.72MC6 (D77 v 720281J) Integral calculated by Trapezium method; Result = 325.116ID1 (Standard4) Integral calculated by Trapezium method; Result = 161.821ID2 (Standard4) Integral calculated by Trapezium method; Result = 57.7105ID3 (Standard5) Integral calculated by Trapezium method; Result = 68.5255ID4 (Standard5) Integral calculated by Trapezium method; Result = 64.7005ID5 (Standard6) Integral calculated by Trapezium method; Result = 69.4555ID6 (Standard6) Integral calculated by Trapezium method; Result = 64.0005ME1 (D113 v 7579696) Integral calculated by Trapezium method; Result = 277.51NE2 (D113 v 7579696) Integral calculated by Trapezium method; Result = 330.465LE3 (D398 v 7579696) Integral calculated by Trapezium method; Result = 248.5NE4 (D398 v 7579696) Integral calculated by Trapezium method; Result = 135.645ME5 (D77 v 7579696) Integral calculated by Trapezium method; Result = 160.276LE6 (D77 v 7579696) Integral calculated by Trapezium method; Result = 249.31IF1 (Standard7) Integral calculated by Trapezium method; Result = 39.1805IF2 (Standard7) Integral calculated by Trapezium method; Result = 48.6755IF3 (Standard8) Integral calculated by Trapezium method; Result = 54.8955IF4 (Standard8) Integral calculated by Trapezium method; Result = 52.7005IF5 (Standard9) Integral calculated by Trapezium method; Result = 48.5605IF6 (Standard9) Integral calculated by Trapezium method; Result = 49.7455KG1 (Pos Control1) Integral calculated by Trapezium method; Result = 189.43LG2 (Pos Control1) Integral calculated by Trapezium method; Result = 221.241LH1 (Neg Control1) Integral calculated by Trapezium method; Result = 38.3605LH2 (Neg Control1) Integral calculated by Trapezium method; Result = 44.4505Group CalculationsIntegralD133 v 757973E A1 = 232.565 A2 = 215.465Average = 448.031 / 2 = 224.015D398 v 757973E A3 = 262.646 A4 = 272.436Average = 535.081 / 2 = 267.541 D77 v 757973E A5 = 287.541 A6 = 207.101Average = 494.641 / 2 = 247.321D113 v 720281J C1 = 239.041 C2 = 213.441Average = 452.481 / 2 = 226.241D398 v 720281J C3 = 201.816 C4 = 188.581Average = 390.396 / 2 = 195.198 D77 v 720281J C5 = 265.72 C6 = 325.116Average = 590.836 / 2 = 295.418D113 v 7579696 E1 = 277.51 E2 = 330.465Average = 607.976 / 2 = 303.988D398 v 7579696 E3 = 248.5 E4 = 135.645Average = 384.146 / 2 = 192.073 D77 v 7579696 E5 = 160.276 E6 = 249.31Average = 409.586 / 2 = 204.793 Standard1 B1 = 36.5455 B2 = 35.7005Average = 72.246 / 2 = 36.123 Standard2 B3 = 41.3855 B4 = 39.8005Average = 81.186 / 2 = 40.593 Standard3 B5 = 41.4255 B6 = 54.4355Average = 95.861 / 2 = 47.9305 Standard4 D1 = 161.821 D2 = 57.7105Average = 219.531 / 2 = 109.766 Standard5 D3 = 68.5255 D4 = 64.7005Average = 133.226 / 2 = 66.613 Standard6 D5 = 69.4555 D6 = 64.0005Average = 133.456 / 2 = 66.728 Standard7 F1 = 39.1805 F2 = 48.6755Average = 87.856 / 2 = 43.928 Standard8 F3 = 54.8955 F4 = 52.7005Average = 107.596 / 2 = 53.798 Standard9 F5 = 48.5605 F6 = 49.7455Average = 98.306 / 2 = 49.153 Pos Control1 G1 = 189.43 G2 = 221.241Average = 410.671 / 2 = 205.335 Neg Control1 H1 = 38.3605 H2 = 44.4505Average = 82.811 / 2 = 41.4055Transform (2) Matrix ExpressionDetailsModule,C:\Program Files\Dazdaq\Manta\Transforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 888,918 bytes Created = 25 June 2004, 12:01:04 Calculations "w/w(0,1)"Input Matrices Integral () Output Matrices Opsonic IndexNot CalculatedB1 (Standard1) B2 (Standard1) B3 (Standard2) B4 (Standard2) B5 (Standard3) B6 (Standard3) D1 (Standard4) D2 (Standard4) D3 (Standard5) D4 (Standard5) D5 (Standard6) D6 (Standard6) F1 (Standard7) F2 (Standard7) F3 (Standard8) F4 (Standard8) F5 (Standard9) F6 (Standard9) DetailsPlate 1 Calculations*A1 (D133 v 757973E) = w/w(0,1) = 6.36372*A2 (D133 v 757973E) = w/w(0,1) = 6.03536*A3 (D398 v 757973E) = w/w(0,1) = 6.34632*A4 (D398 v 757973E) = w/w(0,1) = 6.84503)A5 (D77 v 757973E) = w/w(0,1) = 6.94115)A6 (D77 v 757973E) = w/w(0,1) = 3.80451)C1 (D113 v 720281J) = w/w(0,1) = 1.4772*C2 (D113 v 720281J) = w/w(0,1) = 3.69847*C3 (D398 v 720281J) = w/w(0,1) = 2.94512*C4 (D398 v 720281J) = w/w(0,1) = 2.91467)C5 (D77 v 720281J) = w/w(0,1) = 3.82577)C6 (D77 v 720281J) = w/w(0,1) = 5.07989*E1 (D113 v 7579696) = w/w(0,1) = 7.08287*E2 (D113 v 7579696) = w/w(0,1) = 6.78915*E3 (D398 v 7579696) = w/w(0,1) = 4.52679*E4 (D398 v 7579696) = w/w(0,1) = 2.57389)E5 (D77 v 7579696) = w/w(0,1) = 3.30053)E6 (D77 v 7579696) = w/w(0,1) = 5.01172(G1 (Pos Control1) = w/w(0,1) = 4.93817(G2 (Pos Control1) = w/w(0,1) = 4.97723WH1 (Neg Control1) Could not evaluate: "w/w(0,1)" - Well offset out of microplate rangeWH2 (Neg Control1) Could not evaluate: "w/w(0,1)" - Well offset out of microplate rangeGroup Calculations Opsonic IndexD133 v 757973E A1 = 6.36372 A2 = 6.03536Average = 12.3991 / 2 = 6.19954D398 v 757973E A3 = 6.34632 A4 = 6.84503Average = 13.1913 / 2 = 6.59567 D77 v 757973E A5 = 6.94115 A6 = 3.80451Average = 10.7457 / 2 = 5.37283D113 v 720281J C1 = 1.4772 C2 = 3.69847Average = 5.17566 / 2 = 2.58783D398 v 720281J C3 = 2.94512 C4 = 2.91467Average = 5.85978 / 2 = 2.92989 D77 v 720281J C5 = 3.82577 C6 = 5.07989Average = 8.90566 / 2 = 4.45283D113 v 7579696 E1 = 7.08287 E2 = 6.78915Average = 13.872 / 2 = 6.93601D398 v 7579696 E3 = 4.52679 E4 = 2.57389Average = 7.10069 / 2 = 3.55034 D77 v 7579696 E5 = 3.30053 E6 = 5.01172Average = 8.31225 / 2 = 4.15613 Standard1)B1 = NOT INCLUDED - no result calculated)B2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard2)B3 = NOT INCLUDED - no result calculated)B4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard3)B5 = NOT INCLUDED - no result calculated)B6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard4)D1 = NOT INCLUDED - no result calculated)D2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard5)D3 = NOT INCLUDED - no result calculated)D4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard6)D5 = NOT INCLUDED - no result calculated)D6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard7)F1 = NOT INCLUDED - no result calculated)F2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard8)F3 = NOT INCLUDED - no result calculated)F4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard9)F5 = NOT INCLUDED - no result calculated)F6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Pos Control1 G1 = 4.93817 G2 = 4.97723Average = 9.9154 / 2 = 4.9577 Neg Control1&H1 = NOT INCLUDED - calculation error&H2 = NOT INCLUDED - calculation error&All wells in this group were excluded.Transform (3) Auto Flag By WellDetailsModule,C:\Program Files\Dazdaq\Manta\Transforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 888,918 bytes Created = 25 June 2004, 12:01:04 Calculationsx>1.2Input MatricesOpsonic Index () Not CalculatedB1 (Standard1) B2 (Standard1) B3 (Standard2) B4 (Standard2) B5 (Standard3) B6 (Standard3) D1 (Standard4) D2 (Standard4) D3 (Standard5) D4 (Standard5) D5 (Standard6) D6 (Standard6) F1 (Standard7) F2 (Standard7) F3 (Standard8) F4 (Standard8) F5 (Standard9) F6 (Standard9) G1 (Pos Control1) G2 (Pos Control1) H1 (Neg Control1) H2 (Neg Control1) DetailsPlate 1 CalculationsExpression = x>1.2BA1 (D133 v 757973E) x = 6.36372, Result = TRUE, Well AUTO-FLAGGEDBA2 (D133 v 757973E) x = 6.03536, Result = TRUE, Well AUTO-FLAGGEDBA3 (D398 v 757973E) x = 6.34632, Result = TRUE, Well AUTO-FLAGGEDBA4 (D398 v 757973E) x = 6.84503, Result = TRUE, Well AUTO-FLAGGEDAA5 (D77 v 757973E) x = 6.94115, Result = TRUE, Well AUTO-FLAGGEDAA6 (D77 v 757973E) x = 3.80451, Result = TRUE, Well AUTO-FLAGGEDAC1 (D113 v 720281J) x = 1.4772, Result = TRUE, Well AUTO-FLAGGEDBC2 (D113 v 720281J) x = 3.69847, Result = TRUE, Well AUTO-FLAGGEDBC3 (D398 v 720281J) x = 2.94512, Result = TRUE, Well AUTO-FLAGGEDBC4 (D398 v 720281J) x = 2.91467, Result = TRUE, Well AUTO-FLAGGEDAC5 (D77 v 720281J) x = 3.82577, Result = TRUE, Well AUTO-FLAGGEDAC6 (D77 v 720281J) x = 5.07989, Result = TRUE, Well AUTO-FLAGGEDBE1 (D113 v 7579696) x = 7.08287, Result = TRUE, Well AUTO-FLAGGEDBE2 (D113 v 7579696) x = 6.78915, Result = TRUE, Well AUTO-FLAGGEDBE3 (D398 v 7579696) x = 4.52679, Result = TRUE, Well AUTO-FLAGGEDBE4 (D398 v 7579696) x = 2.57389, Result = TRUE, Well AUTO-FLAGGEDAE5 (D77 v 7579696) x = 3.30053, Result = TRUE, Well AUTO-FLAGGEDAE6 (D77 v 7579696) x = 5.01172, Result = TRUE, Well AUTO-FLAGGEDTransform (4) Matrix ExpressionDetailsModule,C:\Program Files\Dazdaq\Manta\Transforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 888,918 bytes Created = 25 June 2004, 12:01:04 Calculations "w/w(0,1)"Input Matrices Integral () Output Matrices Control OINot Calculated&A1 (D133 v 757973E) A2 (D133 v 757973E) A3 (D398 v 757973E) A4 (D398 v 757973E) A5 (D77 v 757973E) A6 (D77 v 757973E) B1 (Standard1) B2 (Standard1) B3 (Standard2) B4 (Standard2) B5 (Standard3) B6 (Standard3) C1 (D113 v 720281J) C2 (D113 v 720281J) C3 (D398 v 720281J) C4 (D398 v 720281J) C5 (D77 v 720281J) C6 (D77 v 720281J) D1 (Standard4) D2 (Standard4) D3 (Standard5) D4 (Standard5) D5 (Standard6) D6 (Standard6) E1 (D113 v 7579696) E2 (D113 v 7579696) E3 (D398 v 7579696) E4 (D398 v 7579696) E5 (D77 v 7579696) E6 (D77 v 7579696) F1 (Standard7) F2 (Standard7) F3 (Standard8) F4 (Standard8) F5 (Standard9) F6 (Standard9) H1 (Neg Control1) H2 (Neg Control1) DetailsPlate 1 Calculations(G1 (Pos Control1) = w/w(0,1) = 4.93817(G2 (Pos Control1) = w/w(0,1) = 4.97723Group Calculations Control OID133 v 757973E)A1 = NOT INCLUDED - no result calculated)A2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D398 v 757973E)A3 = NOT INCLUDED - no result calculated)A4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. D77 v 757973E)A5 = NOT INCLUDED - no result calculated)A6 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D113 v 720281J)C1 = NOT INCLUDED - no result calculated)C2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D398 v 720281J)C3 = NOT INCLUDED - no result calculated)C4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. D77 v 720281J)C5 = NOT INCLUDED - no result calculated)C6 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D113 v 7579696)E1 = NOT INCLUDED - no result calculated)E2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D398 v 7579696)E3 = NOT INCLUDED - no result calculated)E4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. D77 v 7579696)E5 = NOT INCLUDED - no result calculated)E6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard1)B1 = NOT INCLUDED - no result calculated)B2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard2)B3 = NOT INCLUDED - no result calculated)B4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard3)B5 = NOT INCLUDED - no result calculated)B6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard4)D1 = NOT INCLUDED - no result calculated)D2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard5)D3 = NOT INCLUDED - no result calculated)D4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard6)D5 = NOT INCLUDED - no result calculated)D6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard7)F1 = NOT INCLUDED - no result calculated)F2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard8)F3 = NOT INCLUDED - no result calculated)F4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard9)F5 = NOT INCLUDED - no result calculated)F6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Pos Control1 G1 = 4.93817 G2 = 4.97723Average = 9.9154 / 2 = 4.9577 Neg Control1)H1 = NOT INCLUDED - no result calculated)H2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.#Transform (5) Validation ExpressionDetailsModule-C:\Program Files\Dazdaq\Manta\QTransforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 610,392 bytes Created = 25 June 2004, 12:01:40 CalculationsValidates: x>2.0Input MatricesControl OI () Not CalculatedDetailsPlate 1 Calculations\Could not evaluate expression: "x>2.0" - x cannot be referenced as it has not been specifiedTransform (6) Auto Flag By WellDetailsModule,C:\Program Files\Dazdaq\Manta\Transforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 888,918 bytes Created = 25 June 2004, 12:01:04 Calculationsx >2.0Input MatricesControl OI () Not Calculated&A1 (D133 v 757973E) A2 (D133 v 757973E) A3 (D398 v 757973E) A4 (D398 v 757973E) A5 (D77 v 757973E) A6 (D77 v 757973E) B1 (Standard1) B2 (Standard1) B3 (Standard2) B4 (Standard2) B5 (Standard3) B6 (Standard3) C1 (D113 v 720281J) C2 (D113 v 720281J) C3 (D398 v 720281J) C4 (D398 v 720281J) C5 (D77 v 720281J) C6 (D77 v 720281J) D1 (Standard4) D2 (Standard4) D3 (Standard5) D4 (Standard5) D5 (Standard6) D6 (Standard6) E1 (D113 v 7579696) E2 (D113 v 7579696) E3 (D398 v 7579696) E4 (D398 v 7579696) E5 (D77 v 7579696) E6 (D77 v 7579696) F1 (Standard7) F2 (Standard7) F3 (Standard8) F4 (Standard8) F5 (Standard9) F6 (Standard9) H1 (Neg Control1) H2 (Neg Control1) DetailsPlate 1 CalculationsExpression = x >2.0@G1 (Pos Control1) x = 4.93817, Result = TRUE, Well AUTO-FLAGGED@G2 (Pos Control1) x = 4.97723, Result = TRUE, Well AUTO-FLAGGED2(13 October 2004 10:09:13) Calculations, version 8Raw Data Group CalculationsPlate 10nmAnalysisTransform (1) IntegralDetailsModule-C:\Program Files\Dazdaq\Manta\KRTransform.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 680,024 bytes Created = 25 June 2004, 12:01:14 CalculationseIntegrated area by trapezium method. Using all unflagged data points, Axes = (Cycle Number, Reading).Input Matrices 0nm (RLU) Output MatricesIntegralDetailsPlate 1 Calculations)NA1 (D133 v 757973E) Integral calculated by Trapezium method; Result = 232.565NA2 (D133 v 757973E) Integral calculated by Trapezium method; Result = 215.465NA3 (D398 v 757973E) Integral calculated by Trapezium method; Result = 262.646NA4 (D398 v 757973E) Integral calculated by Trapezium method; Result = 272.436MA5 (D77 v 757973E) Integral calculated by Trapezium method; Result = 287.541MA6 (D77 v 757973E) Integral calculated by Trapezium method; Result = 207.101IB1 (Standard1) Integral calculated by Trapezium method; Result = 36.5455IB2 (Standard1) Integral calculated by Trapezium method; Result = 35.7005IB3 (Standard2) Integral calculated by Trapezium method; Result = 41.3855IB4 (Standard2) Integral calculated by Trapezium method; Result = 39.8005IB5 (Standard3) Integral calculated by Trapezium method; Result = 41.4255IB6 (Standard3) Integral calculated by Trapezium method; Result = 54.4355NC1 (D113 v 720281J) Integral calculated by Trapezium method; Result = 239.041NC2 (D113 v 720281J) Integral calculated by Trapezium method; Result = 213.441NC3 (D398 v 720281J) Integral calculated by Trapezium method; Result = 201.816NC4 (D398 v 720281J) Integral calculated by Trapezium method; Result = 188.581LC5 (D77 v 720281J) Integral calculated by Trapezium method; Result = 265.72MC6 (D77 v 720281J) Integral calculated by Trapezium method; Result = 325.116ID1 (Standard4) Integral calculated by Trapezium method; Result = 161.821ID2 (Standard4) Integral calculated by Trapezium method; Result = 57.7105ID3 (Standard5) Integral calculated by Trapezium method; Result = 68.5255ID4 (Standard5) Integral calculated by Trapezium method; Result = 64.7005ID5 (Standard6) Integral calculated by Trapezium method; Result = 69.4555ID6 (Standard6) Integral calculated by Trapezium method; Result = 64.0005ME1 (D113 v 7579696) Integral calculated by Trapezium method; Result = 277.51NE2 (D113 v 7579696) Integral calculated by Trapezium method; Result = 330.465LE3 (D398 v 7579696) Integral calculated by Trapezium method; Result = 248.5NE4 (D398 v 7579696) Integral calculated by Trapezium method; Result = 135.645ME5 (D77 v 7579696) Integral calculated by Trapezium method; Result = 160.276LE6 (D77 v 7579696) Integral calculated by Trapezium method; Result = 249.31IF1 (Standard7) Integral calculated by Trapezium method; Result = 39.1805IF2 (Standard7) Integral calculated by Trapezium method; Result = 48.6755IF3 (Standard8) Integral calculated by Trapezium method; Result = 54.8955IF4 (Standard8) Integral calculated by Trapezium method; Result = 52.7005IF5 (Standard9) Integral calculated by Trapezium method; Result = 48.5605IF6 (Standard9) Integral calculated by Trapezium method; Result = 49.7455KG1 (Pos Control1) Integral calculated by Trapezium method; Result = 189.43LG2 (Pos Control1) Integral calculated by Trapezium method; Result = 221.241LH1 (Neg Control1) Integral calculated by Trapezium method; Result = 38.3605LH2 (Neg Control1) Integral calculated by Trapezium method; Result = 44.4505Group CalculationsIntegralD133 v 757973E A1 = 232.565 A2 = 215.465Average = 448.031 / 2 = 224.015D398 v 757973E A3 = 262.646 A4 = 272.436Average = 535.081 / 2 = 267.541 D77 v 757973E A5 = 287.541 A6 = 207.101Average = 494.641 / 2 = 247.321D113 v 720281J C1 = 239.041 C2 = 213.441Average = 452.481 / 2 = 226.241D398 v 720281J C3 = 201.816 C4 = 188.581Average = 390.396 / 2 = 195.198 D77 v 720281J C5 = 265.72 C6 = 325.116Average = 590.836 / 2 = 295.418D113 v 7579696 E1 = 277.51 E2 = 330.465Average = 607.976 / 2 = 303.988D398 v 7579696 E3 = 248.5 E4 = 135.645Average = 384.146 / 2 = 192.073 D77 v 7579696 E5 = 160.276 E6 = 249.31Average = 409.586 / 2 = 204.793 Standard1 B1 = 36.5455 B2 = 35.7005Average = 72.246 / 2 = 36.123 Standard2 B3 = 41.3855 B4 = 39.8005Average = 81.186 / 2 = 40.593 Standard3 B5 = 41.4255 B6 = 54.4355Average = 95.861 / 2 = 47.9305 Standard4 D1 = 161.821 D2 = 57.7105Average = 219.531 / 2 = 109.766 Standard5 D3 = 68.5255 D4 = 64.7005Average = 133.226 / 2 = 66.613 Standard6 D5 = 69.4555 D6 = 64.0005Average = 133.456 / 2 = 66.728 Standard7 F1 = 39.1805 F2 = 48.6755Average = 87.856 / 2 = 43.928 Standard8 F3 = 54.8955 F4 = 52.7005Average = 107.596 / 2 = 53.798 Standard9 F5 = 48.5605 F6 = 49.7455Average = 98.306 / 2 = 49.153 Pos Control1 G1 = 189.43 G2 = 221.241Average = 410.671 / 2 = 205.335 Neg Control1 H1 = 38.3605 H2 = 44.4505Average = 82.811 / 2 = 41.4055Transform (2) Matrix ExpressionDetailsModule,C:\Program Files\Dazdaq\Manta\Transforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 888,918 bytes Created = 25 June 2004, 12:01:04 Calculations "w/w(0,1)"Input Matrices Integral () Output Matrices Opsonic IndexNot CalculatedB1 (Standard1) B2 (Standard1) B3 (Standard2) B4 (Standard2) B5 (Standard3) B6 (Standard3) D1 (Standard4) D2 (Standard4) D3 (Standard5) D4 (Standard5) D5 (Standard6) D6 (Standard6) F1 (Standard7) F2 (Standard7) F3 (Standard8) F4 (Standard8) F5 (Standard9) F6 (Standard9) DetailsPlate 1 Calculations*A1 (D133 v 757973E) = w/w(0,1) = 6.36372*A2 (D133 v 757973E) = w/w(0,1) = 6.03536*A3 (D398 v 757973E) = w/w(0,1) = 6.34632*A4 (D398 v 757973E) = w/w(0,1) = 6.84503)A5 (D77 v 757973E) = w/w(0,1) = 6.94115)A6 (D77 v 757973E) = w/w(0,1) = 3.80451)C1 (D113 v 720281J) = w/w(0,1) = 1.4772*C2 (D113 v 720281J) = w/w(0,1) = 3.69847*C3 (D398 v 720281J) = w/w(0,1) = 2.94512*C4 (D398 v 720281J) = w/w(0,1) = 2.91467)C5 (D77 v 720281J) = w/w(0,1) = 3.82577)C6 (D77 v 720281J) = w/w(0,1) = 5.07989*E1 (D113 v 7579696) = w/w(0,1) = 7.08287*E2 (D113 v 7579696) = w/w(0,1) = 6.78915*E3 (D398 v 7579696) = w/w(0,1) = 4.52679*E4 (D398 v 7579696) = w/w(0,1) = 2.57389)E5 (D77 v 7579696) = w/w(0,1) = 3.30053)E6 (D77 v 7579696) = w/w(0,1) = 5.01172(G1 (Pos Control1) = w/w(0,1) = 4.93817(G2 (Pos Control1) = w/w(0,1) = 4.97723WH1 (Neg Control1) Could not evaluate: "w/w(0,1)" - Well offset out of microplate rangeWH2 (Neg Control1) Could not evaluate: "w/w(0,1)" - Well offset out of microplate rangeGroup Calculations Opsonic IndexD133 v 757973E A1 = 6.36372 A2 = 6.03536Average = 12.3991 / 2 = 6.19954D398 v 757973E A3 = 6.34632 A4 = 6.84503Average = 13.1913 / 2 = 6.59567 D77 v 757973E A5 = 6.94115 A6 = 3.80451Average = 10.7457 / 2 = 5.37283D113 v 720281J C1 = 1.4772 C2 = 3.69847Average = 5.17566 / 2 = 2.58783D398 v 720281J C3 = 2.94512 C4 = 2.91467Average = 5.85978 / 2 = 2.92989 D77 v 720281J C5 = 3.82577 C6 = 5.07989Average = 8.90566 / 2 = 4.45283D113 v 7579696 E1 = 7.08287 E2 = 6.78915Average = 13.872 / 2 = 6.93601D398 v 7579696 E3 = 4.52679 E4 = 2.57389Average = 7.10069 / 2 = 3.55034 D77 v 7579696 E5 = 3.30053 E6 = 5.01172Average = 8.31225 / 2 = 4.15613 Standard1)B1 = NOT INCLUDED - no result calculated)B2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard2)B3 = NOT INCLUDED - no result calculated)B4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard3)B5 = NOT INCLUDED - no result calculated)B6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard4)D1 = NOT INCLUDED - no result calculated)D2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard5)D3 = NOT INCLUDED - no result calculated)D4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard6)D5 = NOT INCLUDED - no result calculated)D6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard7)F1 = NOT INCLUDED - no result calculated)F2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard8)F3 = NOT INCLUDED - no result calculated)F4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard9)F5 = NOT INCLUDED - no result calculated)F6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Pos Control1 G1 = 4.93817 G2 = 4.97723Average = 9.9154 / 2 = 4.9577 Neg Control1&H1 = NOT INCLUDED - calculation error&H2 = NOT INCLUDED - calculation error&All wells in this group were excluded.Transform (3) Auto Flag By WellDetailsModule,C:\Program Files\Dazdaq\Manta\Transforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 888,918 bytes Created = 25 June 2004, 12:01:04 Calculationsx>1.2Input MatricesOpsonic Index () Not CalculatedB1 (Standard1) B2 (Standard1) B3 (Standard2) B4 (Standard2) B5 (Standard3) B6 (Standard3) D1 (Standard4) D2 (Standard4) D3 (Standard5) D4 (Standard5) D5 (Standard6) D6 (Standard6) F1 (Standard7) F2 (Standard7) F3 (Standard8) F4 (Standard8) F5 (Standard9) F6 (Standard9) G1 (Pos Control1) G2 (Pos Control1) H1 (Neg Control1) H2 (Neg Control1) DetailsPlate 1 CalculationsExpression = x>1.2BA1 (D133 v 757973E) x = 6.36372, Result = TRUE, Well AUTO-FLAGGEDBA2 (D133 v 757973E) x = 6.03536, Result = TRUE, Well AUTO-FLAGGEDBA3 (D398 v 757973E) x = 6.34632, Result = TRUE, Well AUTO-FLAGGEDBA4 (D398 v 757973E) x = 6.84503, Result = TRUE, Well AUTO-FLAGGEDAA5 (D77 v 757973E) x = 6.94115, Result = TRUE, Well AUTO-FLAGGEDAA6 (D77 v 757973E) x = 3.80451, Result = TRUE, Well AUTO-FLAGGEDAC1 (D113 v 720281J) x = 1.4772, Result = TRUE, Well AUTO-FLAGGEDBC2 (D113 v 720281J) x = 3.69847, Result = TRUE, Well AUTO-FLAGGEDBC3 (D398 v 720281J) x = 2.94512, Result = TRUE, Well AUTO-FLAGGEDBC4 (D398 v 720281J) x = 2.91467, Result = TRUE, Well AUTO-FLAGGEDAC5 (D77 v 720281J) x = 3.82577, Result = TRUE, Well AUTO-FLAGGEDAC6 (D77 v 720281J) x = 5.07989, Result = TRUE, Well AUTO-FLAGGEDBE1 (D113 v 7579696) x = 7.08287, Result = TRUE, Well AUTO-FLAGGEDBE2 (D113 v 7579696) x = 6.78915, Result = TRUE, Well AUTO-FLAGGEDBE3 (D398 v 7579696) x = 4.52679, Result = TRUE, Well AUTO-FLAGGEDBE4 (D398 v 7579696) x = 2.57389, Result = TRUE, Well AUTO-FLAGGEDAE5 (D77 v 7579696) x = 3.30053, Result = TRUE, Well AUTO-FLAGGEDAE6 (D77 v 7579696) x = 5.01172, Result = TRUE, Well AUTO-FLAGGEDTransform (4) Matrix ExpressionDetailsModule,C:\Program Files\Dazdaq\Manta\Transforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 888,918 bytes Created = 25 June 2004, 12:01:04 Calculations "w/w(0,1)"Input Matrices Integral () Output Matrices Control OINot Calculated&A1 (D133 v 757973E) A2 (D133 v 757973E) A3 (D398 v 757973E) A4 (D398 v 757973E) A5 (D77 v 757973E) A6 (D77 v 757973E) B1 (Standard1) B2 (Standard1) B3 (Standard2) B4 (Standard2) B5 (Standard3) B6 (Standard3) C1 (D113 v 720281J) C2 (D113 v 720281J) C3 (D398 v 720281J) C4 (D398 v 720281J) C5 (D77 v 720281J) C6 (D77 v 720281J) D1 (Standard4) D2 (Standard4) D3 (Standard5) D4 (Standard5) D5 (Standard6) D6 (Standard6) E1 (D113 v 7579696) E2 (D113 v 7579696) E3 (D398 v 7579696) E4 (D398 v 7579696) E5 (D77 v 7579696) E6 (D77 v 7579696) F1 (Standard7) F2 (Standard7) F3 (Standard8) F4 (Standard8) F5 (Standard9) F6 (Standard9) H1 (Neg Control1) H2 (Neg Control1) DetailsPlate 1 Calculations(G1 (Pos Control1) = w/w(0,1) = 4.93817(G2 (Pos Control1) = w/w(0,1) = 4.97723Group Calculations Control OID133 v 757973E)A1 = NOT INCLUDED - no result calculated)A2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D398 v 757973E)A3 = NOT INCLUDED - no result calculated)A4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. D77 v 757973E)A5 = NOT INCLUDED - no result calculated)A6 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D113 v 720281J)C1 = NOT INCLUDED - no result calculated)C2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D398 v 720281J)C3 = NOT INCLUDED - no result calculated)C4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. D77 v 720281J)C5 = NOT INCLUDED - no result calculated)C6 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D113 v 7579696)E1 = NOT INCLUDED - no result calculated)E2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D398 v 7579696)E3 = NOT INCLUDED - no result calculated)E4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. D77 v 7579696)E5 = NOT INCLUDED - no result calculated)E6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard1)B1 = NOT INCLUDED - no result calculated)B2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard2)B3 = NOT INCLUDED - no result calculated)B4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard3)B5 = NOT INCLUDED - no result calculated)B6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard4)D1 = NOT INCLUDED - no result calculated)D2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard5)D3 = NOT INCLUDED - no result calculated)D4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard6)D5 = NOT INCLUDED - no result calculated)D6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard7)F1 = NOT INCLUDED - no result calculated)F2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard8)F3 = NOT INCLUDED - no result calculated)F4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard9)F5 = NOT INCLUDED - no result calculated)F6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Pos Control1 G1 = 4.93817 G2 = 4.97723Average = 9.9154 / 2 = 4.95      !"#$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcdefghijklmnopqrstuvwxyz{|}~77 Neg Control1)H1 = NOT INCLUDED - no result calculated)H2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.#Transform (5) Validation ExpressionDetailsModule-C:\Program Files\Dazdaq\Manta\QTransforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 610,392 bytes Created = 25 June 2004, 12:01:40 CalculationsValidates: Pos Control1>2.0Input MatricesControl OI () Not CalculatedDetailsPlate 1 CalculationsPos Control1>2.0 - passedTransform (6) Auto Flag By WellDetailsModule,C:\Program Files\Dazdaq\Manta\Transforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 888,918 bytes Created = 25 June 2004, 12:01:04 Calculationsx >2.0Input MatricesControl OI () Not Calculated&A1 (D133 v 757973E) A2 (D133 v 757973E) A3 (D398 v 757973E) A4 (D398 v 757973E) A5 (D77 v 757973E) A6 (D77 v 757973E) B1 (Standard1) B2 (Standard1) B3 (Standard2) B4 (Standard2) B5 (Standard3) B6 (Standard3) C1 (D113 v 720281J) C2 (D113 v 720281J) C3 (D398 v 720281J) C4 (D398 v 720281J) C5 (D77 v 720281J) C6 (D77 v 720281J) D1 (Standard4) D2 (Standard4) D3 (Standard5) D4 (Standard5) D5 (Standard6) D6 (Standard6) E1 (D113 v 7579696) E2 (D113 v 7579696) E3 (D398 v 7579696) E4 (D398 v 7579696) E5 (D77 v 7579696) E6 (D77 v 7579696) F1 (Standard7) F2 (Standard7) F3 (Standard8) F4 (Standard8) F5 (Standard9) F6 (Standard9) H1 (Neg Control1) H2 (Neg Control1) DetailsPlate 1 CalculationsExpression = x >2.0@G1 (Pos Control1) x = 4.93817, Result = TRUE, Well AUTO-FLAGGED@G2 (Pos Control1) x = 4.97723, Result = TRUE, Well AUTO-FLAGGED2(13 October 2004 10:10:03) Calculations, version 9Raw Data Group CalculationsPlate 10nmAnalysisTransform (1) IntegralDetailsModule-C:\Program Files\Dazdaq\Manta\KRTransform.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 680,024 bytes Created = 25 June 2004, 12:01:14 CalculationseIntegrated area by trapezium method. Using all unflagged data points, Axes = (Cycle Number, Reading).Input Matrices 0nm (RLU) Output MatricesIntegralDetailsPlate 1 Calculations)NA1 (D133 v 757973E) Integral calculated by Trapezium method; Result = 232.565NA2 (D133 v 757973E) Integral calculated by Trapezium method; Result = 215.465NA3 (D398 v 757973E) Integral calculated by Trapezium method; Result = 262.646NA4 (D398 v 757973E) Integral calculated by Trapezium method; Result = 272.436MA5 (D77 v 757973E) Integral calculated by Trapezium method; Result = 287.541MA6 (D77 v 757973E) Integral calculated by Trapezium method; Result = 207.101IB1 (Standard1) Integral calculated by Trapezium method; Result = 36.5455IB2 (Standard1) Integral calculated by Trapezium method; Result = 35.7005IB3 (Standard2) Integral calculated by Trapezium method; Result = 41.3855IB4 (Standard2) Integral calculated by Trapezium method; Result = 39.8005IB5 (Standard3) Integral calculated by Trapezium method; Result = 41.4255IB6 (Standard3) Integral calculated by Trapezium method; Result = 54.4355NC1 (D113 v 720281J) Integral calculated by Trapezium method; Result = 239.041NC2 (D113 v 720281J) Integral calculated by Trapezium method; Result = 213.441NC3 (D398 v 720281J) Integral calculated by Trapezium method; Result = 201.816NC4 (D398 v 720281J) Integral calculated by Trapezium method; Result = 188.581LC5 (D77 v 720281J) Integral calculated by Trapezium method; Result = 265.72MC6 (D77 v 720281J) Integral calculated by Trapezium method; Result = 325.116ID1 (Standard4) Integral calculated by Trapezium method; Result = 161.821ID2 (Standard4) Integral calculated by Trapezium method; Result = 57.7105ID3 (Standard5) Integral calculated by Trapezium method; Result = 68.5255ID4 (Standard5) Integral calculated by Trapezium method; Result = 64.7005ID5 (Standard6) Integral calculated by Trapezium method; Result = 69.4555ID6 (Standard6) Integral calculated by Trapezium method; Result = 64.0005ME1 (D113 v 7579696) Integral calculated by Trapezium method; Result = 277.51NE2 (D113 v 7579696) Integral calculated by Trapezium method; Result = 330.465LE3 (D398 v 7579696) Integral calculated by Trapezium method; Result = 248.5NE4 (D398 v 7579696) Integral calculated by Trapezium method; Result = 135.645ME5 (D77 v 7579696) Integral calculated by Trapezium method; Result = 160.276LE6 (D77 v 7579696) Integral calculated by Trapezium method; Result = 249.31IF1 (Standard7) Integral calculated by Trapezium method; Result = 39.1805IF2 (Standard7) Integral calculated by Trapezium method; Result = 48.6755IF3 (Standard8) Integral calculated by Trapezium method; Result = 54.8955IF4 (Standard8) Integral calculated by Trapezium method; Result = 52.7005IF5 (Standard9) Integral calculated by Trapezium method; Result = 48.5605IF6 (Standard9) Integral calculated by Trapezium method; Result = 49.7455KG1 (Pos Control1) Integral calculated by Trapezium method; Result = 189.43LG2 (Pos Control1) Integral calculated by Trapezium method; Result = 221.241LH1 (Neg Control1) Integral calculated by Trapezium method; Result = 38.3605LH2 (Neg Control1) Integral calculated by Trapezium method; Result = 44.4505Group CalculationsIntegralD133 v 757973E A1 = 232.565 A2 = 215.465Average = 448.031 / 2 = 224.015D398 v 757973E A3 = 262.646 A4 = 272.436Average = 535.081 / 2 = 267.541 D77 v 757973E A5 = 287.541 A6 = 207.101Average = 494.641 / 2 = 247.321D113 v 720281J C1 = 239.041 C2 = 213.441Average = 452.481 / 2 = 226.241D398 v 720281J C3 = 201.816 C4 = 188.581Average = 390.396 / 2 = 195.198 D77 v 720281J C5 = 265.72 C6 = 325.116Average = 590.836 / 2 = 295.418D113 v 7579696 E1 = 277.51 E2 = 330.465Average = 607.976 / 2 = 303.988D398 v 7579696 E3 = 248.5 E4 = 135.645Average = 384.146 / 2 = 192.073 D77 v 7579696 E5 = 160.276 E6 = 249.31Average = 409.586 / 2 = 204.793 Standard1 B1 = 36.5455 B2 = 35.7005Average = 72.246 / 2 = 36.123 Standard2 B3 = 41.3855 B4 = 39.8005Average = 81.186 / 2 = 40.593 Standard3 B5 = 41.4255 B6 = 54.4355Average = 95.861 / 2 = 47.9305 Standard4 D1 = 161.821 D2 = 57.7105Average = 219.531 / 2 = 109.766 Standard5 D3 = 68.5255 D4 = 64.7005Average = 133.226 / 2 = 66.613 Standard6 D5 = 69.4555 D6 = 64.0005Average = 133.456 / 2 = 66.728 Standard7 F1 = 39.1805 F2 = 48.6755Average = 87.856 / 2 = 43.928 Standard8 F3 = 54.8955 F4 = 52.7005Average = 107.596 / 2 = 53.798 Standard9 F5 = 48.5605 F6 = 49.7455Average = 98.306 / 2 = 49.153 Pos Control1 G1 = 189.43 G2 = 221.241Average = 410.671 / 2 = 205.335 Neg Control1 H1 = 38.3605 H2 = 44.4505Average = 82.811 / 2 = 41.4055Transform (2) Matrix ExpressionDetailsModule,C:\Program Files\Dazdaq\Manta\Transforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 888,918 bytes Created = 25 June 2004, 12:01:04 Calculations "w/w(0,1)"Input Matrices Integral () Output Matrices Opsonic IndexNot CalculatedB1 (Standard1) B2 (Standard1) B3 (Standard2) B4 (Standard2) B5 (Standard3) B6 (Standard3) D1 (Standard4) D2 (Standard4) D3 (Standard5) D4 (Standard5) D5 (Standard6) D6 (Standard6) F1 (Standard7) F2 (Standard7) F3 (Standard8) F4 (Standard8) F5 (Standard9) F6 (Standard9) DetailsPlate 1 Calculations*A1 (D133 v 757973E) = w/w(0,1) = 6.36372*A2 (D133 v 757973E) = w/w(0,1) = 6.03536*A3 (D398 v 757973E) = w/w(0,1) = 6.34632*A4 (D398 v 757973E) = w/w(0,1) = 6.84503)A5 (D77 v 757973E) = w/w(0,1) = 6.94115)A6 (D77 v 757973E) = w/w(0,1) = 3.80451)C1 (D113 v 720281J) = w/w(0,1) = 1.4772*C2 (D113 v 720281J) = w/w(0,1) = 3.69847*C3 (D398 v 720281J) = w/w(0,1) = 2.94512*C4 (D398 v 720281J) = w/w(0,1) = 2.91467)C5 (D77 v 720281J) = w/w(0,1) = 3.82577)C6 (D77 v 720281J) = w/w(0,1) = 5.07989*E1 (D113 v 7579696) = w/w(0,1) = 7.08287*E2 (D113 v 7579696) = w/w(0,1) = 6.78915*E3 (D398 v 7579696) = w/w(0,1) = 4.52679*E4 (D398 v 7579696) = w/w(0,1) = 2.57389)E5 (D77 v 7579696) = w/w(0,1) = 3.30053)E6 (D77 v 7579696) = w/w(0,1) = 5.01172(G1 (Pos Control1) = w/w(0,1) = 4.93817(G2 (Pos Control1) = w/w(0,1) = 4.97723WH1 (Neg Control1) Could not evaluate: "w/w(0,1)" - Well offset out of microplate rangeWH2 (Neg Control1) Could not evaluate: "w/w(0,1)" - Well offset out of microplate rangeGroup Calculations Opsonic IndexD133 v 757973E A1 = 6.36372 A2 = 6.03536Average = 12.3991 / 2 = 6.19954D398 v 757973E A3 = 6.34632 A4 = 6.84503Average = 13.1913 / 2 = 6.59567 D77 v 757973E A5 = 6.94115 A6 = 3.80451Average = 10.7457 / 2 = 5.37283D113 v 720281J C1 = 1.4772 C2 = 3.69847Average = 5.17566 / 2 = 2.58783D398 v 720281J C3 = 2.94512 C4 = 2.91467Average = 5.85978 / 2 = 2.92989 D77 v 720281J C5 = 3.82577 C6 = 5.07989Average = 8.90566 / 2 = 4.45283D113 v 7579696 E1 = 7.08287 E2 = 6.78915Average = 13.872 / 2 = 6.93601D398 v 7579696 E3 = 4.52679 E4 = 2.57389Average = 7.10069 / 2 = 3.55034 D77 v 7579696 E5 = 3.30053 E6 = 5.01172Average = 8.31225 / 2 = 4.15613 Standard1)B1 = NOT INCLUDED - no result calculated)B2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard2)B3 = NOT INCLUDED - no result calculated)B4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard3)B5 = NOT INCLUDED - no result calculated)B6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard4)D1 = NOT INCLUDED - no result calculated)D2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard5)D3 = NOT INCLUDED - no result calculated)D4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard6)D5 = NOT INCLUDED - no result calculated)D6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard7)F1 = NOT INCLUDED - no result calculated)F2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard8)F3 = NOT INCLUDED - no result calculated)F4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard9)F5 = NOT INCLUDED - no result calculated)F6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Pos Control1 G1 = 4.93817 G2 = 4.97723Average = 9.9154 / 2 = 4.9577 Neg Control1&H1 = NOT INCLUDED - calculation error&H2 = NOT INCLUDED - calculation error&All wells in this group were excluded.Transform (3) Auto Flag By WellDetailsModule,C:\Program Files\Dazdaq\Manta\Transforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 888,918 bytes Created = 25 June 2004, 12:01:04 Calculationsx>1.2Input MatricesOpsonic Index () Not CalculatedB1 (Standard1) B2 (Standard1) B3 (Standard2) B4 (Standard2) B5 (Standard3) B6 (Standard3) D1 (Standard4) D2 (Standard4) D3 (Standard5) D4 (Standard5) D5 (Standard6) D6 (Standard6) F1 (Standard7) F2 (Standard7) F3 (Standard8) F4 (Standard8) F5 (Standard9) F6 (Standard9) G1 (Pos Control1) G2 (Pos Control1) H1 (Neg Control1) H2 (Neg Control1) DetailsPlate 1 CalculationsExpression = x>1.2BA1 (D133 v 757973E) x = 6.36372, Result = TRUE, Well AUTO-FLAGGEDBA2 (D133 v 757973E) x = 6.03536, Result = TRUE, Well AUTO-FLAGGEDBA3 (D398 v 757973E) x = 6.34632, Result = TRUE, Well AUTO-FLAGGEDBA4 (D398 v 757973E) x = 6.84503, Result = TRUE, Well AUTO-FLAGGEDAA5 (D77 v 757973E) x = 6.94115, Result = TRUE, Well AUTO-FLAGGEDAA6 (D77 v 757973E) x = 3.80451, Result = TRUE, Well AUTO-FLAGGEDAC1 (D113 v 720281J) x = 1.4772, Result = TRUE, Well AUTO-FLAGGEDBC2 (D113 v 720281J) x = 3.69847, Result = TRUE, Well AUTO-FLAGGEDBC3 (D398 v 720281J) x = 2.94512, Result = TRUE, Well AUTO-FLAGGEDBC4 (D398 v 720281J) x = 2.91467, Result = TRUE, Well AUTO-FLAGGEDAC5 (D77 v 720281J) x = 3.82577, Result = TRUE, Well AUTO-FLAGGEDAC6 (D77 v 720281J) x = 5.07989, Result = TRUE, Well AUTO-FLAGGEDBE1 (D113 v 7579696) x = 7.08287, Result = TRUE, Well AUTO-FLAGGEDBE2 (D113 v 7579696) x = 6.78915, Result = TRUE, Well AUTO-FLAGGEDBE3 (D398 v 7579696) x = 4.52679, Result = TRUE, Well AUTO-FLAGGEDBE4 (D398 v 7579696) x = 2.57389, Result = TRUE, Well AUTO-FLAGGEDAE5 (D77 v 7579696) x = 3.30053, Result = TRUE, Well AUTO-FLAGGEDAE6 (D77 v 7579696) x = 5.01172, Result = TRUE, Well AUTO-FLAGGEDTransform (4) Matrix ExpressionDetailsModule,C:\Program Files\Dazdaq\Manta\Transforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 888,918 bytes Created = 25 June 2004, 12:01:04 Calculations "w/w(0,1)"Input Matrices Integral () Output Matrices Control OINot Calculated&A1 (D133 v 757973E) A2 (D133 v 757973E) A3 (D398 v 757973E) A4 (D398 v 757973E) A5 (D77 v 757973E) A6 (D77 v 757973E) B1 (Standard1) B2 (Standard1) B3 (Standard2) B4 (Standard2) B5 (Standard3) B6 (Standard3) C1 (D113 v 720281J) C2 (D113 v 720281J) C3 (D398 v 720281J) C4 (D398 v 720281J) C5 (D77 v 720281J) C6 (D77 v 720281J) D1 (Standard4) D2 (Standard4) D3 (Standard5) D4 (Standard5) D5 (Standard6) D6 (Standard6) E1 (D113 v 7579696) E2 (D113 v 7579696) E3 (D398 v 7579696) E4 (D398 v 7579696) E5 (D77 v 7579696) E6 (D77 v 7579696) F1 (Standard7) F2 (Standard7) F3 (Standard8) F4 (Standard8) F5 (Standard9) F6 (Standard9) H1 (Neg Control1) H2 (Neg Control1) DetailsPlate 1 Calculations(G1 (Pos Control1) = w/w(0,1) = 4.93817(G2 (Pos Control1) = w/w(0,1) = 4.97723Group Calculations Control OID133 v 757973E)A1 = NOT INCLUDED - no result calculated)A2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D398 v 757973E)A3 = NOT INCLUDED - no result calculated)A4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. D77 v 757973E)A5 = NOT INCLUDED - no result calculated)A6 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D113 v 720281J)C1 = NOT INCLUDED - no result calculated)C2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D398 v 720281J)C3 = NOT INCLUDED - no result calculated)C4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. D77 v 720281J)C5 = NOT INCLUDED - no result calculated)C6 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D113 v 7579696)E1 = NOT INCLUDED - no result calculated)E2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D398 v 7579696)E3 = NOT INCLUDED - no result calculated)E4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. D77 v 7579696)E5 = NOT INCLUDED - no result calculated)E6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard1)B1 = NOT INCLUDED - no result calculated)B2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard2)B3 = NOT INCLUDED - no result calculated)B4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard3)B5 = NOT INCLUDED - no result calculated)B6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard4)D1 = NOT INCLUDED - no result calculated)D2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard5)D3 = NOT INCLUDED - no result calculated)D4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard6)D5 = NOT INCLUDED - no result calculated)D6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard7)F1 = NOT INCLUDED - no result calculated)F2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard8)F3 = NOT INCLUDED - no result calculated)F4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard9)F5 = NOT INCLUDED - no result calculated)F6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Pos Control1 G1 = 4.93817 G2 = 4.97723Average = 9.9154 / 2 = 4.9577 Neg Control1)H1 = NOT INCLUDED - no result calculated)H2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.#Transform (5) Validation ExpressionDetailsModule-C:\Program Files\Dazdaq\Manta\QTransforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 610,392 bytes Created = 25 June 2004, 12:01:40 CalculationsValidates: Pos Control1>2.0Input MatricesControl OI () Not CalculatedDetailsPlate 1 CalculationsPos Control1>2.0 - passed(13 October 2004 10:11:04) Saved As C:\Documents and Settings\LyP\Personal\My Assays\Results\Slow Kinetics\Slow Kinetics (1).arsy(13 October 2004 10:39:01) Accessing E:\LAB_SERV\PDS\IMM\Manta Data\My Assays\Results\Slow Kinetics\Slow Kinetics (1).arsy(13 October 2004 11:43:15) Accessing E:\LAB_SERV\PDS\IMM\Manta Data\My Assays\Results\Slow Kinetics\Slow Kinetics (1).ars3(13 October 2004 11:55:44) Calculations, version 10Raw Data Group CalculationsPlate 10nmAnalysisTransform (1) IntegralDetailsModule-C:\Program Files\Dazdaq\Manta\KRTransform.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 680,024 bytes Created = 25 June 2004, 12:01:14 CalculationsIntegrated area by trapezium method. Using all unflagged points in between and including X = "1.00756" and X = "100", Axes = (Cycle Number, Reading).Input Matrices 0nm (RLU) Output MatricesIntegralDetailsPlate 1 Calculations)sA1 (D133 v 757973E) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 232.412sA2 (D133 v 757973E) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 215.362sA3 (D398 v 757973E) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 262.507sA4 (D398 v 757973E) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 272.272rA5 (D77 v 757973E) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 287.357rA6 (D77 v 757973E) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 206.972mB1 (Standard1) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 36.467mB2 (Standard1) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 35.542mB3 (Standard2) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 41.187mB4 (Standard2) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 39.637mB5 (Standard3) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 41.282mB6 (Standard3) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 54.277sC1 (D113 v 720281J) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 238.872sC2 (D113 v 720281J) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 213.342sC3 (D398 v 720281J) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 201.677sC4 (D398 v 720281J) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 188.397rC5 (D77 v 720281J) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 265.382rC6 (D77 v 720281J) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 324.902nD1 (Standard4) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 160.972mD2 (Standard4) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 57.517mD3 (Standard5) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 68.262mD4 (Standard5) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 64.497mD5 (Standard6) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 69.257mD6 (Standard6) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 63.767sE1 (D113 v 7579696) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 277.262sE2 (D113 v 7579696) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 330.237sE3 (D398 v 7579696) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 247.667sE4 (D398 v 7579696) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 135.447rE5 (D77 v 7579696) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 160.117rE6 (D77 v 7579696) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 249.147mF1 (Standard7) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 39.032mF2 (Standard7) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 48.562mF3 (Standard8) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 54.712mF4 (Standard8) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 52.592mF5 (Standard9) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 48.362mF6 (Standard9) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 49.532qG1 (Pos Control1) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 189.232qG2 (Pos Control1) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 221.087pH1 (Neg Control1) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 38.182pH2 (Neg Control1) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 44.197Group CalculationsIntegralD133 v 757973E A1 = 232.412 A2 = 215.362Average = 447.774 / 2 = 223.887D398 v 757973E A3 = 262.507 A4 = 272.272Average = 534.779 / 2 = 267.39 D77 v 757973E A5 = 287.357 A6 = 206.972Average = 494.329 / 2 = 247.164D113 v 720281J C1 = 238.872 C2 = 213.342Average = 452.214 / 2 = 226.107D398 v 720281J C3 = 201.677 C4 = 188.397Average = 390.074 / 2 = 195.037 D77 v 720281J C5 = 265.382 C6 = 324.902Average = 590.284 / 2 = 295.142D113 v 7579696 E1 = 277.262 E2 = 330.237Average = 607.499 / 2 = 303.75D398 v 7579696 E3 = 247.667 E4 = 135.447Average = 383.114 / 2 = 191.557 D77 v 7579696 E5 = 160.117 E6 = 249.147Average = 409.264 / 2 = 204.632 Standard1 B1 = 36.467 B2 = 35.542Average = 72.009 / 2 = 36.0045 Standard2 B3 = 41.187 B4 = 39.637Average = 80.824 / 2 = 40.412 Standard3 B5 = 41.282 B6 = 54.277Average = 95.559 / 2 = 47.7795 Standard4 D1 = 160.972 D2 = 57.517Average = 218.489 / 2 = 109.245 Standard5 D3 = 68.262 D4 = 64.497Average = 132.759 / 2 = 66.3795 Standard6 D5 = 69.257 D6 = 63.767Average = 133.024 / 2 = 66.512 Standard7 F1 = 39.032 F2 = 48.562Average = 87.594 / 2 = 43.797 Standard8 F3 = 54.712 F4 = 52.592Average = 107.304 / 2 = 53.652 Standard9 F5 = 48.362 F6 = 49.532Average = 97.894 / 2 = 48.947 Pos Control1 G1 = 189.232 G2 = 221.087Average = 410.319 / 2 = 205.159 Neg Control1 H1 = 38.182 H2 = 44.197Average = 82.379 / 2 = 41.1895Transform (2) Matrix ExpressionDetailsModule,C:\Program Files\Dazdaq\Manta\Transforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 888,918 bytes Created = 25 June 2004, 12:01:04 Calculations "w/w(0,1)"Input Matrices Integral () Output Matrices Opsonic IndexNot CalculatedB1 (Standard1) B2 (Standard1) B3 (Standard2) B4 (Standard2) B5 (Standard3) B6 (Standard3) D1 (Standard4) D2 (Standard4) D3 (Standard5) D4 (Standard5) D5 (Standard6) D6 (Standard6) F1 (Standard7) F2 (Standard7) F3 (Standard8) F4 (Standard8) F5 (Standard9) F6 (Standard9) DetailsPlate 1 Calculations*A1 (D133 v 757973E) = w/w(0,1) = 6.37321*A2 (D133 v 757973E) = w/w(0,1) = 6.05937*A3 (D398 v 757973E) = w/w(0,1) = 6.37354*A4 (D398 v 757973E) = w/w(0,1) = 6.86914)A5 (D77 v 757973E) = w/w(0,1) = 6.96083)A6 (D77 v 757973E) = w/w(0,1) = 3.81325*C1 (D113 v 720281J) = w/w(0,1) = 1.48394)C2 (D113 v 720281J) = w/w(0,1) = 3.7092*C3 (D398 v 720281J) = w/w(0,1) = 2.95445*C4 (D398 v 720281J) = w/w(0,1) = 2.92102)C5 (D77 v 720281J) = w/w(0,1) = 3.83184)C6 (D77 v 720281J) = w/w(0,1) = 5.09514*E1 (D113 v 7579696) = w/w(0,1) = 7.10345*E2 (D113 v 7579696) = w/w(0,1) = 6.80032*E3 (D398 v 7579696) = w/w(0,1) = 4.52674*E4 (D398 v 7579696) = w/w(0,1) = 2.57543(E5 (D77 v 7579696) = w/w(0,1) = 3.3108)E6 (D77 v 7579696) = w/w(0,1) = 5.03002(G1 (Pos Control1) = w/w(0,1) = 4.95605(G2 (Pos Control1) = w/w(0,1) = 5.00231WH1 (Neg Control1) Could not evaluate: "w/w(0,1)" - Well offset out of microplate rangeWH2 (Neg Control1) Could not evaluate: "w/w(0,1)" - Well offset out of microplate rangeGroup Calculations Opsonic IndexD133 v 757973E A1 = 6.37321 A2 = 6.05937Average = 12.4326 / 2 = 6.21629D398 v 757973E A3 = 6.37354 A4 = 6.86914Average = 13.2427 / 2 = 6.62134 D77 v 757973E A5 = 6.96083 A6 = 3.81325Average = 10.7741 / 2 = 5.38704D113 v 720281J C1 = 1.48394 C2 = 3.7092Average = 5.19313 / 2 = 2.59657D398 v 720281J C3 = 2.95445 C4 = 2.92102Average = 5.87547 / 2 = 2.93774 D77 v 720281J C5 = 3.83184 C6 = 5.09514Average = 8.92699 / 2 = 4.46349D113 v 7579696 E1 = 7.10345 E2 = 6.80032Average = 13.9038 / 2 = 6.95189D398 v 7579696 E3 = 4.52674 E4 = 2.57543Average = 7.10217 / 2 = 3.55108 D77 v 7579696 E5 = 3.3108 E6 = 5.03002Average = 8.34082 / 2 = 4.17041 Standard1)B1 = NOT INCLUDED - no result calculated)B2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard2)B3 = NOT INCLUDED - no result calculated)B4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard3)B5 = NOT INCLUDED - no result calculated)B6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard4)D1 = NOT INCLUDED - no result calculated)D2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard5)D3 = NOT INCLUDED - no result calculated)D4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard6)D5 = NOT INCLUDED - no result calculated)D6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard7)F1 = NOT INCLUDED - no result calculated)F2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard8)F3 = NOT INCLUDED - no result calculated)F4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard9)F5 = NOT INCLUDED - no result calculated)F6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Pos Control1 G1 = 4.95605 G2 = 5.00231Average = 9.95836 / 2 = 4.97918 Neg Control1&H1 = NOT INCLUDED - calculation error&H2 = NOT INCLUDED - calculation error&All wells in this group were excluded.Transform (3) Auto Flag By WellDetailsModule,C:\Program Files\Dazdaq\Manta\Transforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 888,918 bytes Created = 25 June 2004, 12:01:04 Calculationsx>1.2Input MatricesOpsonic Index () Not CalculatedB1 (Standard1) B2 (Standard1) B3 (Standard2) B4 (Standard2) B5 (Standard3) B6 (Standard3) D1 (Standard4) D2 (Standard4) D3 (Standard5) D4 (Standard5) D5 (Standard6) D6 (Standard6) F1 (Standard7) F2 (Standard7) F3 (Standard8) F4 (Standard8) F5 (Standard9) F6 (Standard9) G1 (Pos Control1) G2 (Pos Control1) H1 (Neg Control1) H2 (Neg Control1) DetailsPlate 1 CalculationsExpression = x>1.2BA1 (D133 v 757973E) x = 6.37321, Result = TRUE, Well AUTO-FLAGGEDBA2 (D133 v 757973E) x = 6.05937, Result = TRUE, Well AUTO-FLAGGEDBA3 (D398 v 757973E) x = 6.37354, Result = TRUE, Well AUTO-FLAGGEDBA4 (D398 v 757973E) x = 6.86914, Result = TRUE, Well AUTO-FLAGGEDAA5 (D77 v 757973E) x = 6.96083, Result = TRUE, Well AUTO-FLAGGEDAA6 (D77 v 757973E) x = 3.81325, Result = TRUE, Well AUTO-FLAGGEDBC1 (D113 v 720281J) x = 1.48394, Result = TRUE, Well AUTO-FLAGGEDAC2 (D113 v 720281J) x = 3.7092, Result = TRUE, Well AUTO-FLAGGEDBC3 (D398 v 720281J) x = 2.95445, Result = TRUE, Well AUTO-FLAGGEDBC4 (D398 v 720281J) x = 2.92102, Result = TRUE, Well AUTO-FLAGGEDAC5 (D77 v 720281J) x = 3.83184, Result = TRUE, Well AUTO-FLAGGEDAC6 (D77 v 720281J) x = 5.09514, Result = TRUE, Well AUTO-FLAGGEDBE1 (D113 v 7579696) x = 7.10345, Result = TRUE, Well AUTO-FLAGGEDBE2 (D113 v 7579696) x = 6.80032, Result = TRUE, Well AUTO-FLAGGEDBE3 (D398 v 7579696) x = 4.52674, Result = TRUE, Well AUTO-FLAGGEDBE4 (D398 v 7579696) x = 2.57543, Result = TRUE, Well AUTO-FLAGGED@E5 (D77 v 7579696) x = 3.3108, Result = TRUE, Well AUTO-FLAGGEDAE6 (D77 v 7579696) x = 5.03002, Result = TRUE, Well AUTO-FLAGGEDTransform (4) Matrix ExpressionDetailsModule,C:\Program Files\Dazdaq\Manta\Transforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 888,918 bytes Created = 25 June 2004, 12:01:04 Calculations "w/w(0,1)"Input Matrices Integral () Output Matrices Control OINot Calculated&A1 (D133 v 757973E) A2 (D133 v 757973E) A3 (D398 v 757973E) A4 (D398 v 757973E) A5 (D77 v 757973E) A6 (D77 v 757973E) B1 (Standard1) B2 (Standard1) B3 (Standard2) B4 (Standard2) B5 (Standard3) B6 (Standard3) C1 (D113 v 720281J) C2 (D113 v 720281J) C3 (D398 v 720281J) C4 (D398 v 720281J) C5 (D77 v 720281J) C6 (D77 v 720281J) D1 (Standard4) D2 (Standard4) D3 (Standard5) D4 (Standard5) D5 (Standard6) D6 (Standard6) E1 (D113 v 7579696) E2 (D113 v 7579696) E3 (D398 v 7579696) E4 (D398 v 7579696) E5 (D77 v 7579696) E6 (D77 v 7579696) F1 (Standard7) F2 (Standard7) F3 (Standard8) F4 (Standard8) F5 (Standard9) F6 (Standard9) H1 (Neg Control1) H2 (Neg Control1) DetailsPlate 1 Calculations(G1 (Pos Control1) = w/w(0,1) = 4.95605(G2 (Pos Control1) = w/w(0,1) = 5.00231Group Calculations Control OID133 v 757973E)A1 = NOT INCLUDED - no result calculated)A2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D398 v 757973E)A3 = NOT INCLUDED - no result calculated)A4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. D77 v 757973E)A5 = NOT INCLUDED - no result calculated)A6 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D113 v 720281J)C1 = NOT INCLUDED - no result calculated)C2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D398 v 720281J)C3 = NOT INCLUDED - no result calculated)C4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. D77 v 720281J)C5 = NOT INCLUDED - no result calculated)C6 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D113 v 7579696)E1 = NOT INCLUDED - no result calculated)E2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D398 v 7579696)E3 = NOT INCLUDED - no result calculated)E4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. D77 v 7579696)E5 = NOT INCLUDED - no result calculated)E6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard1)B1 = NOT INCLUDED - no result calculated)B2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard2)B3 = NOT INCLUDED - no result calculated)B4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard3)B5 = NOT INCLUDED - no result calculated)B6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard4)D1 = NOT INCLUDED - no result calculated)D2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard5)D3 = NOT INCLUDED - no result calculated)D4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard6)D5 = NOT INCLUDED - no result calculated)D6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard7)F1 = NOT INCLUDED - no result calculated)F2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard8)F3 = NOT INCLUDED - no result calculated)F4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard9)F5 = NOT INCLUDED - no result calculated)F6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Pos Control1 G1 = 4.95605 G2 = 5.00231Average = 9.95836 / 2 = 4.97918 Neg Control1)H1 = NOT INCLUDED - no result calculated)H2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.#Transform (5) Validation ExpressionDetailsModule-C:\Program Files\Dazdaq\Manta\QTransforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 610,392 bytes Created = 25 June 2004, 12:01:40 CalculationsValidates: Pos Control1>2.0Input MatricesControl OI () Not CalculatedDetailsPlate 1 CalculationsPos Control1>2.0 - passedy(13 October 2004 12:47:10) Accessing E:\LAB_SERV\PDS\IMM\Manta Data\My Assays\Results\Slow Kinetics\Slow Kinetics (1).ars3(13 October 2004 12:51:09) Calculations, version 11Raw Data Group CalculationsPlate 10nmAnalysisTransform (1) IntegralDetailsModule-C:\Program Files\Dazdaq\Manta\KRTransform.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 680,024 bytes Created = 25 June 2004, 12:01:14 CalculationsIntegrated area by trapezium method. Using all unflagged points in between and including X = "1.00756" and X = "100", Axes = (Cycle Number, Reading).Input Matrices 0nm (RLU) Output MatricesIntegralDetailsPlate 1 Calculations)sA1 (D133 v 757973E) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 232.412sA2 (D133 v 757973E) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 215.362sA3 (D398 v 757973E) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 262.507sA4 (D398 v 757973E) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 272.272rA5 (D77 v 757973E) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 287.357rA6 (D77 v 757973E) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 206.972mB1 (Standard1) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 36.467mB2 (Standard1) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 35.542mB3 (Standard2) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 41.187mB4 (Standard2) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 39.637mB5 (Standard3) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 41.282mB6 (Standard3) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 54.277sC1 (D113 v 720281J) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 238.872sC2 (D113 v 720281J) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 213.342sC3 (D398 v 720281J) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 201.677sC4 (D398 v 720281J) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 188.397rC5 (D77 v 720281J) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 265.382rC6 (D77 v 720281J) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 324.902nD1 (Standard4) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 160.972mD2 (Standard4) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 57.517mD3 (Standard5) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 68.262mD4 (Standard5) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 64.497mD5 (Standard6) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 69.257mD6 (Standard6) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 63.767sE1 (D113 v 7579696) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 277.262sE2 (D113 v 7579696) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 330.237sE3 (D398 v 7579696) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 247.667sE4 (D398 v 7579696) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 135.447rE5 (D77 v 7579696) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 160.117rE6 (D77 v 7579696) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 249.147mF1 (Standard7) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 39.032mF2 (Standard7) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 48.562mF3 (Standard8) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 54.712mF4 (Standard8) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 52.592mF5 (Standard9) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 48.362mF6 (Standard9) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 49.532qG1 (Pos Control1) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 189.232qG2 (Pos Control1) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 221.087pH1 (Neg Control1) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 38.182pH2 (Neg Control1) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 44.197Group CalculationsIntegralD133 v 757973E A1 = 232.412 A2 = 215.362Average = 447.774 / 2 = 223.887D398 v 757973E A3 = 262.507 A4 = 272.272Average = 534.779 / 2 = 267.39 D77 v 757973E A5 = 287.357 A6 = 206.972Average = 494.329 / 2 = 247.164D113 v 720281J C1 = 238.872 C2 = 213.342Average = 452.214 / 2 = 226.107D398 v 720281J C3 = 201.677 C4 = 188.397Average = 390.074 / 2 = 195.037 D77 v 720281J C5 = 265.382 C6 = 324.902Average = 590.284 / 2 = 295.142D113 v 7579696 E1 = 277.262 E2 = 330.237Average = 607.499 / 2 = 303.75D398 v 7579696 E3 = 247.667 E4 = 135.447Average = 383.114 / 2 = 191.557 D77 v 7579696 E5 = 160.117 E6 = 249.147Average = 409.264 / 2 = 204.632 Standard1 B1 = 36.467 B2 = 35.542Average = 72.009 / 2 = 36.0045 Standard2 B3 = 41.187 B4 = 39.637Average = 80.824 / 2 = 40.412 Standard3 B5 = 41.282 B6 = 54.277Average = 95.559 / 2 = 47.7795 Standard4 D1 = 160.972 D2 = 57.517Average = 218.489 / 2 = 109.245 Standard5 D3 = 68.262 D4 = 64.497Average = 132.759 / 2 = 66.3795 Standard6 D5 = 69.257 D6 = 63.767Average = 133.024 / 2 = 66.512 Standard7 F1 = 39.032 F2 = 48.562Average = 87.594 / 2 = 43.797 Standard8 F3 = 54.712 F4 = 52.592Average = 107.304 / 2 = 53.652 Standard9 F5 = 48.362 F6 = 49.532Average = 97.894 / 2 = 48.947 Pos Control1 G1 = 189.232 G2 = 221.087Average = 410.319 / 2 = 205.159 Neg Control1 H1 = 38.182 H2 = 44.197Average = 82.379 / 2 = 41.1895Transform (2) Matrix ExpressionDetailsModule,C:\Program Files\Dazdaq\Manta\Transforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 888,918 bytes Created = 25 June 2004, 12:01:04 Calculations "w/w(0,1)"Input Matrices Integral () Output Matrices Opsonic IndexNot CalculatedB1 (Standard1) B2 (Standard1) B3 (Standard2) B4 (Standard2) B5 (Standard3) B6 (Standard3) D1 (Standard4) D2 (Standard4) D3 (Standard5) D4 (Standard5) D5 (Standard6) D6 (Standard6) F1 (Standard7) F2 (Standard7) F3 (Standard8) F4 (Standard8) F5 (Standard9) F6 (Standard9) H1 (Neg Control1) H2 (Neg Control1) DetailsPlate 1 Calculations*A1 (D133 v 757973E) = w/w(0,1) = 6.37321*A2 (D133 v 757973E) = w/w(0,1) = 6.05937*A3 (D398 v 757973E) = w/w(0,1) = 6.37354*A4 (D398 v 757973E) = w/w(0,1) = 6.86914)A5 (D77 v 757973E) = w/w(0,1) = 6.96083)A6 (D77 v 757973E) = w/w(0,1) = 3.81325*C1 (D113 v 720281J) = w/w(0,1) = 1.48394)C2 (D113 v 720281J) = w/w(0,1) = 3.7092*C3 (D398 v 720281J) = w/w(0,1) = 2.95445*C4 (D398 v 720281J) = w/w(0,1) = 2.92102)C5 (D77 v 720281J) = w/w(0,1) = 3.83184)C6 (D77 v 720281J) = w/w(0,1) = 5.09514*E1 (D113 v 7579696) = w/w(0,1) = 7.10345*E2 (D113 v 7579696) = w/w(0,1) = 6.80032*E3 (D398 v 7579696) = w/w(0,1) = 4.52674*E4 (D398 v 7579696) = w/w(0,1) = 2.57543(E5 (D77 v 7579696) = w/w(0,1) = 3.3108)E6 (D77 v 7579696) = w/w(0,1) = 5.03002(G1 (Pos Control1) = w/w(0,1) = 4.95605(G2 (Pos Control1) = w/w(0,1) = 5.00231Group Calculations Opsonic IndexD133 v 757973E A1 = 6.37321 A2 = 6.05937Average = 12.4326 / 2 = 6.21629D398 v 757973E A3 = 6.37354 A4 = 6.86914Average = 13.2427 / 2 = 6.62134 D77 v 757973E A5 = 6.96083 A6 = 3.81325Average = 10.7741 / 2 = 5.38704D113 v 720281J C1 = 1.48394 C2 = 3.7092Average = 5.19313 / 2 = 2.59657D398 v 720281J C3 = 2.95445 C4 = 2.92102Average = 5.87547 / 2 = 2.93774 D77 v 720281J C5 = 3.83184 C6 = 5.09514Average = 8.92699 / 2 = 4.46349D113 v 7579696 E1 = 7.10345 E2 = 6.80032Average = 13.9038 / 2 = 6.95189D398 v 7579696 E3 = 4.52674 E4 = 2.57543Average = 7.10217 / 2 = 3.55108 D77 v 7579696 E5 = 3.3108 E6 = 5.03002Average = 8.34082 / 2 = 4.17041 Standard1)B1 = NOT INCLUDED - no result calculated)B2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard2)B3 = NOT INCLUDED - no result calculated)B4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard3)B5 = NOT INCLUDED - no result calculated)B6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard4)D1 = NOT INCLUDED - no result calculated)D2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard5)D3 = NOT INCLUDED - no result calculated)D4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard6)D5 = NOT INCLUDED - no result calculated)D6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard7)F1 = NOT INCLUDED - no result calculated)F2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard8)F3 = NOT INCLUDED - no result calculated)F4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard9)F5 = NOT INCLUDED - no result calculated)F6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Pos Control1 G1 = 4.95605 G2 = 5.00231Average = 9.95836 / 2 = 4.97918 Neg Control1)H1 = NOT INCLUDED - no result calculated)H2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.Transform (3) Auto Flag By WellDetailsModule,C:\Program Files\Dazdaq\Manta\Transforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 888,918 bytes Created = 25 June 2004, 12:01:04 Calculationsx>1.2Input MatricesOpsonic Index () Not CalculatedB1 (Standard1) B2 (Standard1) B3 (Standard2) B4 (Standard2) B5 (Standard3) B6 (Standard3) D1 (Standard4) D2 (Standard4) D3 (Standard5) D4 (Standard5) D5 (Standard6) D6 (Standard6) F1 (Standard7) F2 (Standard7) F3 (Standard8) F4 (Standard8) F5 (Standard9) F6 (Standard9) G1 (Pos Control1) G2 (Pos Control1) H1 (Neg Control1) H2 (Neg Control1) DetailsPlate 1 CalculationsExpression = x>1.2BA1 (D133 v 757973E) x = 6.37321, Result = TRUE, Well AUTO-FLAGGEDBA2 (D133 v 757973E) x = 6.05937, Result = TRUE, Well AUTO-FLAGGEDBA3 (D398 v 757973E) x = 6.37354, Result = TRUE, Well AUTO-FLAGGEDBA4 (D398 v 757973E) x = 6.86914, Result = TRUE, Well AUTO-FLAGGEDAA5 (D77 v 757973E) x = 6.96083, Result = TRUE, Well AUTO-FLAGGEDAA6 (D77 v 757973E) x = 3.81325, Result = TRUE, Well AUTO-FLAGGEDBC1 (D113 v 720281J) x = 1.48394, Result = TRUE, Well AUTO-FLAGGEDAC2 (D113 v 720281J) x = 3.7092, Result = TRUE, Well AUTO-FLAGGEDBC3 (D398 v 720281J) x = 2.95445, Result = TRUE, Well AUTO-FLAGGEDBC4 (D398 v 720281J) x = 2.92102, Result = TRUE, Well AUTO-FLAGGEDAC5 (D77 v 720281J) x = 3.83184, Result = TRUE, Well AUTO-FLAGGEDAC6 (D77 v 720281J) x = 5.09514, Result = TRUE, Well AUTO-FLAGGEDBE1 (D113 v 7579696) x = 7.10345, Result = TRUE, Well AUTO-FLAGGEDBE2 (D113 v 7579696) x = 6.80032, Result = TRUE, Well AUTO-FLAGGEDBE3 (D398 v 7579696) x = 4.52674, Result = TRUE, Well AUTO-FLAGGEDBE4 (D398 v 7579696) x = 2.57543, Result = TRUE, Well AUTO-FLAGGED@E5 (D77 v 7579696) x = 3.3108, Result = TRUE, Well AUTO-FLAGGEDAE6 (D77 v 7579696) x = 5.03002, Result = TRUE, Well AUTO-FLAGGEDTransform (4) Matrix ExpressionDetailsModule,C:\Program Files\Dazdaq\Manta\Transforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 888,918 bytes Created = 25 June 2004, 12:01:04 Calculations "w/w(0,1)"Input Matrices Integral () Output Matrices Control OINot Calculated&A1 (D133 v 757973E) A2 (D133 v 757973E) A3 (D398 v 757973E) A4 (D398 v 757973E) A5 (D77 v 757973E) A6 (D77 v 757973E) B1 (Standard1) B2 (Standard1) B3 (Standard2) B4 (Standard2) B5 (Standard3) B6 (Standard3) C1 (D113 v 720281J) C2 (D113 v 720281J) C3 (D398 v 720281J) C4 (D398 v 720281J) C5 (D77 v 720281J) C6 (D77 v 720281J) D1 (Standard4) D2 (Standard4) D3 (Standard5) D4 (Standard5) D5 (Standard6) D6 (Standard6) E1 (D113 v 7579696) E2 (D113 v 7579696) E3 (D398 v 7579696) E4 (D398 v 7579696) E5 (D77 v 7579696) E6 (D77 v 7579696) F1 (Standard7) F2 (Standard7) F3 (Standard8) F4 (Standard8) F5 (Standard9) F6 (Standard9) H1 (Neg Control1) H2 (Neg Control1) DetailsPlate 1 Calculations(G1 (Pos Control1) = w/w(0,1) = 4.95605(G2 (Pos Control1) = w/w(0,1) = 5.00231Group Calculations Control OID133 v 757973E)A1 = NOT INCLUDED - no result calculated)A2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D398 v 757973E)A3 = NOT INCLUDED - no result calculated)A4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. D77 v 757973E)A5 = NOT INCLUDED - no result calculated)A6 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D113 v 720281J)C1 = NOT INCLUDED - no result calculated)C2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D398 v 720281J)C3 = NOT INCLUDED - no result calculated)C4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. D77 v 720281J)C5 = NOT INCLUDED - no result calculated)C6 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D113 v 7579696)E1 = NOT INCLUDED - no result calculated)E2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.D398 v 7579696)E3 = NOT INCLUDED - no result calculated)E4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. D77 v 7579696)E5 = NOT INCLUDED - no result calculated)E6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard1)B1 = NOT INCLUDED - no result calculated)B2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard2)B3 = NOT INCLUDED - no result calculated)B4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard3)B5 = NOT INCLUDED - no result calculated)B6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard4)D1 = NOT INCLUDED - no result calculated)D2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard5)D3 = NOT INCLUDED - no result calculated)D4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard6)D5 = NOT INCLUDED - no result calculated)D6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard7)F1 = NOT INCLUDED - no result calculated)F2 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard8)F3 = NOT INCLUDED - no result calculated)F4 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Standard9)F5 = NOT INCLUDED - no result calculated)F6 = NOT INCLUDED - no result calculated&All wells in this group were excluded. Pos Control1 G1 = 4.95605 G2 = 5.00231Average = 9.95836 / 2 = 4.97918 Neg Control1)H1 = NOT INCLUDED - no result calculated)H2 = NOT INCLUDED - no result calculated&All wells in this group were excluded.#Transform (5) Validation ExpressionDetailsModule-C:\Program Files\Dazdaq\Manta\QTransforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 610,392 bytes Created = 25 June 2004, 12:01:40 CalculationsValidates: Pos Control1>2.0Input MatricesControl OI () Not CalculatedDetailsPlate 1 CalculationsPos Control1>2.0 - passed(18 October 2004 11:22:52) Accessing C:\Documents and Settings\Darren Cook\Local Settings\Temporary Internet Files\OLK2\Slow Kinetics (1).ars3(18 October 2004 12:04:40) Calculations, version 12Raw Data Group CalculationsPlate 10nmAnalysisTransform (1) IntegralDetailsModule-C:\Program Files\Dazdaq\Manta\KRTransform.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 720,984 bytes%Created = 17 September 2004, 12:15:18 CalculationsIntegrated area by trapezium method. Using all unflagged points in between and including X = "1.00756" and X = "100", Axes = (Cycle Number, Reading).Input Matrices 0nm (RLU) Output MatricesIntegralDetailsPlate 1 Calculations)sA1 (D133 v 757973E) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 232.412sA2 (D133 v 757973E) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 215.362sA3 (D398 v 757973E) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 262.507sA4 (D398 v 757973E) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 272.272rA5 (D77 v 757973E) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 287.357rA6 (D77 v 757973E) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 206.972mB1 (Standard1) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 36.467mB2 (Standard1) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 35.542mB3 (Standard2) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 41.187mB4 (Standard2) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 39.637mB5 (Standard3) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 41.282mB6 (Standard3) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 54.277sC1 (D113 v 720281J) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 238.872sC2 (D113 v 720281J) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 213.342sC3 (D398 v 720281J) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 201.677sC4 (D398 v 720281J) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 188.397rC5 (D77 v 720281J) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 265.382rC6 (D77 v 720281J) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 324.902nD1 (Standard4) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 160.972mD2 (Standard4) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 57.517mD3 (Standard5) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 68.262mD4 (Standard5) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 64.497mD5 (Standard6) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 69.257mD6 (Standard6) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 63.767sE1 (D113 v 7579696) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 277.262sE2 (D113 v 7579696) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 330.237sE3 (D398 v 7579696) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 247.667sE4 (D398 v 7579696) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 135.447rE5 (D77 v 7579696) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 160.117rE6 (D77 v 7579696) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 249.147mF1 (Standard7) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 39.032mF2 (Standard7) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 48.562mF3 (Standard8) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 54.712mF4 (Standard8) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 52.592mF5 (Standard9) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 48.362mF6 (Standard9) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 49.532qG1 (Pos Control1) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 189.232qG2 (Pos Control1) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 221.087pH1 (Neg Control1) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 38.182pH2 (Neg Control1) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 44.197Group CalculationsIntegralD133 v 757973E A1 = 232.412 A2 = 215.362Average = 447.774 / 2 = 223.887D398 v 757973E A3 = 262.507 A4 = 272.272Average = 534.779 / 2 = 267.39 D77 v 757973E A5 = 287.357 A6 = 206.972Average = 494.329 / 2 = 247.164D113 v 720281J C1 = 238.872 C2 = 213.342Average = 452.214 / 2 = 226.107D398 v 720281J C3 = 201.677 C4 = 188.397Average = 390.074 / 2 = 195.037 D77 v 720281J C5 = 265.382 C6 = 324.902Average = 590.284 / 2 = 295.142D113 v 7579696 E1 = 277.262 E2 = 330.237Average = 607.499 / 2 = 303.75D398 v 7579696 E3 = 247.667 E4 = 135.447Average = 383.114 / 2 = 191.557 D77 v 7579696 E5 = 160.117 E6 = 249.147Average = 409.264 / 2 = 204.632 Standard1 B1 = 36.467 B2 = 35.542Average = 72.009 / 2 = 36.0045 Standard2 B3 = 41.187 B4 = 39.637Average = 80.824 / 2 = 40.412 Standard3 B5 = 41.282 B6 = 54.277Average = 95.559 / 2 = 47.7795 Standard4 D1 = 160.972 D2 = 57.517Average = 218.489 / 2 = 109.245 Standard5 D3 = 68.262 D4 = 64.497Average = 132.759 / 2 = 66.3795 Standard6 D5 = 69.257 D6 = 63.767Average = 133.024 / 2 = 66.512 Standard7 F1 = 39.032 F2 = 48.562Average = 87.594 / 2 = 43.797 Standard8 F3 = 54.712 F4 = 52.592Average = 107.304 / 2 = 53.652 Standard9 F5 = 48.362 F6 = 49.532Average = 97.894 / 2 = 48.947 Pos Control1 G1 = 189.232 G2 = 221.087Average = 410.319 / 2 = 205.159 Neg Control1 H1 = 38.182 H2 = 44.197Average = 82.379 / 2 = 41.1895Transform (2) Matrix ExpressionDetailsModule,C:\Program Files      !"#$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcdefghijklmnopqrstuvwxyz{|}~\Dazdaq\Manta\Transforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 893,014 bytes%Created = 17 September 2004, 12:15:08 Calculations "w/w(0,1)"Input Matrices Integral () Output Matrices Opsonic IndexDetailsPlate 1 Calculations)*A1 (D133 v 757973E) = w/w(0,1) = 6.37321*A2 (D133 v 757973E) = w/w(0,1) = 6.05937*A3 (D398 v 757973E) = w/w(0,1) = 6.37354*A4 (D398 v 757973E) = w/w(0,1) = 6.86914)A5 (D77 v 757973E) = w/w(0,1) = 6.96083)A6 (D77 v 757973E) = w/w(0,1) = 3.81325&B1 (Standard1) = w/w(0,1) = 0.152663&B2 (Standard1) = w/w(0,1) = 0.166596&B3 (Standard2) = w/w(0,1) = 0.204223&B4 (Standard2) = w/w(0,1) = 0.210391&B5 (Standard3) = w/w(0,1) = 0.155557&B6 (Standard3) = w/w(0,1) = 0.167057*C1 (D113 v 720281J) = w/w(0,1) = 1.48394)C2 (D113 v 720281J) = w/w(0,1) = 3.7092*C3 (D398 v 720281J) = w/w(0,1) = 2.95445*C4 (D398 v 720281J) = w/w(0,1) = 2.92102)C5 (D77 v 720281J) = w/w(0,1) = 3.83184)C6 (D77 v 720281J) = w/w(0,1) = 5.09514&D1 (Standard4) = w/w(0,1) = 0.580577&D2 (Standard4) = w/w(0,1) = 0.174169%D3 (Standard5) = w/w(0,1) = 0.27562&D4 (Standard5) = w/w(0,1) = 0.476179%D5 (Standard6) = w/w(0,1) = 0.43254&D6 (Standard6) = w/w(0,1) = 0.255941*E1 (D113 v 7579696) = w/w(0,1) = 7.10345*E2 (D113 v 7579696) = w/w(0,1) = 6.80032*E3 (D398 v 7579696) = w/w(0,1) = 4.52674*E4 (D398 v 7579696) = w/w(0,1) = 2.57543(E5 (D77 v 7579696) = w/w(0,1) = 3.3108)E6 (D77 v 7579696) = w/w(0,1) = 5.03002&F1 (Standard7) = w/w(0,1) = 0.206265&F2 (Standard7) = w/w(0,1) = 0.219651vF3 (Standard8) Could not evaluate: "w/w(0,1)" - The reference to well G3 is not allowed as that well has been flaggedvF4 (Standard8) Could not evaluate: "w/w(0,1)" - The reference to well G4 is not allowed as that well has been flaggedvF5 (Standard9) Could not evaluate: "w/w(0,1)" - The reference to well G5 is not allowed as that well has been flaggedvF6 (Standard9) Could not evaluate: "w/w(0,1)" - The reference to well G6 is not allowed as that well has been flagged(G1 (Pos Control1) = w/w(0,1) = 4.95605(G2 (Pos Control1) = w/w(0,1) = 5.00231WH1 (Neg Control1) Could not evaluate: "w/w(0,1)" - Well offset out of microplate rangeWH2 (Neg Control1) Could not evaluate: "w/w(0,1)" - Well offset out of microplate rangeGroup Calculations Opsonic IndexD133 v 757973E A1 = 6.37321 A2 = 6.05937Average = 12.4326 / 2 = 6.21629D398 v 757973E A3 = 6.37354 A4 = 6.86914Average = 13.2427 / 2 = 6.62134 D77 v 757973E A5 = 6.96083 A6 = 3.81325Average = 10.7741 / 2 = 5.38704D113 v 720281J C1 = 1.48394 C2 = 3.7092Average = 5.19313 / 2 = 2.59657D398 v 720281J C3 = 2.95445 C4 = 2.92102Average = 5.87547 / 2 = 2.93774 D77 v 720281J C5 = 3.83184 C6 = 5.09514Average = 8.92699 / 2 = 4.46349D113 v 7579696 E1 = 7.10345 E2 = 6.80032Average = 13.9038 / 2 = 6.95189D398 v 7579696 E3 = 4.52674 E4 = 2.57543Average = 7.10217 / 2 = 3.55108 D77 v 7579696 E5 = 3.3108 E6 = 5.03002Average = 8.34082 / 2 = 4.17041 Standard1 B1 = 0.152663 B2 = 0.166596Average = 0.31926 / 2 = 0.15963 Standard2 B3 = 0.204223 B4 = 0.210391!Average = 0.414613 / 2 = 0.207307 Standard3 B5 = 0.155557 B6 = 0.167057!Average = 0.322613 / 2 = 0.161307 Standard4 D1 = 0.580577 D2 = 0.174169!Average = 0.754746 / 2 = 0.377373 Standard5 D3 = 0.27562 D4 = 0.476179!Average = 0.751799 / 2 = 0.375899 Standard6 D5 = 0.43254 D6 = 0.255941!Average = 0.688481 / 2 = 0.344241 Standard7 F1 = 0.206265 F2 = 0.219651!Average = 0.425916 / 2 = 0.212958 Standard8&F3 = NOT INCLUDED - calculation error&F4 = NOT INCLUDED - calculation error&All wells in this group were excluded. Standard9&F5 = NOT INCLUDED - calculation error&F6 = NOT INCLUDED - calculation error&All wells in this group were excluded. Pos Control1 G1 = 4.95605 G2 = 5.00231Average = 9.95836 / 2 = 4.97918 Neg Control1&H1 = NOT INCLUDED - calculation error&H2 = NOT INCLUDED - calculation error&All wells in this group were excluded.Transform (3) Auto Flag By WellDetailsModule,C:\Program Files\Dazdaq\Manta\Transforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 893,014 bytes%Created = 17 September 2004, 12:15:08 Calculationsx>1.2Input MatricesOpsonic Index () Not CalculatedB1 (Standard1) B2 (Standard1) B3 (Standard2) B4 (Standard2) B5 (Standard3) B6 (Standard3) D1 (Standard4) D2 (Standard4) D3 (Standard5) D4 (Standard5) D5 (Standard6) D6 (Standard6) F1 (Standard7) F2 (Standard7) F3 (Standard8) F4 (Standard8) F5 (Standard9) F6 (Standard9) G1 (Pos Control1) G2 (Pos Control1) H1 (Neg Control1) H2 (Neg Control1) DetailsPlate 1 CalculationsExpression = x>1.2BA1 (D133 v 757973E) x = 6.37321, Result = TRUE, Well AUTO-FLAGGEDBA2 (D133 v 757973E) x = 6.05937, Result = TRUE, Well AUTO-FLAGGEDBA3 (D398 v 757973E) x = 6.37354, Result = TRUE, Well AUTO-FLAGGEDBA4 (D398 v 757973E) x = 6.86914, Result = TRUE, Well AUTO-FLAGGEDAA5 (D77 v 757973E) x = 6.96083, Result = TRUE, Well AUTO-FLAGGEDAA6 (D77 v 757973E) x = 3.81325, Result = TRUE, Well AUTO-FLAGGEDBC1 (D113 v 720281J) x = 1.48394, Result = TRUE, Well AUTO-FLAGGEDAC2 (D113 v 720281J) x = 3.7092, Result = TRUE, Well AUTO-FLAGGEDBC3 (D398 v 720281J) x = 2.95445, Result = TRUE, Well AUTO-FLAGGEDBC4 (D398 v 720281J) x = 2.92102, Result = TRUE, Well AUTO-FLAGGEDAC5 (D77 v 720281J) x = 3.83184, Result = TRUE, Well AUTO-FLAGGEDAC6 (D77 v 720281J) x = 5.09514, Result = TRUE, Well AUTO-FLAGGEDBE1 (D113 v 7579696) x = 7.10345, Result = TRUE, Well AUTO-FLAGGEDBE2 (D113 v 7579696) x = 6.80032, Result = TRUE, Well AUTO-FLAGGEDBE3 (D398 v 7579696) x = 4.52674, Result = TRUE, Well AUTO-FLAGGEDBE4 (D398 v 7579696) x = 2.57543, Result = TRUE, Well AUTO-FLAGGED@E5 (D77 v 7579696) x = 3.3108, Result = TRUE, Well AUTO-FLAGGEDAE6 (D77 v 7579696) x = 5.03002, Result = TRUE, Well AUTO-FLAGGED#Transform (4) Validation ExpressionDetailsModule-C:\Program Files\Dazdaq\Manta\QTransforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 610,392 bytes%Created = 17 September 2004, 12:15:48 CalculationsValidates: Pos Control1>2.0Input MatricesOpsonic Index () Not CalculatedDetailsPlate 1 CalculationsPos Control1>2.0 - passed*(18 October 2004 12:08:22) Versions Purged2(18 October 2004 12:09:16) Calculations, version 2Raw Data Group CalculationsPlate 10nmAnalysisTransform (1) IntegralDetailsModule-C:\Program Files\Dazdaq\Manta\KRTransform.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 720,984 bytes%Created = 17 September 2004, 12:15:18 CalculationsIntegrated area by trapezium method. Using all unflagged points in between and including X = "1.00756" and X = "100", Axes = (Cycle Number, Reading).Input Matrices 0nm (RLU) Output MatricesIntegralDetailsPlate 1 Calculations)sA1 (D133 v 757973E) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 232.412sA2 (D133 v 757973E) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 215.362sA3 (D398 v 757973E) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 262.507sA4 (D398 v 757973E) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 272.272rA5 (D77 v 757973E) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 287.357rA6 (D77 v 757973E) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 206.972mB1 (Standard1) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 36.467mB2 (Standard1) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 35.542mB3 (Standard2) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 41.187mB4 (Standard2) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 39.637mB5 (Standard3) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 41.282mB6 (Standard3) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 54.277sC1 (D113 v 720281J) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 238.872sC2 (D113 v 720281J) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 213.342sC3 (D398 v 720281J) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 201.677sC4 (D398 v 720281J) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 188.397rC5 (D77 v 720281J) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 265.382rC6 (D77 v 720281J) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 324.902nD1 (Standard4) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 160.972mD2 (Standard4) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 57.517mD3 (Standard5) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 68.262mD4 (Standard5) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 64.497mD5 (Standard6) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 69.257mD6 (Standard6) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 63.767sE1 (D113 v 7579696) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 277.262sE2 (D113 v 7579696) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 330.237sE3 (D398 v 7579696) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 247.667sE4 (D398 v 7579696) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 135.447rE5 (D77 v 7579696) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 160.117rE6 (D77 v 7579696) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 249.147mF1 (Standard7) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 39.032mF2 (Standard7) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 48.562mF3 (Standard8) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 54.712mF4 (Standard8) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 52.592mF5 (Standard9) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 48.362mF6 (Standard9) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 49.532qG1 (Pos Control1) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 189.232qG2 (Pos Control1) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 221.087pH1 (Neg Control1) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 38.182pH2 (Neg Control1) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 44.197Group CalculationsIntegralD133 v 757973E A1 = 232.412 A2 = 215.362Average = 447.774 / 2 = 223.887D398 v 757973E A3 = 262.507 A4 = 272.272Average = 534.779 / 2 = 267.39 D77 v 757973E A5 = 287.357 A6 = 206.972Average = 494.329 / 2 = 247.164D113 v 720281J C1 = 238.872 C2 = 213.342Average = 452.214 / 2 = 226.107D398 v 720281J C3 = 201.677 C4 = 188.397Average = 390.074 / 2 = 195.037 D77 v 720281J C5 = 265.382 C6 = 324.902Average = 590.284 / 2 = 295.142D113 v 7579696 E1 = 277.262 E2 = 330.237Average = 607.499 / 2 = 303.75D398 v 7579696 E3 = 247.667 E4 = 135.447Average = 383.114 / 2 = 191.557 D77 v 7579696 E5 = 160.117 E6 = 249.147Average = 409.264 / 2 = 204.632 Standard1 B1 = 36.467 B2 = 35.542Average = 72.009 / 2 = 36.0045 Standard2 B3 = 41.187 B4 = 39.637Average = 80.824 / 2 = 40.412 Standard3 B5 = 41.282 B6 = 54.277Average = 95.559 / 2 = 47.7795 Standard4 D1 = 160.972 D2 = 57.517Average = 218.489 / 2 = 109.245 Standard5 D3 = 68.262 D4 = 64.497Average = 132.759 / 2 = 66.3795 Standard6 D5 = 69.257 D6 = 63.767Average = 133.024 / 2 = 66.512 Standard7 F1 = 39.032 F2 = 48.562Average = 87.594 / 2 = 43.797 Standard8 F3 = 54.712 F4 = 52.592Average = 107.304 / 2 = 53.652 Standard9 F5 = 48.362 F6 = 49.532Average = 97.894 / 2 = 48.947 Pos Control1 G1 = 189.232 G2 = 221.087Average = 410.319 / 2 = 205.159 Neg Control1 H1 = 38.182 H2 = 44.197Average = 82.379 / 2 = 41.1895Transform (2) Matrix ExpressionDetailsModule,C:\Program Files\Dazdaq\Manta\Transforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 893,014 bytes%Created = 17 September 2004, 12:15:08 Calculations "w/w(0,1)"Input Matrices Integral () Output Matrices Opsonic IndexDetailsPlate 1 Calculations)*A1 (D133 v 757973E) = w/w(0,1) = 6.37321*A2 (D133 v 757973E) = w/w(0,1) = 6.05937*A3 (D398 v 757973E) = w/w(0,1) = 6.37354*A4 (D398 v 757973E) = w/w(0,1) = 6.86914)A5 (D77 v 757973E) = w/w(0,1) = 6.96083)A6 (D77 v 757973E) = w/w(0,1) = 3.81325&B1 (Standard1) = w/w(0,1) = 0.152663&B2 (Standard1) = w/w(0,1) = 0.166596&B3 (Standard2) = w/w(0,1) = 0.204223&B4 (Standard2) = w/w(0,1) = 0.210391&B5 (Standard3) = w/w(0,1) = 0.155557&B6 (Standard3) = w/w(0,1) = 0.167057*C1 (D113 v 720281J) = w/w(0,1) = 1.48394)C2 (D113 v 720281J) = w/w(0,1) = 3.7092*C3 (D398 v 720281J) = w/w(0,1) = 2.95445*C4 (D398 v 720281J) = w/w(0,1) = 2.92102)C5 (D77 v 720281J) = w/w(0,1) = 3.83184)C6 (D77 v 720281J) = w/w(0,1) = 5.09514&D1 (Standard4) = w/w(0,1) = 0.580577&D2 (Standard4) = w/w(0,1) = 0.174169%D3 (Standard5) = w/w(0,1) = 0.27562&D4 (Standard5) = w/w(0,1) = 0.476179%D5 (Standard6) = w/w(0,1) = 0.43254&D6 (Standard6) = w/w(0,1) = 0.255941*E1 (D113 v 7579696) = w/w(0,1) = 7.10345*E2 (D113 v 7579696) = w/w(0,1) = 6.80032*E3 (D398 v 7579696) = w/w(0,1) = 4.52674*E4 (D398 v 7579696) = w/w(0,1) = 2.57543(E5 (D77 v 7579696) = w/w(0,1) = 3.3108)E6 (D77 v 7579696) = w/w(0,1) = 5.03002&F1 (Standard7) = w/w(0,1) = 0.206265&F2 (Standard7) = w/w(0,1) = 0.219651vF3 (Standard8) Could not evaluate: "w/w(0,1)" - The reference to well G3 is not allowed as that well has been flaggedvF4 (Standard8) Could not evaluate: "w/w(0,1)" - The reference to well G4 is not allowed as that well has been flaggedvF5 (Standard9) Could not evaluate: "w/w(0,1)" - The reference to well G5 is not allowed as that well has been flaggedvF6 (Standard9) Could not evaluate: "w/w(0,1)" - The reference to well G6 is not allowed as that well has been flagged(G1 (Pos Control1) = w/w(0,1) = 4.95605(G2 (Pos Control1) = w/w(0,1) = 5.00231WH1 (Neg Control1) Could not evaluate: "w/w(0,1)" - Well offset out of microplate rangeWH2 (Neg Control1) Could not evaluate: "w/w(0,1)" - Well offset out of microplate rangeGroup Calculations Opsonic IndexD133 v 757973E A1 = 6.37321 A2 = 6.05937Average = 12.4326 / 2 = 6.21629D398 v 757973E A3 = 6.37354 A4 = 6.86914Average = 13.2427 / 2 = 6.62134 D77 v 757973E A5 = 6.96083 A6 = 3.81325Average = 10.7741 / 2 = 5.38704D113 v 720281J C1 = 1.48394 C2 = 3.7092Average = 5.19313 / 2 = 2.59657D398 v 720281J C3 = 2.95445 C4 = 2.92102Average = 5.87547 / 2 = 2.93774 D77 v 720281J C5 = 3.83184 C6 = 5.09514Average = 8.92699 / 2 = 4.46349D113 v 7579696 E1 = 7.10345 E2 = 6.80032Average = 13.9038 / 2 = 6.95189D398 v 7579696 E3 = 4.52674 E4 = 2.57543Average = 7.10217 / 2 = 3.55108 D77 v 7579696 E5 = 3.3108 E6 = 5.03002Average = 8.34082 / 2 = 4.17041 Standard1 B1 = 0.152663 B2 = 0.166596Average = 0.31926 / 2 = 0.15963 Standard2 B3 = 0.204223 B4 = 0.210391!Average = 0.414613 / 2 = 0.207307 Standard3 B5 = 0.155557 B6 = 0.167057!Average = 0.322613 / 2 = 0.161307 Standard4 D1 = 0.580577 D2 = 0.174169!Average = 0.754746 / 2 = 0.377373 Standard5 D3 = 0.27562 D4 = 0.476179!Average = 0.751799 / 2 = 0.375899 Standard6 D5 = 0.43254 D6 = 0.255941!Average = 0.688481 / 2 = 0.344241 Standard7 F1 = 0.206265 F2 = 0.219651!Average = 0.425916 / 2 = 0.212958 Standard8&F3 = NOT INCLUDED - calculation error&F4 = NOT INCLUDED - calculation error&All wells in this group were excluded. Standard9&F5 = NOT INCLUDED - calculation error&F6 = NOT INCLUDED - calculation error&All wells in this group were excluded. Pos Control1 G1 = 4.95605 G2 = 5.00231Average = 9.95836 / 2 = 4.97918 Neg Control1&H1 = NOT INCLUDED - calculation error&H2 = NOT INCLUDED - calculation error&All wells in this group were excluded.#Transform (3) Validation ExpressionDetailsModule-C:\Program Files\Dazdaq\Manta\QTransforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 610,392 bytes%Created = 17 September 2004, 12:15:48 CalculationsValidates: Pos Control1>2.0Input MatricesOpsonic Index () Not CalculatedDetailsPlate 1 CalculationsPos Control1>2.0 - passedTransform (4) Single Cut-OffDetailsModule,C:\Program Files\Dazdaq\Manta\Transforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 893,014 bytes%Created = 17 September 2004, 12:15:08 CalculationsCut-off point = "1.2"Input MatricesOpsonic Index () DetailsPlate 1 CalculationsCut-off point = "1.2" = 1.2Unknown1 = 6.21629 = PositiveUnknown2 = 6.62134 = PositiveUnknown3 = 5.38704 = PositiveUnknown4 = 2.59657 = PositiveUnknown5 = 2.93774 = PositiveUnknown6 = 4.46349 = PositiveUnknown7 = 6.95189 = PositiveUnknown8 = 3.55108 = PositiveUnknown9 = 4.17041 = PositiveStandard1 = 0.15963 = NegativeStandard2 = 0.207307 = NegativeStandard3 = 0.161307 = NegativeStandard4 = 0.377373 = NegativeStandard5 = 0.375899 = NegativeStandard6 = 0.344241 = NegativeStandard7 = 0.212958 = NegativeStandard8 = 0 = NegativeStandard9 = 0 = Negative!Pos Control1 = 4.97918 = PositiveNeg Control1 = 0 = Negative2(18 October 2004 12:13:09) Calculations, version 3Raw Data Group CalculationsPlate 10nmAnalysisTransform (1) IntegralDetailsModule-C:\Program Files\Dazdaq\Manta\KRTransform.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 720,984 bytes%Created = 17 September 2004, 12:15:18 CalculationsIntegrated area by trapezium method. Using all unflagged points in between and including X = "1.00756" and X = "100", Axes = (Cycle Number, Reading).Input Matrices 0nm (RLU) Output MatricesIntegralDetailsPlate 1 Calculations)sA1 (D133 v 757973E) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 232.412sA2 (D133 v 757973E) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 215.362sA3 (D398 v 757973E) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 262.507sA4 (D398 v 757973E) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 272.272rA5 (D77 v 757973E) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 287.357rA6 (D77 v 757973E) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 206.972mB1 (Standard1) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 36.467mB2 (Standard1) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 35.542mB3 (Standard2) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 41.187mB4 (Standard2) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 39.637mB5 (Standard3) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 41.282mB6 (Standard3) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 54.277sC1 (D113 v 720281J) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 238.872sC2 (D113 v 720281J) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 213.342sC3 (D398 v 720281J) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 201.677sC4 (D398 v 720281J) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 188.397rC5 (D77 v 720281J) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 265.382rC6 (D77 v 720281J) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 324.902nD1 (Standard4) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 160.972mD2 (Standard4) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 57.517mD3 (Standard5) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 68.262mD4 (Standard5) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 64.497mD5 (Standard6) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 69.257mD6 (Standard6) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 63.767sE1 (D113 v 7579696) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 277.262sE2 (D113 v 7579696) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 330.237sE3 (D398 v 7579696) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 247.667sE4 (D398 v 7579696) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 135.447rE5 (D77 v 7579696) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 160.117rE6 (D77 v 7579696) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 249.147mF1 (Standard7) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 39.032mF2 (Standard7) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 48.562mF3 (Standard8) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 54.712mF4 (Standard8) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 52.592mF5 (Standard9) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 48.362mF6 (Standard9) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 49.532qG1 (Pos Control1) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 189.232qG2 (Pos Control1) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 221.087pH1 (Neg Control1) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 38.182pH2 (Neg Control1) Points within range 1.00756 and 100. Integral calculated by Trapezium method; Result = 44.197Group CalculationsIntegralD133 v 757973E A1 = 232.412 A2 = 215.362Average = 447.774 / 2 = 223.887D398 v 757973E A3 = 262.507 A4 = 272.272Average = 534.779 / 2 = 267.39 D77 v 757973E A5 = 287.357 A6 = 206.972Average = 494.329 / 2 = 247.165D113 v 720281J C1 = 238.872 C2 = 213.342Average = 452.214 / 2 = 226.107D398 v 720281J C3 = 201.677 C4 = 188.397Average = 390.074 / 2 = 195.037 D77 v 720281J C5 = 265.382 C6 = 324.902Average = 590.284 / 2 = 295.142D113 v 7579696 E1 = 277.262 E2 = 330.237Average = 607.499 / 2 = 303.749D398 v 7579696 E3 = 247.667 E4 = 135.447Average = 383.114 / 2 = 191.557 D77 v 7579696 E5 = 160.117 E6 = 249.147Average = 409.264 / 2 = 204.632 Standard1 B1 = 36.467 B2 = 35.542Average = 72.009 / 2 = 36.0045 Standard2 B3 = 41.187 B4 = 39.637Average = 80.824 / 2 = 40.412 Standard3 B5 = 41.282 B6 = 54.277Average = 95.559 / 2 = 47.7795 Standard4 D1 = 160.972 D2 = 57.517Average = 218.489 / 2 = 109.245 Standard5 D3 = 68.262 D4 = 64.497Average = 132.759 / 2 = 66.3795 Standard6 D5 = 69.257 D6 = 63.767Average = 133.024 / 2 = 66.512 Standard7 F1 = 39.032 F2 = 48.562Average = 87.594 / 2 = 43.797 Standard8 F3 = 54.712 F4 = 52.592Average = 107.304 / 2 = 53.652 Standard9 F5 = 48.362 F6 = 49.532Average = 97.894 / 2 = 48.947 Pos Control1 G1 = 189.232 G2 = 221.087Average = 410.319 / 2 = 205.159 Neg Control1 H1 = 38.182 H2 = 44.197Average = 82.379 / 2 = 41.1895Transform (2) Matrix ExpressionDetailsModule,C:\Program Files\Dazdaq\Manta\Transforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 893,014 bytes%Created = 17 September 2004, 12:15:08 Calculations "w/w(0,1)"Input Matrices Integral () Output Matrices Opsonic IndexDetailsPlate 1 Calculations)*A1 (D133 v 757973E) = w/w(0,1) = 6.37321*A2 (D133 v 757973E) = w/w(0,1) = 6.05937*A3 (D398 v 757973E) = w/w(0,1) = 6.37354*A4 (D398 v 757973E) = w/w(0,1) = 6.86914)A5 (D77 v 757973E) = w/w(0,1) = 6.96083)A6 (D77 v 757973E) = w/w(0,1) = 3.81325&B1 (Standard1) = w/w(0,1) = 0.152663&B2 (Standard1) = w/w(0,1) = 0.166596&B3 (Standard2) = w/w(0,1) = 0.204223&B4 (Standard2) = w/w(0,1) = 0.210391&B5 (Standard3) = w/w(0,1) = 0.155557&B6 (Standard3) = w/w(0,1) = 0.167057*C1 (D113 v 720281J) = w/w(0,1) = 1.48394)C2 (D113 v 720281J) = w/w(0,1) = 3.7092*C3 (D398 v 720281J) = w/w(0,1) = 2.95445*C4 (D398 v 720281J) = w/w(0,1) = 2.92102)C5 (D77 v 720281J) = w/w(0,1) = 3.83184)C6 (D77 v 720281J) = w/w(0,1) = 5.09514&D1 (Standard4) = w/w(0,1) = 0.580577&D2 (Standard4) = w/w(0,1) = 0.174169%D3 (Standard5) = w/w(0,1) = 0.27562&D4 (Standard5) = w/w(0,1) = 0.476179%D5 (Standard6) = w/w(0,1) = 0.43254&D6 (Standard6) = w/w(0,1) = 0.255941*E1 (D113 v 7579696) = w/w(0,1) = 7.10345*E2 (D113 v 7579696) = w/w(0,1) = 6.80032*E3 (D398 v 7579696) = w/w(0,1) = 4.52674*E4 (D398 v 7579696) = w/w(0,1) = 2.57543(E5 (D77 v 7579696) = w/w(0,1) = 3.3108)E6 (D77 v 7579696) = w/w(0,1) = 5.03002&F1 (Standard7) = w/w(0,1) = 0.206265&F2 (Standard7) = w/w(0,1) = 0.219651vF3 (Standard8) Could not evaluate: "w/w(0,1)" - The reference to well G3 is not allowed as that well has been flaggedvF4 (Standard8) Could not evaluate: "w/w(0,1)" - The reference to well G4 is not allowed as that well has been flaggedvF5 (Standard9) Could not evaluate: "w/w(0,1)" - The reference to well G5 is not allowed as that well has been flaggedvF6 (Standard9) Could not evaluate: "w/w(0,1)" - The reference to well G6 is not allowed as that well has been flagged(G1 (Pos Control1) = w/w(0,1) = 4.95605(G2 (Pos Control1) = w/w(0,1) = 5.00231WH1 (Neg Control1) Could not evaluate: "w/w(0,1)" - Well offset out of microplate rangeWH2 (Neg Control1) Could not evaluate: "w/w(0,1)" - Well offset out of microplate rangeGroup Calculations Opsonic IndexD133 v 757973E A1 = 6.37321 A2 = 6.05937Average = 12.4326 / 2 = 6.21629D398 v 757973E A3 = 6.37354 A4 = 6.86914Average = 13.2427 / 2 = 6.62134 D77 v 757973E A5 = 6.96083 A6 = 3.81325Average = 10.7741 / 2 = 5.38704D113 v 720281J C1 = 1.48394 C2 = 3.7092Average = 5.19313 / 2 = 2.59657D398 v 720281J C3 = 2.95445 C4 = 2.92102Average = 5.87547 / 2 = 2.93774 D77 v 720281J C5 = 3.83184 C6 = 5.09514Average = 8.92699 / 2 = 4.46349D113 v 7579696 E1 = 7.10345 E2 = 6.80032Average = 13.9038 / 2 = 6.95189D398 v 7579696 E3 = 4.52674 E4 = 2.57543Average = 7.10217 / 2 = 3.55108 D77 v 7579696 E5 = 3.3108 E6 = 5.03002Average = 8.34082 / 2 = 4.17041 Standard1 B1 = 0.152663 B2 = 0.166596Average = 0.31926 / 2 = 0.15963 Standard2 B3 = 0.204223 B4 = 0.210391!Average = 0.414613 / 2 = 0.207307 Standard3 B5 = 0.155557 B6 = 0.167057!Average = 0.322613 / 2 = 0.161307 Standard4 D1 = 0.580577 D2 = 0.174169!Average = 0.754746 / 2 = 0.377373 Standard5 D3 = 0.27562 D4 = 0.476179!Average = 0.751799 / 2 = 0.375899 Standard6 D5 = 0.43254 D6 = 0.255941!Average = 0.688481 / 2 = 0.344241 Standard7 F1 = 0.206265 F2 = 0.219651!Average = 0.425916 / 2 = 0.212958 Standard8&F3 = NOT INCLUDED - calculation error&F4 = NOT INCLUDED - calculation error&All wells in this group were excluded. Standard9&F5 = NOT INCLUDED - calculation error&F6 = NOT INCLUDED - calculation error&All wells in this group were excluded. Pos Control1 G1 = 4.95605 G2 = 5.00231Average = 9.95836 / 2 = 4.97918 Neg Control1&H1 = NOT INCLUDED - calculation error&H2 = NOT INCLUDED - calculation error&All wells in this group were excluded.#Transform (3) Validation ExpressionDetailsModule-C:\Program Files\Dazdaq\Manta\QTransforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 610,392 bytes%Created = 17 September 2004, 12:15:48 CalculationsValidates: Pos Control1>2.0Input MatricesOpsonic Index () Not CalculatedDetailsPlate 1 CalculationsPos Control1>2.0 - passedTransform (4) Single Cut-OffDetailsModule,C:\Program Files\Dazdaq\Manta\Transforms.dll4Info = Dazdaq Ltd. Manta Module, Dazdaq Ltd., v0.2.2Size = 893,014 bytes%Created = 17 September 2004, 12:15:08 CalculationsCut-off point = "1.2"Input MatricesOpsonic Index () Not CalculatedDetailsPlate 1 CalculationsCut-off point = "1.2" = 1.2Unknown1 = 6.21629 = PositiveUnknown2 = 6.62134 = PositiveUnknown3 = 5.38704 = PositiveUnknown4 = 2.59657 = PositiveUnknown5 = 2.93774 = PositiveUnknown6 = 4.46349 = PositiveUnknown7 = 6.95189 = PositiveUnknown8 = 3.55108 = PositiveUnknown9 = 4.17041 = PositiveStandard1 = 0.15963 = NegativeStandard2 = 0.207307 = NegativeStandard3 = 0.161307 = NegativeStandard4 = 0.377373 = NegativeStandard5 = 0.375899 = NegativeStandard6 = 0.344241 = NegativeStandard7 = 0.212958 = NegativeStandard8 = 0 = NegativeStandard9 = 0 = Negative!Pos Control1 = 4.97918 = PositiveNeg Control1 = 0 = Negative*(18 October 2004 12:22:56) Versions Purged